Version 1
: Received: 3 July 2024 / Approved: 4 July 2024 / Online: 4 July 2024 (07:03:09 CEST)
How to cite:
Li, H.; Li, K.; Li, H.; Yang, C.; Perera, G.; Wang, G.; Lyu, S.; Hua, L.; Rehman, S. U.; Zhang, Y.; Ayliffe, M.; Yu, H.; Chen, S. Mapping and Candidate Gene Analysis of an All-Stage Stem Rust Resistance Gene in Durum Wheat Landrace PI 94701. Preprints2024, 2024070406. https://doi.org/10.20944/preprints202407.0406.v1
Li, H.; Li, K.; Li, H.; Yang, C.; Perera, G.; Wang, G.; Lyu, S.; Hua, L.; Rehman, S. U.; Zhang, Y.; Ayliffe, M.; Yu, H.; Chen, S. Mapping and Candidate Gene Analysis of an All-Stage Stem Rust Resistance Gene in Durum Wheat Landrace PI 94701. Preprints 2024, 2024070406. https://doi.org/10.20944/preprints202407.0406.v1
Li, H.; Li, K.; Li, H.; Yang, C.; Perera, G.; Wang, G.; Lyu, S.; Hua, L.; Rehman, S. U.; Zhang, Y.; Ayliffe, M.; Yu, H.; Chen, S. Mapping and Candidate Gene Analysis of an All-Stage Stem Rust Resistance Gene in Durum Wheat Landrace PI 94701. Preprints2024, 2024070406. https://doi.org/10.20944/preprints202407.0406.v1
APA Style
Li, H., Li, K., Li, H., Yang, C., Perera, G., Wang, G., Lyu, S., Hua, L., Rehman, S. U., Zhang, Y., Ayliffe, M., Yu, H., & Chen, S. (2024). Mapping and Candidate Gene Analysis of an All-Stage Stem Rust Resistance Gene in Durum Wheat Landrace PI 94701. Preprints. https://doi.org/10.20944/preprints202407.0406.v1
Chicago/Turabian Style
Li, H., Haitao Yu and Shisheng Chen. 2024 "Mapping and Candidate Gene Analysis of an All-Stage Stem Rust Resistance Gene in Durum Wheat Landrace PI 94701" Preprints. https://doi.org/10.20944/preprints202407.0406.v1
Abstract
Puccinia graminis f. sp. tritici (Pgt), the causal agent of wheat stem rust, poses a significant threat to global wheat production. Genetic resistance offers a cost-effective and sustainable solution. The durum wheat landrace PI 94701 was previously hypothesized to carry two stem rust resistance (Sr) genes, but their chromosomal locations were unknown. In this study, we mapped and characterized an all-stage Sr gene in PI 94701, temporarily designated as SrPI94701. In seedling tests, SrPI94701 was effective against all six Pgt races tested. Using a large segregating population (2,302 gametes), we mapped SrPI94701 within a 0.17-cM region flanked by markers pku69124 and pku69228, corresponding to 1.04- and 2.15-Mb genomic regions in the Svevo and Chinese Spring reference genomes. Within the candidate region, eight genes exhibited differential expression between the Pgt-inoculated resistant and susceptible plants. Among them, two NLR genes, TraesCS5B03G1334700 and TraesCS5B03G1335100, showed high polymorphism between parental lines, with upregulation observed in the resistant F4 line compared to its susceptible sister line. However, the flanking and completely linked markers developed in this study could not accurately predict the presence of SrPI94701 in uncharacterized wheat genotypes. SrPI94701 holds promise as a valuable component in breeding programs aimed at enhancing resistance against Pgt.
Keywords
durum wheat; stem rust; resistance gene; SrPI94701; BSR-Seq; CC-NBS-LRR
Subject
Biology and Life Sciences, Agricultural Science and Agronomy
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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