Version 1
: Received: 8 July 2024 / Approved: 9 July 2024 / Online: 10 July 2024 (11:24:53 CEST)
How to cite:
Seglab, F.; Abou Assali, M.; AlYafei, T.; Hassan, H.; Pinto, D. C.; Baydoun, S.; Al Thani, A. A.; Shaito, A. A. A Terpenoid-Rich Fraction of Inula viscosa: Its Chemical Composition, Antioxidant Capacity, and Anticancerous Effects Against Human Lung Cancer Cells. Preprints2024, 2024070755. https://doi.org/10.20944/preprints202407.0755.v1
Seglab, F.; Abou Assali, M.; AlYafei, T.; Hassan, H.; Pinto, D. C.; Baydoun, S.; Al Thani, A. A.; Shaito, A. A. A Terpenoid-Rich Fraction of Inula viscosa: Its Chemical Composition, Antioxidant Capacity, and Anticancerous Effects Against Human Lung Cancer Cells. Preprints 2024, 2024070755. https://doi.org/10.20944/preprints202407.0755.v1
Seglab, F.; Abou Assali, M.; AlYafei, T.; Hassan, H.; Pinto, D. C.; Baydoun, S.; Al Thani, A. A.; Shaito, A. A. A Terpenoid-Rich Fraction of Inula viscosa: Its Chemical Composition, Antioxidant Capacity, and Anticancerous Effects Against Human Lung Cancer Cells. Preprints2024, 2024070755. https://doi.org/10.20944/preprints202407.0755.v1
APA Style
Seglab, F., Abou Assali, M., AlYafei, T., Hassan, H., Pinto, D. C., Baydoun, S., Al Thani, A. A., & Shaito, A. A. (2024). A Terpenoid-Rich Fraction of <em>Inula viscosa</em>: Its Chemical Composition, Antioxidant Capacity, and Anticancerous Effects Against Human Lung Cancer Cells. Preprints. https://doi.org/10.20944/preprints202407.0755.v1
Chicago/Turabian Style
Seglab, F., Asma A. Al Thani and Abdullah A. Shaito. 2024 "A Terpenoid-Rich Fraction of <em>Inula viscosa</em>: Its Chemical Composition, Antioxidant Capacity, and Anticancerous Effects Against Human Lung Cancer Cells" Preprints. https://doi.org/10.20944/preprints202407.0755.v1
Abstract
Inula viscosa is a widely used plant in traditional Mediterranean and Middle Eastern medicine for various illnesses. I. viscosa has been shown to have anticancerous effects against various cancers, but its effects against lung cancer have been under limited investigation. At the same time, I. viscosa is rich in terpenoids whose anti-lung cancer effects have been poorly investigated. This study aimed to examine the potential anticancerous properties of methanolic and aqueous extracts of stems and leaves of I. viscosa and its terpenoid-rich fraction against human lung cancer A549 cells. Results showed a high radical scavenging capacity and polyphenol and flavonoid contents of I. viscosa methanolic rather than aqueous extracts. In addition, leaves methanolic extracts (IVLM) showed the highest reduction in viability of A549 cells among all the extracts. IVLM also reduced the viability of human ovarian SK-OV-3, breast MCF-7, liver HepG2, and colorectal HCT116 cancer cells. A terpenoid-rich I. viscosa fraction (IVL DCM), prepared by liquid-liquid separation of IVLM in dichloromethane (DCM), displayed a substantial reduction in the viability of A549 cells (IC50= 27.8 ± 1.5 µg/mL at 48 h) and the panel of tested cancerous cell lines but was not cytotoxic to normal human embryonic fibroblasts (HDFn). The assessment of IVL DCM phytochemical constituents using GC-MS analysis revealed 21 metabolites, highlighting an enrichment in terpenoids, such as lupeol and its derivatives, caryophyllene oxide, betulin, and isopulegol, known to exhibit proapoptotic, and antimetastatic functions. IVL DCM also showed robust antioxidant capacity and decent polyphenol and flavonoid contents. Furthermore, Western blotting analysis indicated that IVL DCM reduced proliferation (reduction of proliferation marker Ki67 and induction of proliferation inhibitor proteins P21 and P27), contaminant with P38 MAP kinase activation, and induced the intrinsic apoptotic pathway (P53/BCL2/BAX/ Caspase3/PARP) in A549 cells. IVL DCM also reduced the migration of A549 cells, potentially by reducing FAK activation. Future identification of anticancer metabolites of IVL DCM, especially terpenoids, is recommended. These data place I. viscosa as a new resource of herbal anticancer agents.
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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