Preprint Article Version 1 This version is not peer-reviewed

Impaired Coordination of the Ciliary Movement in Patients with Chronic Rhinosinusitis with Nasal Polyps: The Role of Decreased Planar Cell Polarity Protein Expression

Sakura Hirokane
,
Tomohiro Kawasumi
,
Sachio Takeno
* ,
Yukako Okamoto
,
Seita Miyamoto
,
Rikuto Fujita
,
Chie Ishikawa
,
Takashi Oda
,
Yuichiro Horibe
,
Takashi Ishino
,
Takao Hamamoto
,
Tsutomu Ueda
Version 1 : Received: 23 July 2024 / Approved: 24 July 2024 / Online: 24 July 2024 (08:02:35 CEST)

How to cite: Hirokane, S.; Kawasumi, T.; Takeno, S.; Okamoto, Y.; Miyamoto, S.; Fujita, R.; Ishikawa, C.; Oda, T.; Horibe, Y.; Ishino, T.; Hamamoto, T.; Ueda, T. Impaired Coordination of the Ciliary Movement in Patients with Chronic Rhinosinusitis with Nasal Polyps: The Role of Decreased Planar Cell Polarity Protein Expression. Preprints 2024, 2024071890. https://doi.org/10.20944/preprints202407.1890.v1 Hirokane, S.; Kawasumi, T.; Takeno, S.; Okamoto, Y.; Miyamoto, S.; Fujita, R.; Ishikawa, C.; Oda, T.; Horibe, Y.; Ishino, T.; Hamamoto, T.; Ueda, T. Impaired Coordination of the Ciliary Movement in Patients with Chronic Rhinosinusitis with Nasal Polyps: The Role of Decreased Planar Cell Polarity Protein Expression. Preprints 2024, 2024071890. https://doi.org/10.20944/preprints202407.1890.v1

Abstract

The planar cell polarity (PCP) of epithelial ciliated cells is essential for effective mucociliary clearance (MCC) in the sinonasal mucosa. We hypothesize that MCC coordination is impaired in nasal polyp (NP) mucosae due to suppressed expression of a series of CPLANE (ciliogenesis and planar cell polarity effector) complex proteins in chronic rhinosinusitis (CRS) patients. To inves-tigate this hypothesis, we subjected sinonasal mucosal samples to live video recording to measure mucociliary transport velocity (MCTV) and scanning electron microscopy to evaluate surface morphology. The expression and distribution of a panel of PCP proteins, e.g. WDPCP and FUZ, were investigated in relation to inflammatory cytokine levels and clinical features. The mean MCTV of NP mucosae was significantly lower than that of the inferior turbinate mucosae. The CRS group with NPs (CRSwNP group) (n=28) showed increased expression of IL-13 and CCL26 mRNA compared to CRS patients without NPs (n=25) and controls (n=30). WDPCP and FUZ mRNA levels were significantly decreased in NP mucosae compared to ethmoid sinus mucosae in CRSwNP patients. WDPCP protein distribution was reduced in the cytoplasmic region of ciliated cells in CRSwNP patients. We conclude that suppression of WDPCP in ciliated cells is responsible for the impaired MCC of nasal polyps with type-2 inflammation. This mechanism might explain the decreased clearance and the potential for worsening symptoms of CRSwNP.

Keywords

paranasal sinus; chronic rhinosinusitis; CRS; nasal polyps; ciliated cell; mucociliary clearance; planar cell polarity; CPLANE complex proteins; WDPCP; FUZ

Subject

Medicine and Pharmacology, Otolaryngology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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