Version 1
: Received: 19 July 2024 / Approved: 22 July 2024 / Online: 22 July 2024 (07:50:13 CEST)
How to cite:
De Capua, A.; Vecchione, R.; Sgambato, C.; Chino, M.; Lombardi, A.; Netti, P. A. Peptide Functionalization of Emulsion-Based Nanocarrier towards Blood Brain Barrier. Preprints2024, 2024071667. https://doi.org/10.20944/preprints202407.1667.v1
De Capua, A.; Vecchione, R.; Sgambato, C.; Chino, M.; Lombardi, A.; Netti, P. A. Peptide Functionalization of Emulsion-Based Nanocarrier towards Blood Brain Barrier. Preprints 2024, 2024071667. https://doi.org/10.20944/preprints202407.1667.v1
De Capua, A.; Vecchione, R.; Sgambato, C.; Chino, M.; Lombardi, A.; Netti, P. A. Peptide Functionalization of Emulsion-Based Nanocarrier towards Blood Brain Barrier. Preprints2024, 2024071667. https://doi.org/10.20944/preprints202407.1667.v1
APA Style
De Capua, A., Vecchione, R., Sgambato, C., Chino, M., Lombardi, A., & Netti, P. A. (2024). Peptide Functionalization of Emulsion-Based Nanocarrier towards Blood Brain Barrier. Preprints. https://doi.org/10.20944/preprints202407.1667.v1
Chicago/Turabian Style
De Capua, A., Angela Lombardi and Paolo Antonio Netti. 2024 "Peptide Functionalization of Emulsion-Based Nanocarrier towards Blood Brain Barrier" Preprints. https://doi.org/10.20944/preprints202407.1667.v1
Abstract
New strategies for enhancing drug delivery to the blood–brain barrier (BBB) represents a major challenge in treating cerebral diseases. Nano-emulsion based nanocarriers represent an ideal candidate to improve drug delivery thanks to their versatility in functionalization and cargo protection. In this work, a paclitaxel loaded nano-emulsion has been firstly functionalized and stabilized with two layers constituted of chitosan and hyaluronic acid, and, secondly, the latter has been conjugated to the CRT peptide. CRT is a bioactive peptide that selectively recognizes bEnd.3 cells, model of the BBB, thanks to its interactions with transferrin (Tf) and its receptor (TfR).
Cytotoxic results showed a higher uptake of CRT functionalized nano-emulsion 41.5 % more than the negative control, demonstrating the ability of this novel tool to be accumulated on the brain endothelium tissue. Thanks to these results, our approach can be fully generalizable to the design of multifunctional nanocarriers for delivery of therapeutic agents to the central nervous systems.
Keywords
active targeting; CRT peptide; drug delivery; cancer; nanoemulsion
Subject
Biology and Life Sciences, Biology and Biotechnology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
