Version 1
: Received: 29 July 2024 / Approved: 30 July 2024 / Online: 30 July 2024 (10:30:02 CEST)
How to cite:
Daugaliyeva, S.; Daugaliyeva, A.; Amirova, K.; Yelubayeva, A.; Kapar, A.; Abiti, A.; Partipilo, T.; Toktarov, N.; Peletto, S. Foraging of Honeybees from Different Ecological Areas Determined through Melissopalynological Analysis and DNA Metabarcoding. Preprints2024, 2024072408. https://doi.org/10.20944/preprints202407.2408.v1
Daugaliyeva, S.; Daugaliyeva, A.; Amirova, K.; Yelubayeva, A.; Kapar, A.; Abiti, A.; Partipilo, T.; Toktarov, N.; Peletto, S. Foraging of Honeybees from Different Ecological Areas Determined through Melissopalynological Analysis and DNA Metabarcoding. Preprints 2024, 2024072408. https://doi.org/10.20944/preprints202407.2408.v1
Daugaliyeva, S.; Daugaliyeva, A.; Amirova, K.; Yelubayeva, A.; Kapar, A.; Abiti, A.; Partipilo, T.; Toktarov, N.; Peletto, S. Foraging of Honeybees from Different Ecological Areas Determined through Melissopalynological Analysis and DNA Metabarcoding. Preprints2024, 2024072408. https://doi.org/10.20944/preprints202407.2408.v1
APA Style
Daugaliyeva, S., Daugaliyeva, A., Amirova, K., Yelubayeva, A., Kapar, A., Abiti, A., Partipilo, T., Toktarov, N., & Peletto, S. (2024). Foraging of Honeybees from Different Ecological Areas Determined through Melissopalynological Analysis and DNA Metabarcoding. Preprints. https://doi.org/10.20944/preprints202407.2408.v1
Chicago/Turabian Style
Daugaliyeva, S., Nurlan Toktarov and Simone Peletto. 2024 "Foraging of Honeybees from Different Ecological Areas Determined through Melissopalynological Analysis and DNA Metabarcoding" Preprints. https://doi.org/10.20944/preprints202407.2408.v1
Abstract
The environment significantly impacts the life of bees and their feeding. This study aimed to investigate bee foraging using melissopalynological analysis and DNA metabarcoding in intensive farming, reserved, and urbanized areas. The highest alpha diversity was observed in the reserved and in the intensive farming areas. The urbanized area has less diversity. In the intensive farming area predominated: Sinapis, Helianthus, and Fagopyrum; in the reserved area: Melilotus, Helianthus, and Brassica. In the urbanized area garden plants: radish (Raphanus sativus) and cucumber (Cucumis hystrix) and agricultural plants: soybean (Glycine max) and melon (Cucumis melo) were often found. The most significant agreement was between the rbcL and the melissopalynological analysis. The ITS2 revealed equal matches with both rbcL and melissopalynology but this marker missed or underestimated some genera. Trifolium pretense and Brassica nigra, were identified simultaneously by three analyses. Species Convolvulus arvensis, Melilotus officinalis, Echium vulgare, Brassica rapa, Helianthus divaricatus, and Onobrychis viciifolia were found in all ecological areas. Imperfect databases put some limits for identification of some taxa using metabarcoding. Further research and expansion of plant databases is needed. Studying the food preferences of bees in different environmental conditions and landscapes is necessary to develop measures to preserve their population.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
