Version 1
: Received: 14 July 2024 / Approved: 15 July 2024 / Online: 16 July 2024 (00:16:07 CEST)
How to cite:
Sun, Q.; Ding, S.; Liu, X.; Zhang, W. 中.; Yu, F. 中.; Fu, Y. 中.; Li, Y.; Dong, C. The Co-infection of ISKNV-II and RGNNV Resulting in Mass Mortalities of Juvenile Asian Seabass (Lates calcarifer), Zhuhai, Southern China. Preprints2024, 2024071210. https://doi.org/10.20944/preprints202407.1210.v1
Sun, Q.; Ding, S.; Liu, X.; Zhang, W. 中.; Yu, F. 中.; Fu, Y. 中.; Li, Y.; Dong, C. The Co-infection of ISKNV-II and RGNNV Resulting in Mass Mortalities of Juvenile Asian Seabass (Lates calcarifer), Zhuhai, Southern China. Preprints 2024, 2024071210. https://doi.org/10.20944/preprints202407.1210.v1
Sun, Q.; Ding, S.; Liu, X.; Zhang, W. 中.; Yu, F. 中.; Fu, Y. 中.; Li, Y.; Dong, C. The Co-infection of ISKNV-II and RGNNV Resulting in Mass Mortalities of Juvenile Asian Seabass (Lates calcarifer), Zhuhai, Southern China. Preprints2024, 2024071210. https://doi.org/10.20944/preprints202407.1210.v1
APA Style
Sun, Q., Ding, S., Liu, X., Zhang, W. 中., Yu, F. 中., Fu, Y. 中., Li, Y., & Dong, C. (2024). The Co-infection of ISKNV-II and RGNNV Resulting in Mass Mortalities of Juvenile Asian Seabass (Lates calcarifer), Zhuhai, Southern China. Preprints. https://doi.org/10.20944/preprints202407.1210.v1
Chicago/Turabian Style
Sun, Q., Yong Li and Chuanfu Dong. 2024 "The Co-infection of ISKNV-II and RGNNV Resulting in Mass Mortalities of Juvenile Asian Seabass (Lates calcarifer), Zhuhai, Southern China" Preprints. https://doi.org/10.20944/preprints202407.1210.v1
Abstract
A mass mortality event of Asian seabass Lates calcarifer juveniles occurred in Zhuhai, the main Asian seabass cultured area in mainland China. The fish samples were pooled for pathogen identification and both high viral loads of ISKNV and NNV were detected by real-time microfluidic quantitative PCR. Immunohistochemistry and immunofluorescence showed that strong ISKNV signals were examined in spleen and liver, while strong NNV signals were detected in brain and eye. The multi-tissue homogenates were inoculated into MFF-1 cell and SSN-1 cell, respectively. After several viral passages, both ISKNV and NNV were purely isolated from each other, and designated as ASB-ISKNV-23 and ASB-NNV-23, respectively. The whole genome sequences of ASB-ISKNV-23 and ASB-NNV-23 were determined and annotated. The result showed that ASB-ISKNV-23 and ASB-NNV-23 are composed of 112,236 bp and 4,538 bp, respectively. Phylogeny analysis showed that ASB-ISKNV-23 belongs to ISKNV-II sub-genotype and ASB-NNV-23 belongs to RGNNV genotype. Collectedly, coinfection of ISKNV-II and RGNNV were firstly documented in mass mortality of Asian seabass in mainland China. Importantly, both ISKNV-II and RGNNV were purely isolated using two different permissive cell lines. Our study provides useful information for better understanding the complex pathogenesis regarding the coinfection with ISKNV and NNV in farmed fish.
Keywords
Infectious spleen and kidney necrosis virus genotype II (ISKNV-II); Red grouper type nervous necrosis virus (RGNNV); Asian sea bass Lates calcarifer; Co-infection; Mass mortality.
Subject
Biology and Life Sciences, Virology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
