Version 1
: Received: 3 July 2024 / Approved: 3 July 2024 / Online: 4 July 2024 (09:12:41 CEST)
How to cite:
Misztal, T.; Roszkowicz-Ostrowska, K.; Kowalczyk, P.; Młotkowska, P.; Marciniak, E. Kynurenic Acid Modulates the Expression of Genes and Activity of Cellular Antioxidant Enzymes in the Hypothalamus and Hippocampus in Sheep. Preprints2024, 2024070366. https://doi.org/10.20944/preprints202407.0366.v1
Misztal, T.; Roszkowicz-Ostrowska, K.; Kowalczyk, P.; Młotkowska, P.; Marciniak, E. Kynurenic Acid Modulates the Expression of Genes and Activity of Cellular Antioxidant Enzymes in the Hypothalamus and Hippocampus in Sheep. Preprints 2024, 2024070366. https://doi.org/10.20944/preprints202407.0366.v1
Misztal, T.; Roszkowicz-Ostrowska, K.; Kowalczyk, P.; Młotkowska, P.; Marciniak, E. Kynurenic Acid Modulates the Expression of Genes and Activity of Cellular Antioxidant Enzymes in the Hypothalamus and Hippocampus in Sheep. Preprints2024, 2024070366. https://doi.org/10.20944/preprints202407.0366.v1
APA Style
Misztal, T., Roszkowicz-Ostrowska, K., Kowalczyk, P., Młotkowska, P., & Marciniak, E. (2024). Kynurenic Acid Modulates the Expression of Genes and Activity of Cellular Antioxidant Enzymes in the Hypothalamus and Hippocampus in Sheep. Preprints. https://doi.org/10.20944/preprints202407.0366.v1
Chicago/Turabian Style
Misztal, T., Patrycja Młotkowska and Elżbieta Marciniak. 2024 "Kynurenic Acid Modulates the Expression of Genes and Activity of Cellular Antioxidant Enzymes in the Hypothalamus and Hippocampus in Sheep" Preprints. https://doi.org/10.20944/preprints202407.0366.v1
Abstract
Kynurenic acid (KYNA), a tryptophan metabolite, is believed to exert neuromodulatory and neuroprotective effects in the brain. The study aimed to examine the KYNA’s capacity to modify gene expression and the activity of cellular antioxidant enzymes in specific structures of the sheep brain. Anestrous sheep were infused intracerebroventricularly with two KYNA doses: lower (4×5 μg/60 μL/30 min, KYNA20) and higher (4×25 μg/60 μL/30 min, KYNA100) at 30min intervals. The abundance of superoxide dismutase (SOD2), catalase (CAT) and glutathione peroxidase (GPx1) mRNA, as well as enzyme activities (µM/µg of protein), were determined in the hypothalamic medial-basal (MBH) and preoptic (POA) areas, and in the hippocampal CA1 field. Both doses of KYNA caused a decrease (P<0.01) in the expression of SOD2 and CAT mRNA in all structures examined compared to the control group (except for CAT in the POA at the KYNA100 dose). Furthermore, lower levels of SOD2 mRNA (P<0.05) and CAT mRNA (P<0.01) were found in the MBH and POA and in the POA and CA, respectively, in sheep administered with the KYNA20 dose. Different stimulatory effects on GPx1 mRNA expression were observed for both doses (P<0.05-P<0.01). KYNA exerted stimulatory, but dose-dependent effects on SOD2, CAT and GPx1 activities (P<0.05-P<0.01) in all brain tissues examined. The results indicate that KYNA may influence the level of oxidative stress in individual brain structures in sheep by modulating the expression of genes and activity of at least SOD2, CAT and GPx1. The present findings also expand the general knowledge about the potential neuroprotective properties of KYNA in the central nervous system.
Biology and Life Sciences, Neuroscience and Neurology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
