Preprint Article Version 1 This version is not peer-reviewed

Near-Infrared Spectroscopic Determination of Pentacyclic Triterpenoid Concentrations in Additives for Animal Food

Version 1 : Received: 4 July 2024 / Approved: 4 July 2024 / Online: 4 July 2024 (15:18:35 CEST)

How to cite: Sugráñez-Pérez, C.; Sugráñez-Serrano, R.; López-González, M.; Martínez-Vaquero, S.; Moral-Martos, D.; Cortés-Jiménez, S.; Peragón-Sánchez, J. Near-Infrared Spectroscopic Determination of Pentacyclic Triterpenoid Concentrations in Additives for Animal Food. Preprints 2024, 2024070461. https://doi.org/10.20944/preprints202407.0461.v1 Sugráñez-Pérez, C.; Sugráñez-Serrano, R.; López-González, M.; Martínez-Vaquero, S.; Moral-Martos, D.; Cortés-Jiménez, S.; Peragón-Sánchez, J. Near-Infrared Spectroscopic Determination of Pentacyclic Triterpenoid Concentrations in Additives for Animal Food. Preprints 2024, 2024070461. https://doi.org/10.20944/preprints202407.0461.v1

Abstract

The nutritional composition of food for animal production can be completed using olive tree and plant by-products due to their high contents of bioactive compounds, such as pentacyclic triterpenes. Here, we present a novel application of near-infrared spectroscopy (NIRS) for the prediction of the total or individual [maslinic acid (MA), oleanolic acid (OA), and uvaol (UO)] pentacyclic triterpene concentrations in a feed additive obtained from a plant mixture. The oxygen radical absorbance capacity of these types of samples demonstrated the existence of a high antioxidant capacity. The conventional determination methods of pentacyclic triterpenes concentration are costly, labor-intensive, and not practical for analyzing several lines within a limited timeframe at the factory level. The optimal regression model developed in our work demonstrated high correlation values for the calibration and validation sets, along with a high residual prediction deviation value. The NIRS method can be applied directly to dried powder and avoids extraction and high-performance liquid chromatography (HPLC) analysis. Our results also demonstrate that NIRS can accurately quantify pentacyclic triterpenes even at low concentrations in food additives. It can be used at the factory level to directly determine the concentrations in the additive powder obtained at the same time that the powder is produced.

Keywords

Triterpenoids; maslinic acid; oleanolic acid; uvaol; near-infrared spectroscopy; NIRS; ORAC

Subject

Biology and Life Sciences, Biochemistry and Molecular Biology

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