Version 1
: Received: 24 July 2024 / Approved: 25 July 2024 / Online: 25 July 2024 (15:00:53 CEST)
How to cite:
Zhao, Z.; Xie, Y.; Tian, M.; Liu, J.; Chen, C.; Zhou, J. 中.; Guo, T.; Xiao, W. Enhancing Coleoptile Length of Rice Seeds under Submergence through NAL11 Knockout. Preprints2024, 2024072079. https://doi.org/10.20944/preprints202407.2079.v1
Zhao, Z.; Xie, Y.; Tian, M.; Liu, J.; Chen, C.; Zhou, J. 中.; Guo, T.; Xiao, W. Enhancing Coleoptile Length of Rice Seeds under Submergence through NAL11 Knockout. Preprints 2024, 2024072079. https://doi.org/10.20944/preprints202407.2079.v1
Zhao, Z.; Xie, Y.; Tian, M.; Liu, J.; Chen, C.; Zhou, J. 中.; Guo, T.; Xiao, W. Enhancing Coleoptile Length of Rice Seeds under Submergence through NAL11 Knockout. Preprints2024, 2024072079. https://doi.org/10.20944/preprints202407.2079.v1
APA Style
Zhao, Z., Xie, Y., Tian, M., Liu, J., Chen, C., Zhou, J. 中., Guo, T., & Xiao, W. (2024). Enhancing Coleoptile Length of Rice Seeds under Submergence through NAL11 Knockout. Preprints. https://doi.org/10.20944/preprints202407.2079.v1
Chicago/Turabian Style
Zhao, Z., Tao Guo and Wuming Xiao. 2024 "Enhancing Coleoptile Length of Rice Seeds under Submergence through NAL11 Knockout" Preprints. https://doi.org/10.20944/preprints202407.2079.v1
Abstract
Submergence stress challenges direct seeding in rice cultivation. In this study, we identified a heat shock protein, NAL11, with a DnaJ domain, which can regulate the length of rice coleoptiles under flooded conditions. Through bioinformatics analyses, we identified cis-regulatory elements in its promoter, making it responsive to abiotic stresses, such as hypoxia or anoxia. Expression of NAL11 was higher in the basal regions of shoots and coleoptiles during flooding. NAL11 knockout triggered the rapid accumulation of abscisic acid (ABA) and reduction of Gibberellin (GA), stimulating rice coleoptile elongation and contributes to flooding stress management. In addition, NAL11 mutants were found to be more sensitive to ABA treatments. Such knockout lines exhibited enhanced cell elongation for coleoptile extension. Quantitative RT-PCR analysis revealed that NAL11 mediated the gluconeogenic pathway, essential for the energy needed in cell expansion. Furthermore, NAL11 mutants reduced the accumulation of reactive oxygen species (ROS) and malondialdehyde under submerged stress, attributed to an improved antioxidant enzyme system compared to the wild-type. In conclusion, our findings underscore the pivotal role of NAL11 knockout in enhancing the tolerance of rice to submergence stress by elucidating its mechanisms. This insight offers a new strategy for improving resilience against flooding in rice cultivation.
Biology and Life Sciences, Agricultural Science and Agronomy
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
