Version 1
: Received: 12 July 2024 / Approved: 12 July 2024 / Online: 15 July 2024 (07:21:58 CEST)
How to cite:
Rubinstein, A.; Garcia Liñares, G.; Boeris, V.; Perez, O. Innovative Bio-Vehicle For Resveratrol And Tocopherol Based On Quinoa 11s Globulin. Nanocomplexes Design And Characterization. Preprints2024, 2024071087. https://doi.org/10.20944/preprints202407.1087.v1
Rubinstein, A.; Garcia Liñares, G.; Boeris, V.; Perez, O. Innovative Bio-Vehicle For Resveratrol And Tocopherol Based On Quinoa 11s Globulin. Nanocomplexes Design And Characterization. Preprints 2024, 2024071087. https://doi.org/10.20944/preprints202407.1087.v1
Rubinstein, A.; Garcia Liñares, G.; Boeris, V.; Perez, O. Innovative Bio-Vehicle For Resveratrol And Tocopherol Based On Quinoa 11s Globulin. Nanocomplexes Design And Characterization. Preprints2024, 2024071087. https://doi.org/10.20944/preprints202407.1087.v1
APA Style
Rubinstein, A., Garcia Liñares, G., Boeris, V., & Perez, O. (2024). Innovative Bio-Vehicle For Resveratrol And Tocopherol Based On Quinoa 11s Globulin. Nanocomplexes Design And Characterization. Preprints. https://doi.org/10.20944/preprints202407.1087.v1
Chicago/Turabian Style
Rubinstein, A., Valeria Boeris and Oscar Perez. 2024 "Innovative Bio-Vehicle For Resveratrol And Tocopherol Based On Quinoa 11s Globulin. Nanocomplexes Design And Characterization" Preprints. https://doi.org/10.20944/preprints202407.1087.v1
Abstract
The objective of the present contribution was to design and characterize resveratrol (RSV) and tocopherol (TOC) loaded 11S quinoa seed protein nanocomplexes. Firstly, molecular docking was performed to describe the probable binding sites between protein and ligands. Isothermal titration calorimetry allowed to obtain the thermodynamic parameters that described the molecular in-teractions between RSV or TOC with the protein. 11S globulin intrinsic fluorescence spectra showed a quenching effect exerted by RSV and TOC, demonstrating protein-bioactive compounds interactions. Stern-Volmer, Scatchard and Förster resonance energy transfer models application confirmed static quenching and allowed to obtain parameters that described the 11S-RSV or 11S-TOC complexation process. Secondly, protein aggregation induced by bioactive compounds interactions was confirmed by dynamic light scattering and atomic force microscopy with diam-eters
Biology and Life Sciences, Biology and Biotechnology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
