PreprintArticleVersion 1This version is not peer-reviewed
The Presence of Selenium Nanoparticles May Enhance the Toxicity of Cadmium to Chlamydomonas reinhardtii – Evidence from Wild-Type and Cell-Deficient Chlamydomonas reinhardtii Cells
Version 1
: Received: 19 July 2024 / Approved: 20 July 2024 / Online: 22 July 2024 (07:20:51 CEST)
How to cite:
Sun, Y.; Gao, M.; Zhu, Y. The Presence of Selenium Nanoparticles May Enhance the Toxicity of Cadmium to Chlamydomonas reinhardtii – Evidence from Wild-Type and Cell-Deficient Chlamydomonas reinhardtii Cells. Preprints2024, 2024071646. https://doi.org/10.20944/preprints202407.1646.v1
Sun, Y.; Gao, M.; Zhu, Y. The Presence of Selenium Nanoparticles May Enhance the Toxicity of Cadmium to Chlamydomonas reinhardtii – Evidence from Wild-Type and Cell-Deficient Chlamydomonas reinhardtii Cells. Preprints 2024, 2024071646. https://doi.org/10.20944/preprints202407.1646.v1
Sun, Y.; Gao, M.; Zhu, Y. The Presence of Selenium Nanoparticles May Enhance the Toxicity of Cadmium to Chlamydomonas reinhardtii – Evidence from Wild-Type and Cell-Deficient Chlamydomonas reinhardtii Cells. Preprints2024, 2024071646. https://doi.org/10.20944/preprints202407.1646.v1
APA Style
Sun, Y., Gao, M., & Zhu, Y. (2024). The Presence of Selenium Nanoparticles May Enhance the Toxicity of Cadmium to <em>Chlamydomonas reinhardtii</em> – Evidence from Wild-Type and Cell-Deficient <em>Chlamydomonas reinhardtii </em>Cells. Preprints. https://doi.org/10.20944/preprints202407.1646.v1
Chicago/Turabian Style
Sun, Y., Min Gao and Yi Zhu. 2024 "The Presence of Selenium Nanoparticles May Enhance the Toxicity of Cadmium to <em>Chlamydomonas reinhardtii</em> – Evidence from Wild-Type and Cell-Deficient <em>Chlamydomonas reinhardtii </em>Cells" Preprints. https://doi.org/10.20944/preprints202407.1646.v1
Abstract
Selenium nanoparticles (SeNPs) can mitigate cadmium (Cd2+) toxicity in crop cultivation and aquaculture. This study investigates the impact of SeNPs on Cd-induced toxicity in Chlamydomonas reinhardtii, a model organism, aiming to mitigate Cd damage in crops. SeNPs were synthesized using sodium carboxymethyl cellulose (CMC) as a dispersant and vitamin C to reduce H2SeO3. Characterization by transmission electron microscopy (TEM) and nanoparticle granulator revealed uniform, stable spherical SeNPs. Cd2+ and SeNPs of varying concentrations were introduced into culture media of wild type (CC125mt+) and cell wall defect type (CC400mt+) Chlamydomonas reinhardtii. Growth curves, photosynthetic pigment content, enzyme activities (T-SOD and CAT), MDA content, and ROS levels were assessed. Fluorescence induction kinetics, QA oxidation kinetics, and Cd2+ content in cells were measured to evaluate SeNPs' effect on Cd toxicity. Results showed SeNPs exacerbated Cd2+ toxicity in CC125mt+ by inhibiting growth and increasing catalase activity, indicating enhanced oxidative damage. SeNPs intensified PSII damage by Cd2+ and increased intracellular Cd2+ content, possibly exacerbating toxicity. SeNPs had no effect on Cd2+ toxicity in CC400mt+. Overall, SeNPs exacerbated Cd2+ toxicity in wild type CC125mt+ but not in cell wall defective CC400mt+. These findings caution against indiscriminate use of nano-selenium to mitigate Cd damage, stressing the importance of careful target selection, dosage, and range considerations.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
