Herpes Simplex Virus 1 Infection of Human Brain Organoids and Pancreatic Stem Cell-Islets Drives Transcripts Associated with Alzheimer’s Disease and Autoimmune Diseases

How to cite: Sundstrom, J.; Vanderleeden, E.; Barton, N. J.; Redick, S. D.; Dawes, P.; Murray, L. F.; Olson, M. N.; Tran, K.; Chigas, S. M.; Orszulak, A. R.; Church, G. M.; Readhead, B.; Oh, H.; Harlan, D. M.; Knipe, D. M.; Wang, J. P.; Chan, Y.; Lim, E. T. Herpes Simplex Virus 1 Infection of Human Brain Organoids and Pancreatic Stem Cell-Islets Drives Transcripts Associated with Alzheimer’s Disease and Autoimmune Diseases. Preprints 2024, 2024071267. https://doi.org/10.20944/preprints202407.1267.v1 Sundstrom, J.; Vanderleeden, E.; Barton, N. J.; Redick, S. D.; Dawes, P.; Murray, L. F.; Olson, M. N.; Tran, K.; Chigas, S. M.; Orszulak, A. R.; Church, G. M.; Readhead, B.; Oh, H.; Harlan, D. M.; Knipe, D. M.; Wang, J. P.; Chan, Y.; Lim, E. T. Herpes Simplex Virus 1 Infection of Human Brain Organoids and Pancreatic Stem Cell-Islets Drives Transcripts Associated with Alzheimer’s Disease and Autoimmune Diseases. Preprints 2024, 2024071267. https://doi.org/10.20944/preprints202407.1267.v1

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MDPI and ACS Style

Sundstrom, J.; Vanderleeden, E.; Barton, N. J.; Redick, S. D.; Dawes, P.; Murray, L. F.; Olson, M. N.; Tran, K.; Chigas, S. M.; Orszulak, A. R.; Church, G. M.; Readhead, B.; Oh, H.; Harlan, D. M.; Knipe, D. M.; Wang, J. P.; Chan, Y.; Lim, E. T. Herpes Simplex Virus 1 Infection of Human Brain Organoids and Pancreatic Stem Cell-Islets Drives Transcripts Associated with Alzheimer’s Disease and Autoimmune Diseases. Preprints 2024, 2024071267. https://doi.org/10.20944/preprints202407.1267.v1

APA Style

Sundstrom, J., Vanderleeden, E., Barton, N. J., Redick, S. D., Dawes, P., Murray, L. F., Olson, M. N., Tran, K., Chigas, S. M., Orszulak, A. R., Church, G. M., Readhead, B., Oh, H., Harlan, D. M., Knipe, D. M., Wang, J. P., Chan, Y., & Lim, E. T. (2024). Herpes Simplex Virus 1 Infection of Human Brain Organoids and Pancreatic Stem Cell-Islets Drives Transcripts Associated with Alzheimer’s Disease and Autoimmune Diseases. Preprints. https://doi.org/10.20944/preprints202407.1267.v1

Chicago/Turabian Style

Sundstrom, J., Yingleong Chan and Elaine T Lim. 2024 "Herpes Simplex Virus 1 Infection of Human Brain Organoids and Pancreatic Stem Cell-Islets Drives Transcripts Associated with Alzheimer’s Disease and Autoimmune Diseases" Preprints. https://doi.org/10.20944/preprints202407.1267.v1

Abstract

Viral infections leading to inflammation have been implicated in several common diseases such as Alzheimer’s disease (AD) and type 1 diabetes (T1D). Of note, herpes simplex virus 1 (HSV-1) has been reported to be associated with AD. We sought to identify the transcriptomic changes due to HSV-1 infection and anti-viral drug (acyclovir, ACV) treatment of HSV-1 infection in dissoci-ated cells from human cerebral organoids (dcOrgs) versus stem cell-derived pancreatic islets (sc-islets) to gain potential biological insights into the relevance of HSV-1-induced inflammation in AD and T1D. We observed that differentially expressed genes (DEGs) in HSV-1-infected sc-islets were enriched for genes associated with several autoimmune diseases, most significantly T1D but also rheumatoid arthritis, psoriasis, Crohn’s disease, and multiple sclerosis, whereas DEGs in HSV-1-infected dcOrgs were exclusively enriched for genes associated with AD. ACV treatment of sc-islets did not rescue transcript expression of autoimmune disease-associated genes. Finally, we identified gene ontology categories that were enriched for DEGs that were in common across, or unique to, viral treatment of dcOrgs and sc-islets, such as categories involved in the transferase complex, mitochondrial and autophagy function. Collectively, this studies pro-vide insight to the molecular effects of inflammation in AD and T1D.

Keywords

stem cell islets; cerebral organoids; Type 1 diabetes; Alzheimer’s disease; innate immune; neurodegenerative diseases; autoimmune diseases; acyclovir; herpes simplex virus 1

Subject

Biology and Life Sciences, Immunology and Microbiology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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