PreprintArticleVersion 1This version is not peer-reviewed
Genomic Characterisation of Methicillin-Resistant and Methicillin-Susceptible Staphylococcus aureus Implicated in Bloodstream Infections, KwaZulu Natal, South Africa
Version 1
: Received: 13 July 2024 / Approved: 15 July 2024 / Online: 15 July 2024 (10:37:36 CEST)
How to cite:
Hetsa, B. A.; Asante, J.; Mbanga, J.; Ismail, A.; Abia, A. L. K.; Amoako, D. G.; Essack, S. Y. Genomic Characterisation of Methicillin-Resistant and Methicillin-Susceptible Staphylococcus aureus Implicated in Bloodstream Infections, KwaZulu Natal, South Africa. Preprints2024, 2024071137. https://doi.org/10.20944/preprints202407.1137.v1
Hetsa, B. A.; Asante, J.; Mbanga, J.; Ismail, A.; Abia, A. L. K.; Amoako, D. G.; Essack, S. Y. Genomic Characterisation of Methicillin-Resistant and Methicillin-Susceptible Staphylococcus aureus Implicated in Bloodstream Infections, KwaZulu Natal, South Africa. Preprints 2024, 2024071137. https://doi.org/10.20944/preprints202407.1137.v1
Hetsa, B. A.; Asante, J.; Mbanga, J.; Ismail, A.; Abia, A. L. K.; Amoako, D. G.; Essack, S. Y. Genomic Characterisation of Methicillin-Resistant and Methicillin-Susceptible Staphylococcus aureus Implicated in Bloodstream Infections, KwaZulu Natal, South Africa. Preprints2024, 2024071137. https://doi.org/10.20944/preprints202407.1137.v1
APA Style
Hetsa, B. A., Asante, J., Mbanga, J., Ismail, A., Abia, A. L. K., Amoako, D. G., & Essack, S. Y. (2024). Genomic Characterisation of Methicillin-Resistant and Methicillin-Susceptible <em>Staphylococcus aureus </em>Implicated in Bloodstream Infections, KwaZulu Natal, South Africa. Preprints. https://doi.org/10.20944/preprints202407.1137.v1
Chicago/Turabian Style
Hetsa, B. A., Daniel G. Amoako and Sabiha Y. Essack. 2024 "Genomic Characterisation of Methicillin-Resistant and Methicillin-Susceptible <em>Staphylococcus aureus </em>Implicated in Bloodstream Infections, KwaZulu Natal, South Africa" Preprints. https://doi.org/10.20944/preprints202407.1137.v1
Abstract
Staphylococcus aureus is an opportunistic human pathogen and a leading cause of bloodstream infections. It can acquire different antibiotic resistance genes, leading to treatment failure. Aim: We elaborate on the genomic characteristics; antibiotic resistance, virulence, pathogenicity, phylogenomics and clonal diversity of S. aureus implicated in bloodstream infections. Six multidrug-resistant (MDR) S. aureus, three methicillin-resistant S. aureus (MRSA) and three methicillin-sensitive S. aureus obtained from blood cultures underwent whole genome sequencing and bioinformatics analysis. All isolates carried different permutations and combinations of resistance genes including, blaZ, mecA, aac(6')-aph (2''), ant(9)-Ia, ant(6)-Ia, mepR, fosB, norA, norC, lmrS, arlS, arlR, mgrA, kdpD and sdrM. We found 6 spa types (t9475, t355, t045, t1265, t1257, and t7888) with varying profiles of virulence genes responsible for immune invasion, enterotoxins, adhesion/biofilm, haemolysins, and leukotoxins. Panton-Valentine leukocidin (Luk-PV) was found in one MSSA isolate. Two SCCmec types IVd(2B) and I(1B) were identified. Isolates belonged to four multilocus sequence types (MLSTs), the most common of which was ST5 (n=3). The STs were clustered into two clonal complexes CC5 and CC8. We found two MRSA clones typed as ST5-CC5-t045-SCCmec_I(1B), and the human-associated MRSA endemic clone ST612-CC8-t1257-SCCmec_IVd(2B). The insertion sequences IS30 and IS6 associated with virulence were found in two isolates. The presence of virulent MDR S. aureus in bloodstream infections poses a clinical concern because of limited treatment options and increased risk of mortality.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
,
,
