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The Potential of CRISPR/Cas9 to Circumvent the Risk Factor Neurotoxin β-ODAP Limiting Wide Acceptance of the Underutilized Grass Pea (Lathyrus sativus L).
Version 1
: Received: 9 July 2024 / Approved: 10 July 2024 / Online: 11 July 2024 (12:15:04 CEST)
How to cite:
Ligaba-Osena, A. The Potential of CRISPR/Cas9 to Circumvent the Risk Factor Neurotoxin β-ODAP Limiting Wide Acceptance of the Underutilized Grass Pea (Lathyrus sativus L).. Preprints2024, 2024070890. https://doi.org/10.20944/preprints202407.0890.v1
Ligaba-Osena, A. The Potential of CRISPR/Cas9 to Circumvent the Risk Factor Neurotoxin β-ODAP Limiting Wide Acceptance of the Underutilized Grass Pea (Lathyrus sativus L).. Preprints 2024, 2024070890. https://doi.org/10.20944/preprints202407.0890.v1
Ligaba-Osena, A. The Potential of CRISPR/Cas9 to Circumvent the Risk Factor Neurotoxin β-ODAP Limiting Wide Acceptance of the Underutilized Grass Pea (Lathyrus sativus L).. Preprints2024, 2024070890. https://doi.org/10.20944/preprints202407.0890.v1
APA Style
Ligaba-Osena, A. (2024). The Potential of CRISPR/Cas9 to Circumvent the Risk Factor Neurotoxin β-ODAP Limiting Wide Acceptance of the Underutilized Grass Pea (<em>Lathyrus sativus L</em>).. Preprints. https://doi.org/10.20944/preprints202407.0890.v1
Chicago/Turabian Style
Ligaba-Osena, A. 2024 "The Potential of CRISPR/Cas9 to Circumvent the Risk Factor Neurotoxin β-ODAP Limiting Wide Acceptance of the Underutilized Grass Pea (<em>Lathyrus sativus L</em>)." Preprints. https://doi.org/10.20944/preprints202407.0890.v1
Abstract
Grass pea (Lathyrus sativus L.) is a protein-rich crop that is resilient to various abiotic stresses, including drought. However, it is not cultivated widely for human consumption due to the neurotoxin β-N-oxalyl-L-α, β-diaminopropionic acid (β-ODAP), and its association with neurolathyrism. Though some varieties with low β-ODAP have been developed through classical breeding, the β-ODAP content is increasing due to genotype x environment interactions. This review covers grass pea nutritional quality, β-ODAP biosynthesis, mechanism of paralysis, traditional ways to reduce β-ODAP, candidate genes for boosting sulfur-containing amino acids, and the potential and targets of gene editing to reduce β-ODAP content. Recently, two key enzymes (β-ODAP synthase and β-cyanoalanine synthase) have been identified in the biosynthetic pathway of β-ODAP. We proposed four strategies through which the genes encoding these enzymes can be targeted and suppressed using CRISPR/Cas9 gene editing. Compared to its homology in Medicago truncatula, the grass pea β-ODAP synthase gene sequence and β-cyanoalanine synthase showed 62.9% and 95% similarity, respectively. The β-ODAP synthase converts the final intermediate L-DAPA into toxic β-ODAP whist β-cyanoalanine synthase converts O-Acetylserine into β-isoxazolin-5-on-2-yl-alanine. Since grass pea is low in methionine and cysteine amino acids, improvement of these amino acids is also needed to boost its protein content.
Biology and Life Sciences, Biology and Biotechnology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
