Version 1
: Received: 29 July 2024 / Approved: 30 July 2024 / Online: 30 July 2024 (23:57:42 CEST)
How to cite:
Toprak, V.; Özdemir, İ.; Öztürk, Ş.; Yanar, O.; Kızıldemir, Y. Z.; Tuncer, M. C. Analysis of FOXP3 Gene Expression in OVCAR3 Cell Line After Rosmarinic Acid and Doxorubicin Treatment. Preprints2024, 2024072478. https://doi.org/10.20944/preprints202407.2478.v1
Toprak, V.; Özdemir, İ.; Öztürk, Ş.; Yanar, O.; Kızıldemir, Y. Z.; Tuncer, M. C. Analysis of FOXP3 Gene Expression in OVCAR3 Cell Line After Rosmarinic Acid and Doxorubicin Treatment. Preprints 2024, 2024072478. https://doi.org/10.20944/preprints202407.2478.v1
Toprak, V.; Özdemir, İ.; Öztürk, Ş.; Yanar, O.; Kızıldemir, Y. Z.; Tuncer, M. C. Analysis of FOXP3 Gene Expression in OVCAR3 Cell Line After Rosmarinic Acid and Doxorubicin Treatment. Preprints2024, 2024072478. https://doi.org/10.20944/preprints202407.2478.v1
APA Style
Toprak, V., Özdemir, İ., Öztürk, Ş., Yanar, O., Kızıldemir, Y. Z., & Tuncer, M. C. (2024). Analysis of FOXP3 Gene Expression in OVCAR3 Cell Line After Rosmarinic Acid and Doxorubicin Treatment. Preprints. https://doi.org/10.20944/preprints202407.2478.v1
Chicago/Turabian Style
Toprak, V., Yusuf Ziya Kızıldemir and Mehmet Cudi Tuncer. 2024 "Analysis of FOXP3 Gene Expression in OVCAR3 Cell Line After Rosmarinic Acid and Doxorubicin Treatment" Preprints. https://doi.org/10.20944/preprints202407.2478.v1
Abstract
Aim: Ovarian cancer is the leading cause of gynecological cancer deaths worldwide. Treatment methods are listed as surgery, chemotherapy and radiotherapy depending on the stage of the cancer, but developing resistance to chemotherapy increases the need for alternative agents that act on the same pathways. In this study, the effects of rosmarinic acid (RA) and doxorubicin (DX) on the activation of FOXP3, an important tumor suppressor gene, in OVCAR3 cells were examined.
Material Method: In the study, human ovarian adenocarcinoma cell line was used. MTT test was applied to determine the effect of rosmarinic acid and doxorubicin on the proliferation of OVCAR3 cells. Expression levels of FOXP3 for cell proliferation and Capase-3 for Apoptosis were determined by qRT-PCR and Western blot analysis. Wound healing model was applied to determine metastasis. The results were evaluated with one-way ANOVA in SPSS 20.0 program as p≤0.05.
Results: It was determined that rosmarinic acid alone and in combination with doxorubicin inhibited the proliferation of OVCAR3 cells in a time- and dose-dependent manner for 24, 48 and 72 hours, and caused the cells to die by causing them to undergo apoptosis. It was observed that Caspase-3 expression increased approximately tenfold in OVCAR3 cells, while FOXP3 expression increased only in RA treatment and was downregulated in DX and RA+DX treatments.
Coclusion According to the results of our study, it was observed that many steps of the signaling pathways related to proliferation, migration and apoptosis were affected by the combination of RA and DX in OVCAR3 cells. This shows that RA will gain an important place in cancer treatment with more comprehensive studies.
Medicine and Pharmacology, Obstetrics and Gynaecology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
