Version 1
: Received: 26 July 2024 / Approved: 27 July 2024 / Online: 30 July 2024 (11:53:00 CEST)
How to cite:
Nicoletti, N. F.; Marinowic, D. R.; Perondi, D.; Budelon Gonçalves, J. I.; Piazza, D.; da Costa, J. C.; Falavigna, A. Noncytotoxic Graphene Nanoplatelets Upregulates Cell Proliferation and Self-Renewal Genes of Mesenchymal Stem Cells. Preprints2024, 2024072227. https://doi.org/10.20944/preprints202407.2227.v1
Nicoletti, N. F.; Marinowic, D. R.; Perondi, D.; Budelon Gonçalves, J. I.; Piazza, D.; da Costa, J. C.; Falavigna, A. Noncytotoxic Graphene Nanoplatelets Upregulates Cell Proliferation and Self-Renewal Genes of Mesenchymal Stem Cells. Preprints 2024, 2024072227. https://doi.org/10.20944/preprints202407.2227.v1
Nicoletti, N. F.; Marinowic, D. R.; Perondi, D.; Budelon Gonçalves, J. I.; Piazza, D.; da Costa, J. C.; Falavigna, A. Noncytotoxic Graphene Nanoplatelets Upregulates Cell Proliferation and Self-Renewal Genes of Mesenchymal Stem Cells. Preprints2024, 2024072227. https://doi.org/10.20944/preprints202407.2227.v1
APA Style
Nicoletti, N. F., Marinowic, D. R., Perondi, D., Budelon Gonçalves, J. I., Piazza, D., da Costa, J. C., & Falavigna, A. (2024). Noncytotoxic Graphene Nanoplatelets Upregulates Cell Proliferation and Self-Renewal Genes of Mesenchymal Stem Cells. Preprints. https://doi.org/10.20944/preprints202407.2227.v1
Chicago/Turabian Style
Nicoletti, N. F., Jaderson Costa da Costa and Asdrubal Falavigna. 2024 "Noncytotoxic Graphene Nanoplatelets Upregulates Cell Proliferation and Self-Renewal Genes of Mesenchymal Stem Cells" Preprints. https://doi.org/10.20944/preprints202407.2227.v1
Abstract
Graphene nanoplatelets (UGZ-1004) are advancing as a promising biomaterial for regenerative medicine. This study provides a detailed assessment of UGZ-1004, emphasizing its physical properties, cytotoxicity, intracellular interactions, and particularly its effects on mesenchymal stem cells (MSCs). UGZ-1004 was characterized to have lateral sizes and layer counts in line with ISO standards and exhibited high carbon purity (0.08%). Cytotoxicity evaluations demonstrated that UGZ-1004 is non-toxic to various cell lines, including fibroblast 3T3, epithelial kidney VERO, microglia BV-2, and MSCs, in accordance with ISO-10993-5/2020/2023 guidelines. The study focused on MSCs, revealing that UGZ-1004 supports their self-renewal and proliferation. MSCs exposed to UGZ-1004 maintained their characteristic surface markers and proliferation rates. Importantly, UGZ-1004 promoted significant upregulation of genes crucial for cell cycle regulation and DNA repair, such as CDK1, CDK2, and MDM2. This gene expression profile suggests that UGZ-1004 enhances MSC self-renewal capabilities, ensuring robust cellular function and longevity. Moreover, UGZ-1004 exposure led to the downregulation of genes associated with tumor development, including CCND1 and TFDP1, mitigating potential tumorigenic risks. These findings underscore the potential of UGZ-1004 to not only bolster MSC proliferation but also enhance their self-renewal processes, which are critical for effective regenerative therapies. The study highlights the need for continued research into the long-term impacts of graphene nanoplatelets and their application in MSC-based regenerative medicine.
Biology and Life Sciences, Cell and Developmental Biology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
