Version 1
: Received: 9 July 2024 / Approved: 10 July 2024 / Online: 10 July 2024 (12:59:18 CEST)
How to cite:
Bonet Estruch, E.; López-Lara, M. J.; Gutiérrez-Cortizo, E. N.; Castaño López, M. A.; Mata, P.; Romero-Jiménez, M. J. Total Cholesterol Determination Accuracy in Dried Blood Spots. Preprints2024, 2024070863. https://doi.org/10.20944/preprints202407.0863.v1
Bonet Estruch, E.; López-Lara, M. J.; Gutiérrez-Cortizo, E. N.; Castaño López, M. A.; Mata, P.; Romero-Jiménez, M. J. Total Cholesterol Determination Accuracy in Dried Blood Spots. Preprints 2024, 2024070863. https://doi.org/10.20944/preprints202407.0863.v1
Bonet Estruch, E.; López-Lara, M. J.; Gutiérrez-Cortizo, E. N.; Castaño López, M. A.; Mata, P.; Romero-Jiménez, M. J. Total Cholesterol Determination Accuracy in Dried Blood Spots. Preprints2024, 2024070863. https://doi.org/10.20944/preprints202407.0863.v1
APA Style
Bonet Estruch, E., López-Lara, M. J., Gutiérrez-Cortizo, E. N., Castaño López, M. A., Mata, P., & Romero-Jiménez, M. J. (2024). Total Cholesterol Determination Accuracy in Dried Blood Spots. Preprints. https://doi.org/10.20944/preprints202407.0863.v1
Chicago/Turabian Style
Bonet Estruch, E., Pedro Mata and Manuel J Romero-Jiménez. 2024 "Total Cholesterol Determination Accuracy in Dried Blood Spots" Preprints. https://doi.org/10.20944/preprints202407.0863.v1
Abstract
BACKGROUND
Detecting total cholesterol in dried blood spots could aid in identifying individuals with a high likelihood of familial hypercholesterolemia and could be used as a screening measure. This study aims to assess the diagnostic accuracy of dried blood spots on Whatman 903 paper cards using a manual enzymatic technique.
METHODS
A total of 394 samples collected as serum and dried blood spots were compared. Cholesterol was determined in serum using the automated reference method, while cholesterol on paper was measured using a manual enzymatic method. Within- and between-day diagnostic variability were analyzed. The correlation between both methods was assessed using Passing-Bablok regression and Bland-Altman plot. Internal validation of our correlation formula was performed on 149 samples, along with external validation of the formula proposed by Corso et al.
RESULTS
The within- and between-day coefficient of variation was found to be lower than 10.14% and 14.09%, respectively. Method comparison yielded an area under the curve of 0.976, with a standard error of 0.009. Passing-Bablok regression indicated a precision of 0.803 and an accuracy of 0.96. Internal validation precision was measured at 0.716. Regarding internal validation, calculated cholesterol with our regression line did not differ from serum cholesterol analysis, while significant differences were observed in calculated cholesterol levels using the external formula.
CONCLUSIONS
Total cholesterol analysis in dot blood spots demonstrates high precision and reproducibility. This method reliably enables the incorporation of this biological marker into neonatal screening for familial hypercholesterolemia detection.
Keywords
total blood cholesterol; dried blood spots; familial hypercholesterolemia; newborn screening.
Subject
Medicine and Pharmacology, Clinical Medicine
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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