Rapid, Selective, and Ultra-Sensitive Electronic Field Effect Detection of Escherichia coli

Liena Zaidan; Inna Novodchuk; Alexandru Nica; Alexander H.Xu; Christopher Lloyd; Reza Karimi; Joe Sanderson; Michal Bajcsy; Mustafa Yavuz

doi:10.20944/preprints202406.1630.v1

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preprints.org > engineering > electrical and electronic engineering > doi: 10.20944/preprints202406.1630.v1

Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Rapid, Selective, and Ultra-Sensitive Electronic Field Effect Detection of Escherichia coli

^* ,

Version 1 : Received: 21 June 2024 / Approved: 24 June 2024 / Online: 24 June 2024 (08:43:50 CEST)

How to cite: Zaidan, L.; Novodchuk, I.; Nica, A.; H.Xu, A.; Lloyd, C.; Karimi, R.; Sanderson, J.; Bajcsy, M.; Yavuz, M. Rapid, Selective, and Ultra-Sensitive Electronic Field Effect Detection of Escherichia coli. Preprints 2024, 2024061630. https://doi.org/10.20944/preprints202406.1630.v1 Zaidan, L.; Novodchuk, I.; Nica, A.; H.Xu, A.; Lloyd, C.; Karimi, R.; Sanderson, J.; Bajcsy, M.; Yavuz, M. Rapid, Selective, and Ultra-Sensitive Electronic Field Effect Detection of Escherichia coli. Preprints 2024, 2024061630. https://doi.org/10.20944/preprints202406.1630.v1

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MDPI and ACS Style

Zaidan, L.; Novodchuk, I.; Nica, A.; H.Xu, A.; Lloyd, C.; Karimi, R.; Sanderson, J.; Bajcsy, M.; Yavuz, M. Rapid, Selective, and Ultra-Sensitive Electronic Field Effect Detection of Escherichia coli. Preprints 2024, 2024061630. https://doi.org/10.20944/preprints202406.1630.v1

APA Style

Zaidan, L., Novodchuk, I., Nica, A., H.Xu, A., Lloyd, C., Karimi, R., Sanderson, J., Bajcsy, M., & Yavuz, M. (2024). Rapid, Selective, and Ultra-Sensitive Electronic Field Effect Detection of <em>Escherichia coli</em>. Preprints. https://doi.org/10.20944/preprints202406.1630.v1

Chicago/Turabian Style

Zaidan, L., Michal Bajcsy and Mustafa Yavuz. 2024 "Rapid, Selective, and Ultra-Sensitive Electronic Field Effect Detection of <em>Escherichia coli</em>" Preprints. https://doi.org/10.20944/preprints202406.1630.v1

Abstract

We report the detection of live Escherichia coli (E. coli) in liquid samples using a biosensor based on a field effect transistor (FET) biosensor with B/N co-coped graphene oxide (GO) gel (BN-GO) as the transducer material. The FET was functionalized with antibodies to detect E. coli K12 O-antigens in phosphate-buffered saline (PBS). The biosensor detected the presence of planktonic E. coli bacterial cells within a mere 2 minutes. The biosensor exhibited a limit of detection (LOD) of 10 cells per sample, which can be extrapolated to a limit of detection at the level of a single cell per sample and a detection range of at least 10– 108 CFU/mL. The selectivity of the biosensor for E. coli was demonstrated using Bacillus thuringiensis (B. thuringiensis) as a sample contaminant. We also present a comparison of our functionalized BN-GO FET biosensor with established detection methods of E. coli k12 bacteria, as well as with state-of-the-art detection mechanisms.

Keywords

Graphene; FET; Biosensor; E.coli

Subject

Engineering, Electrical and Electronic Engineering

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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