Li, X.; Tao, H.; Wang, S.; Zhang, D.; Xiong, X.; Cai, Y. IAA Synthesis Pathway of Fitibacillus barbaricus WL35 and Its Regulatory Gene Expression Levels in Potato (Solanum tuberosum L.). Preprints2024, 2024071475. https://doi.org/10.20944/preprints202407.1475.v1
APA Style
Li, X., Tao, H., Wang, S., Zhang, D., Xiong, X., & Cai, Y. (2024). IAA Synthesis Pathway of <em>Fitibacillus </em><em>barbaricus </em>WL35 and Its Regulatory Gene Expression Levels in Potato (<em>Solanum tuberosum</em> L.). Preprints. https://doi.org/10.20944/preprints202407.1475.v1
Chicago/Turabian Style
Li, X., Xingyao Xiong and Yanfei Cai. 2024 "IAA Synthesis Pathway of <em>Fitibacillus </em><em>barbaricus </em>WL35 and Its Regulatory Gene Expression Levels in Potato (<em>Solanum tuberosum</em> L.)" Preprints. https://doi.org/10.20944/preprints202407.1475.v1
Abstract
Indole-3-acetic acid (IAA), as an important regulator of potato growth, seriously affects the growth and yield of potato. Although many studies have reported that IAA-producing Bacillus can promote plant growth, little research has been done on its synthesis pathway and the molecular mechanisms. In this study, IAA-producing strain WL35 was identified as Fitibacillus barbaricus, and its yield was 48.79 mg·L-1. The results of the pot experiments showed that WL35 significantly increased plant height, stem thickness, chlorophyll content and number of leaves of potato plants by 31.68%, 30.03%, 32.93% and 36.59%, respectively. In addition, in the field experiments, WL35-treated plants increased commercial potatoes yield by 16.45%, vitamin C content by 16.35%, protein content by 75%, starch content by 6.60%, nitrogen, phosphorus and potassium accumulation by 9.98%, 12.70% and 26.76%, respectively. Meanwhile, the synthetic pathway of WL35 was found to be dominated by the tryptophan-dependent pathway, the IAM, TAM and IPA pathways worked together, and the pathways that played a role at different times were different. Furthermore, RNA-seq analysis showed that there were a total of 2875 DEGs regulated in the samples treated with WL35 seed dressing compared with the CK, of which 1458 genes were up-regulated and 1417 genes were down-regulated. Potato roots express differential genes enriched in processes such as carbohydrate metabolism processes and cellular polysaccharide metabolism, which regulate potato plant growth and development. The above results provide a theoretical basis for the further exploration of the synthesis pathway of IAA and its growth-promoting mechanism on potato.
Biology and Life Sciences, Immunology and Microbiology
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