Version 1
: Received: 22 July 2024 / Approved: 23 July 2024 / Online: 24 July 2024 (07:20:12 CEST)
How to cite:
Marabello, D.; Canepa, C.; Cioci, A.; Benzi, P. Beta-Hydroxybutyric Acid as a Template for the X-ray Powder Diffraction Analysis of Gamma-Hydroxybutyric Acid. Preprints2024, 2024071850. https://doi.org/10.20944/preprints202407.1850.v1
Marabello, D.; Canepa, C.; Cioci, A.; Benzi, P. Beta-Hydroxybutyric Acid as a Template for the X-ray Powder Diffraction Analysis of Gamma-Hydroxybutyric Acid. Preprints 2024, 2024071850. https://doi.org/10.20944/preprints202407.1850.v1
Marabello, D.; Canepa, C.; Cioci, A.; Benzi, P. Beta-Hydroxybutyric Acid as a Template for the X-ray Powder Diffraction Analysis of Gamma-Hydroxybutyric Acid. Preprints2024, 2024071850. https://doi.org/10.20944/preprints202407.1850.v1
APA Style
Marabello, D., Canepa, C., Cioci, A., & Benzi, P. (2024). Beta-Hydroxybutyric Acid as a Template for the X-ray Powder Diffraction Analysis of Gamma-Hydroxybutyric Acid. Preprints. https://doi.org/10.20944/preprints202407.1850.v1
Chicago/Turabian Style
Marabello, D., Alma Cioci and Paola Benzi. 2024 "Beta-Hydroxybutyric Acid as a Template for the X-ray Powder Diffraction Analysis of Gamma-Hydroxybutyric Acid" Preprints. https://doi.org/10.20944/preprints202407.1850.v1
Abstract
In this paper we report the possibility of using the X-ray powder diffraction (XRPD) technique to detect the gamma-hydroxybutyric acid (GHB) as its sodium salt in different beverages but because it is not possible to freely buy them, beta-hydroxybutyric acid (BHB) and its sodium salt (NaBHB) were used as a model to fine tune an X-ray diffraction method for the qualitative analysis of the sodium salt of GHB. The method requires a small quantity of beverage, and an easy sample preparation, that consists only in the NaOH addition to the drink and a subsequent drying step. The dry residue obtained can be easily analyzed with XRPD using a single crystal X-ray diffractometer to exploit its high sensitivity that allows very fast patterns collection. Several beverages with different NaBHB:NaOH molar ratios were tested and the results show that NaBHB is detected in all drinks analyzed when NaBHB:NaOH molar ratio is 1:50 using characteristic peak at very low 2θ values that permits to detect its presence also in complex beverage matrices. Moreover, depending on the NaOH amount added, shifting and/or splitting of the characteristic peak of the NaBHB salt was observed, and the origin of this behavior was investigated.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
