PreprintArticleVersion 1This version is not peer-reviewed
Integrated Ultrasound Characterization of the Diet-Induced Obesity (Dio) Model in Young Adult C57bl/6j Mice: Assessment of Cardiovascular, Renal and Hepatic Changes
Version 1
: Received: 10 July 2024 / Approved: 11 July 2024 / Online: 12 July 2024 (04:09:05 CEST)
How to cite:
Gargiulo, S.; Barone, V.; Bonente, D.; Tamborrino, T.; Inzalaco, G.; Gherardini, L.; Bertelli, E.; Chiariello, M. Integrated Ultrasound Characterization of the Diet-Induced Obesity (Dio) Model in Young Adult C57bl/6j Mice: Assessment of Cardiovascular, Renal and Hepatic Changes. Preprints2024, 2024070987. https://doi.org/10.20944/preprints202407.0987.v1
Gargiulo, S.; Barone, V.; Bonente, D.; Tamborrino, T.; Inzalaco, G.; Gherardini, L.; Bertelli, E.; Chiariello, M. Integrated Ultrasound Characterization of the Diet-Induced Obesity (Dio) Model in Young Adult C57bl/6j Mice: Assessment of Cardiovascular, Renal and Hepatic Changes. Preprints 2024, 2024070987. https://doi.org/10.20944/preprints202407.0987.v1
Gargiulo, S.; Barone, V.; Bonente, D.; Tamborrino, T.; Inzalaco, G.; Gherardini, L.; Bertelli, E.; Chiariello, M. Integrated Ultrasound Characterization of the Diet-Induced Obesity (Dio) Model in Young Adult C57bl/6j Mice: Assessment of Cardiovascular, Renal and Hepatic Changes. Preprints2024, 2024070987. https://doi.org/10.20944/preprints202407.0987.v1
APA Style
Gargiulo, S., Barone, V., Bonente, D., Tamborrino, T., Inzalaco, G., Gherardini, L., Bertelli, E., & Chiariello, M. (2024). Integrated Ultrasound Characterization of the Diet-Induced Obesity (Dio) Model in Young Adult C57bl/6j Mice: Assessment of Cardiovascular, Renal and Hepatic Changes. Preprints. https://doi.org/10.20944/preprints202407.0987.v1
Chicago/Turabian Style
Gargiulo, S., Eugenio Bertelli and Mario Chiariello. 2024 "Integrated Ultrasound Characterization of the Diet-Induced Obesity (Dio) Model in Young Adult C57bl/6j Mice: Assessment of Cardiovascular, Renal and Hepatic Changes" Preprints. https://doi.org/10.20944/preprints202407.0987.v1
Abstract
Overconsumption of an unbalanced diet and overweight, leading to insulin resistance, dyslipidemia, hepatic steatosis, cardiovascular diseases and obesity, is nowadays a worldwide health issue, with different patterns of metabolic disturbances in the young and adult popula-tions, in both gender. Preclinical models that closely mimic the gradual weight gain that occurs in humans upon consumption of a “western-type” diet (WD) are of great interest. In particular, the diet-induced obesity (DIO) model in the C57BL/6J mouse sub-strain is often used in biomed-ical research, considering that it is prone to metabolic disorders and obesity, and is widely used to generate genetically modified mice. This study aimed to phenotypically characterize the C57BL/6J mouse strain fed a WD-like diet, using high-frequency ultrasound imaging (HFUS) as a complementary tool to longitudinally monitor morphofunctional changes in the liver, heart and kidney associated with DIO. Long term WD feeding increased mice body weight (BW), liver/BW ratio and body condition score (BCS), serum transaminases, glucose and insulin, as well as Homeostasis model assessment (HOMA) indexes, and caused dyslipidemia and insulin resistance. According to the 3Rs, ultrasound imaging has enabled a comprehensive study of metabolic syndrome in mice, in vivo and non-invasive, improving the identification of early stages of diseases.
Keywords
obesity; metabolic syndrome; animal model; C57Bl/6J mouse; high frequency ultrasound imaging
Subject
Medicine and Pharmacology, Other
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
