Wu, Z.; Liu, S.; An, B.; Zhang, H.; Wu, J.; Li, C.; Long, Y. Mutagenesis and Flowering Promotion through Sodium Azide In Vitro Culture of Cymbidium faberi Rolfe. Horticulturae2024, 10, 889.
Wu, Z.; Liu, S.; An, B.; Zhang, H.; Wu, J.; Li, C.; Long, Y. Mutagenesis and Flowering Promotion through Sodium Azide In Vitro Culture of Cymbidium faberi Rolfe. Horticulturae 2024, 10, 889.
Wu, Z.; Liu, S.; An, B.; Zhang, H.; Wu, J.; Li, C.; Long, Y. Mutagenesis and Flowering Promotion through Sodium Azide In Vitro Culture of Cymbidium faberi Rolfe. Horticulturae2024, 10, 889.
Wu, Z.; Liu, S.; An, B.; Zhang, H.; Wu, J.; Li, C.; Long, Y. Mutagenesis and Flowering Promotion through Sodium Azide In Vitro Culture of Cymbidium faberi Rolfe. Horticulturae 2024, 10, 889.
Abstract
Cymbidium faberi Rolfe is one of the traditional Chinese orchids with important ornamental value, and the cultivation of Cymbidium faberi Rolfe mutant strains with different appearances is essential to increase its economic value, but at present, the acquisition of Cymbidium faberi Rolfe mutant strains basically relies on natural mutation.The objectives of this research were to mutagenize Cymbidium faberi Rolfe protocorm-like bodies (PLBs) and shoots in vitro using sodium azide (NaN3), and to screen and evaluate mutants in mutagenized Cymbidium faberi Rolfe seedlings using morphological characteristics.Cymbidium faberi Rolfe PLBs and shoots were used as mutagenic materials, and co-cultivated in induced shoots growth medium with the addition of 0.0 (control), 0.5, 1.0, 1.5, 2.0, 2.5, 3.0 mg/L and 0.0 (control), 4.0, 5.0, 6.0, 7.0, 8.0, 9.0, and 10.0 mg/L NaN3 to induce the mutations, and the mortality rates of the PLBs and shoots were increased with the increase of the NaN3 concentration.At 14 d of co-cultivation, PLBs and shoots were most efficiently mutagenized with 1.5 mg/L and 4.0 mg/L NaN3, respectively.After the explants were cultured for 3 months, changes in leaf and flower morphology were observed in some mutants: shorter and thicker leaves, shorter node length, reduced height, and mid-translucent leaves compared with controls; and some Cymbidium faberi Rolfe bloomed prematurely, with single flowers with large, thick petal sepals and small inflorescences, and colours include light green throughout, and a few with purple stamens.This suggests that NaN3 can effectively mutagenise Cymbidium faberi Rolfe PLBs and shoots to satisfy people's demand for Cymbidium faberi Rolfe's ornamental properties while increasing its economic value.
Keywords
Cymbidium faberi Rolfe; Tissue Culture; NaN3; Mutagenesis; Early Flowering
Subject
Biology and Life Sciences, Horticulture
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
