Tang, D.; Wu, S.; Kong, M.; Liu, Z.; Li, Z.; Han, Y.; Gong, Y.; Hu, J. A Fluorescent Lateral Flow Immunoassay for the Detection of Skeletal Muscle Troponin I in Serum for Muscle Injury Monitoring at the Point of Care. Biosensors2024, 14, 381.
Tang, D.; Wu, S.; Kong, M.; Liu, Z.; Li, Z.; Han, Y.; Gong, Y.; Hu, J. A Fluorescent Lateral Flow Immunoassay for the Detection of Skeletal Muscle Troponin I in Serum for Muscle Injury Monitoring at the Point of Care. Biosensors 2024, 14, 381.
Tang, D.; Wu, S.; Kong, M.; Liu, Z.; Li, Z.; Han, Y.; Gong, Y.; Hu, J. A Fluorescent Lateral Flow Immunoassay for the Detection of Skeletal Muscle Troponin I in Serum for Muscle Injury Monitoring at the Point of Care. Biosensors2024, 14, 381.
Tang, D.; Wu, S.; Kong, M.; Liu, Z.; Li, Z.; Han, Y.; Gong, Y.; Hu, J. A Fluorescent Lateral Flow Immunoassay for the Detection of Skeletal Muscle Troponin I in Serum for Muscle Injury Monitoring at the Point of Care. Biosensors 2024, 14, 381.
Abstract
Exercise-induced muscle injury is one of the most common types of sports injuries. Skeletal muscle troponin I (skTnI) serves as an ideal biomarker in assessing such injuries, facilitating timely detection and evaluation. In this study, we develop a fluorescent sandwich lateral flow immunoassay (LFIA) combined with a desktop analyzer for rapid detection of skTnI. Through optimizing the reaction system, the assay achieves a satisfying detection performance, reaching a limit of detection (LOD) of 0.5 ng/mL with a turnaround time of 15 minutes. The proposed de-tection platform offers portability, ease of use, and high sensitivity, which facilitates the moni-toring of exercise-induced muscle injuries at the point of care. This feature is particularly advan-tageous for end-users, enabling timely detection of sports-related injuries and ultimately en-hancing prognosis and sports life.
Copyright:
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