Zhu, P.-K.; Lin, M.-X.; Zeng, M.-Y.; Tang, Y.; Li, X.-R.; He, T.-Y.; Zheng, Y.-S.; Chen, L.-Y. Potential Regulation of Iron Metabolism Gene Expression by DOF Transcription Factors in Dendrocalamus latiflorus. Preprints2024, 2024070826. https://doi.org/10.20944/preprints202407.0826.v1
APA Style
Zhu, P. K., Lin, M. X., Zeng, M. Y., Tang, Y., Li, X. R., He, T. Y., Zheng, Y. S., & Chen, L. Y. (2024). Potential Regulation of Iron Metabolism Gene Expression by DOF Transcription Factors in <em>Dendrocalamus latiflorus</em>. Preprints. https://doi.org/10.20944/preprints202407.0826.v1
Chicago/Turabian Style
Zhu, P., Yu-Shan Zheng and Ling-Yan Chen. 2024 "Potential Regulation of Iron Metabolism Gene Expression by DOF Transcription Factors in <em>Dendrocalamus latiflorus</em>" Preprints. https://doi.org/10.20944/preprints202407.0826.v1
Abstract
Abstract: Transcription factors are crucial pre-transcriptional regulatory mechanisms that can modulate the expression of downstream genes by binding to their promoter regions. DOF (DNA binding with One Finger) proteins are a unique class of transcription factors with extensive roles in plant growth and development. Our previous research indicated a decrease in iron content in chlorotic bamboo leaves. However, to our knowledge, genes related to iron metabolism pathways in bamboo species have not yet been studied. Therefore, in the current study, we identified iron metabolism-related (IMR) genes in bamboo and determined the transcription factors that significantly influence them. Among these, DOFs were found to have widespread effects and potentially significant impacts on their expression. We identified specific DOF members in Dendrocalamus latiflorus with binding abilities through homology with Arabidopsis DOF proteins, and established connections between some of these members and IMR genes using RNA-seq data. Additionally, molecular docking confirmed the binding interactions between these DlDOFs and the DOF binding sites in the promoter regions of IMR genes. The co-expression relationship between the two gene sets was further validated using q-PCR experiments. This study paves the way for research into iron metabolism pathways in bamboo and lays the foundation for understanding the role of DOF transcription factors in D. latiflorus.
Keywords
DOF transcription factors; Iron Metabolism Related Genes; gene regulation; gene expression; Dendrocalamus latiflorus
Subject
Biology and Life Sciences, Plant Sciences
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
,
,
,
,
,
,
