Wu, Y.-Q.; Ding, K.-X.; Cao, Z.-Y.; Zhao, K.; Gao, H.-Y.; Lv, Z.-C.; Sun, H.-Y.; Li, J.-J.; Li, S.-Y.; Zhao, X.-W.; Xue, Y.; Xiang, S.-S.; Yang, X.-M.; Zheng, X.-F.; Li, C.-Y. Single-Cell Transcriptomics Reveals Early Effects of Ionizing Radiation on Bone Marrow Mononuclear Cells in Mice. Preprints2024, 2024070321. https://doi.org/10.20944/preprints202407.0321.v1
APA Style
Wu, Y. Q., Ding, K. X., Cao, Z. Y., Zhao, K., Gao, H. Y., Lv, Z. C., Sun, H. Y., Li, J. J., Li, S. Y., Zhao, X. W., Xue, Y., Xiang, S. S., Yang, X. M., Zheng, X. F., & Li, C. Y. (2024). Single-Cell Transcriptomics Reveals Early Effects of Ionizing Radiation on Bone Marrow Mononuclear Cells in Mice. Preprints. https://doi.org/10.20944/preprints202407.0321.v1
Chicago/Turabian Style
Wu, Y., Xiao-Fei Zheng and Chang-Yan Li. 2024 "Single-Cell Transcriptomics Reveals Early Effects of Ionizing Radiation on Bone Marrow Mononuclear Cells in Mice" Preprints. https://doi.org/10.20944/preprints202407.0321.v1
Abstract
Ionizing radiation exposure can cause damage to diverse tissues and organs, with the hematopoietic system being the most sensitive. However, limited information is available regarding the radiosensitivity of various hematopoietic cell populations in the bone marrow due to the high heterogeneity of the hematopoietic system. In this study, we observed that granulocyte-macrophage progenitors, hematopoietic stem/progenitor cells and B cells within the bone marrow showed the highest sensitivity, exhibiting a rapid decrease in cell numbers following irradiation. Nonetheless, neutrophils, natural killer (NK) cells, T cells, and dendritic cells demonstrated a certain degree of radioresistance, with neutrophils exhibiting the most pronounced resistance. By employing single-cell transcriptome sequencing, we investigated the early responsive genes in various cell types following irradiation, revealing that distinct gene expression profiles emerged between radiosensitive and radioresistant cells. In B cells, radiation exposure led to a specific upregulation of genes associated with mitochondrial respiratory chain complexes, suggesting a connection between these complexes and cell radiosensitivity. In neutrophils, radiation exposure resulted in fewer gene alterations, indicating their potential for distinct mechanisms in radiation resistance. Collectively, this study provides insights into the molecular mechanism for the heterogeneity of radiosensitivity among the various bone marrow hematopoietic cell populations.
Keywords
irradiation; single-cell transcriptome sequencing; bone marrow mononuclear cells; radiosensitivity
Subject
Biology and Life Sciences, Cell and Developmental Biology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
