PreprintArticleVersion 1This version is not peer-reviewed
Solvent Polarity Effects on Phenolic Fingerprint and Biological Activities, Using Three Different Extractions Formulation for Examining of Mentha aquatica Leaf Extracts: An In Vitro and In‐Silico Investigation
Version 1
: Received: 9 July 2024 / Approved: 10 July 2024 / Online: 10 July 2024 (11:47:35 CEST)
How to cite:
TOURABI, M.; Metouekel, A.; Jeddi, M.; Chebaibi, M.; Benkhaira, N.; Fikri Benbrahim, K.; Lyoussi, B.; Derwich, E. Solvent Polarity Effects on Phenolic Fingerprint and Biological Activities, Using Three Different Extractions Formulation for Examining of Mentha aquatica Leaf Extracts: An In Vitro and In‐Silico Investigation. Preprints2024, 2024070842. https://doi.org/10.20944/preprints202407.0842.v1
TOURABI, M.; Metouekel, A.; Jeddi, M.; Chebaibi, M.; Benkhaira, N.; Fikri Benbrahim, K.; Lyoussi, B.; Derwich, E. Solvent Polarity Effects on Phenolic Fingerprint and Biological Activities, Using Three Different Extractions Formulation for Examining of Mentha aquatica Leaf Extracts: An In Vitro and In‐Silico Investigation. Preprints 2024, 2024070842. https://doi.org/10.20944/preprints202407.0842.v1
TOURABI, M.; Metouekel, A.; Jeddi, M.; Chebaibi, M.; Benkhaira, N.; Fikri Benbrahim, K.; Lyoussi, B.; Derwich, E. Solvent Polarity Effects on Phenolic Fingerprint and Biological Activities, Using Three Different Extractions Formulation for Examining of Mentha aquatica Leaf Extracts: An In Vitro and In‐Silico Investigation. Preprints2024, 2024070842. https://doi.org/10.20944/preprints202407.0842.v1
APA Style
TOURABI, M., Metouekel, A., Jeddi, M., Chebaibi, M., Benkhaira, N., Fikri Benbrahim, K., Lyoussi, B., & Derwich, E. (2024). Solvent Polarity Effects on Phenolic Fingerprint and Biological Activities, Using Three Different Extractions Formulation for Examining of <em>Mentha aquatica</em> Leaf Extracts: An In Vitro and In‐Silico Investigation. Preprints. https://doi.org/10.20944/preprints202407.0842.v1
Chicago/Turabian Style
TOURABI, M., Badiaa Lyoussi and Elhoussine Derwich. 2024 "Solvent Polarity Effects on Phenolic Fingerprint and Biological Activities, Using Three Different Extractions Formulation for Examining of <em>Mentha aquatica</em> Leaf Extracts: An In Vitro and In‐Silico Investigation" Preprints. https://doi.org/10.20944/preprints202407.0842.v1
Abstract
The main purpose of our research is the evaluation of the efficacity of solvent polarity on phenolic composition, as well as the antibacterial, and antioxidant activities of extract of Moroccan Mentha aquatica L., leaves. To attempt these objectives, the HPLC-DAD was used to identify the biomolecules. The total amount of phenolic and flavonoid content was determined using the Folin-Ciocalteu and aluminum trichloride processes as well as the DPPH, RP, and TAC tests were used to evaluate the antioxidant capabilities. In addition, by employing both in vitro and in silico analysis through the microdilution method, the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the extracts were assessed against multidrug-resistant bacteria. Furthermore, the High-Performance Liquid Chromatography (HPLC) analysis indicated that all extracts of M. aquatica were abundant in noteworthy phytochemical compounds, especially gallic acid, vanillic acid, chlorobenzoic acid, quercetin, and kaempferol. Furthermore, the results indicated that the total phenolic content was higher in hydroethanolic extract (62.19 ± 1.19 mg GAE/g DW) in contrast to the acetonic and aqueous extracts (22.22 ± 0.64, and 22.44± 0.57 mg GAE/g DW) respectively. Similarly, the aqueous ethanol extract contained the greatest flavonoid concentration, with values of 29.15± 0.09 mg QE/g DW when compared with the other extracts. The seam extract poses excellent antioxidant power with an IC50 of 0.06 ± 0.00 mg/mL for the DPPH test, and lower RP with EC50 of 80± 0.00 µg/ml when compared with acetonic and aqueous extract (370± 0.00 and 460± 0.01 µg/mL) respectively. Moreover, the hydroethanolic extract has a high level of TAC 75.09± 2.00 mg EAA/g DW, followed by aqueous extract carrying a value of 28.53 ± 1.36 mg EAA/g DW. The antibacterial capacity is ranged between 0.78 ± 0.05 mg/mL and 12.60 ± 0.00 mg/L. The results of the in-silico study demonstrated that quercetin, vanillic acid, and naringenin components contribute to the antioxidant capability. While gallic acid, kaempferol, and quercetin components are attributed to the antibacterial capacity.
Biology and Life Sciences, Biochemistry and Molecular Biology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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