PreprintArticleVersion 1This version is not peer-reviewed
Revealing the Therapeutic Potential of Muscle-Derived Mesenchymal Stem/Stroma Cells: An In Vitro Model for Equine Laminitis based on Activated Neutrophils, Anoxia-Reoxygenation and Myeloperoxidase
Version 1
: Received: 25 July 2024 / Approved: 26 July 2024 / Online: 26 July 2024 (08:05:49 CEST)
How to cite:
Serteyn, D.; Storms, N.; Mouithys-Mickalad, A.; Sandersen, C.; Niesten, A.; Duysens, J.; Graide, H.; Ceusters, J.; Franck, T. Revealing the Therapeutic Potential of Muscle-Derived Mesenchymal Stem/Stroma Cells: An In Vitro Model for Equine Laminitis based on Activated Neutrophils, Anoxia-Reoxygenation and Myeloperoxidase. Preprints2024, 2024072148. https://doi.org/10.20944/preprints202407.2148.v1
Serteyn, D.; Storms, N.; Mouithys-Mickalad, A.; Sandersen, C.; Niesten, A.; Duysens, J.; Graide, H.; Ceusters, J.; Franck, T. Revealing the Therapeutic Potential of Muscle-Derived Mesenchymal Stem/Stroma Cells: An In Vitro Model for Equine Laminitis based on Activated Neutrophils, Anoxia-Reoxygenation and Myeloperoxidase. Preprints 2024, 2024072148. https://doi.org/10.20944/preprints202407.2148.v1
Serteyn, D.; Storms, N.; Mouithys-Mickalad, A.; Sandersen, C.; Niesten, A.; Duysens, J.; Graide, H.; Ceusters, J.; Franck, T. Revealing the Therapeutic Potential of Muscle-Derived Mesenchymal Stem/Stroma Cells: An In Vitro Model for Equine Laminitis based on Activated Neutrophils, Anoxia-Reoxygenation and Myeloperoxidase. Preprints2024, 2024072148. https://doi.org/10.20944/preprints202407.2148.v1
APA Style
Serteyn, D., Storms, N., Mouithys-Mickalad, A., Sandersen, C., Niesten, A., Duysens, J., Graide, H., Ceusters, J., & Franck, T. (2024). Revealing the Therapeutic Potential of Muscle-Derived Mesenchymal Stem/Stroma Cells: An In Vitro Model for Equine Laminitis based on Activated Neutrophils, Anoxia-Reoxygenation and Myeloperoxidase. Preprints. https://doi.org/10.20944/preprints202407.2148.v1
Chicago/Turabian Style
Serteyn, D., Justine Ceusters and Thierry Franck. 2024 "Revealing the Therapeutic Potential of Muscle-Derived Mesenchymal Stem/Stroma Cells: An In Vitro Model for Equine Laminitis based on Activated Neutrophils, Anoxia-Reoxygenation and Myeloperoxidase" Preprints. https://doi.org/10.20944/preprints202407.2148.v1
Abstract
Equine laminitis is a debilitating condition characterized by the breakdown of the dermal–epidermal junction, resulting in severe lameness and pain, often requiring euthanasia. This study aimed to establish an in vitro model of laminitis using a continuous keratinocyte cell line exposed to anoxia-reoxygenation and activated neutrophil supernatant. A significant decrease in keratinocytes metabolism was noted during the reoxygenation period, indicative of cellular stress. Adding muscle-derived mesenchymal stem/stroma cells during reoxygenation demonstrated a protective effect, restoring keratinocytes metabolic activity. Moreover, incubation of keratinocytes with activated neutrophil supernatant or myeloperoxidase alone induced increased keratinocyte myeloperoxidase activity, which was modulated by stem cells. These findings underscore the potential of muscle-derived mesenchymal stem/stroma cells in mitigating inflammation and restoring keratinocyte metabolism, offering insights for future cell therapy research in laminitis treatment.
Biology and Life Sciences, Animal Science, Veterinary Science and Zoology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
