Preprint Communication Version 1 This version is not peer-reviewed

Cytoprotective Effect of Copaifera reticulata Ducke Oil-Resin Extracted from Amazon Flora on Neural Cells Exposed to Rotenone

Gabriel Mesquita da Conceição Bahia
,
Marcio Gonçalves Correa
,
Rebeca da Costa Gomes
,
Thais Alves Lobão
,
Rafael Dias de Souza
,
Elizabeth Sumi Yamada
,
Edmar Tavares Costa
,
Dielly Catrina Favacho Lopes
,
Arnaldo Jorge Martins-Filho
,
Walace Gomes-Leal
,
Anderson Manoel Herculano Oliveira Da-Silva
,
Karen Renata Herculano Matos-Oliveira
,
Carlomagno Pacheco Bahia
*
Version 1 : Received: 30 July 2024 / Approved: 31 July 2024 / Online: 31 July 2024 (10:23:49 CEST)

How to cite: Bahia, G. M. D. C.; Correa, M. G.; Gomes, R. D. C.; Lobão, T. A.; de Souza, R. D.; Yamada, E. S.; Costa, E. T.; Lopes, D. C. F.; Martins-Filho, A. J.; Gomes-Leal, W.; Da-Silva, A. M. H. O.; Matos-Oliveira, K. R. H.; Bahia, C. P. Cytoprotective Effect of Copaifera reticulata Ducke Oil-Resin Extracted from Amazon Flora on Neural Cells Exposed to Rotenone. Preprints 2024, 2024072510. https://doi.org/10.20944/preprints202407.2510.v1 Bahia, G. M. D. C.; Correa, M. G.; Gomes, R. D. C.; Lobão, T. A.; de Souza, R. D.; Yamada, E. S.; Costa, E. T.; Lopes, D. C. F.; Martins-Filho, A. J.; Gomes-Leal, W.; Da-Silva, A. M. H. O.; Matos-Oliveira, K. R. H.; Bahia, C. P. Cytoprotective Effect of Copaifera reticulata Ducke Oil-Resin Extracted from Amazon Flora on Neural Cells Exposed to Rotenone. Preprints 2024, 2024072510. https://doi.org/10.20944/preprints202407.2510.v1

Abstract

The use of pesticides such as rotenone can lead to toxicity, dysfunction and neuronal death in humans. The Copaifera Reticulata Ducke Oil-Resin (CPOR) is widely used by the natives of the Amazon, however, studies on its potential neuroprotective effect are lacking. This study investigated the cytoprotective effect of CPOR on total cell cultures of the ventral midbrain (VMC), hippocampus (HC) and cerebral cortex (CCC) exposed to rotenone at different concentrations. The total cell cultures of VMC, HC and CCC were exposed for 72 or 24 hours to rotenone, CPOR and rotenone + CPOR. Cell viability was measured using the MTT test and compared using one-way ANOVA. Exposure to rotenone reduced the cell viability of CMV, HC and CCC. Exposure to CPOR reduced the cell viability of CMV. The cell viability of CMV exposed simultaneously to rotenone and CPOR was higher compared to rotenone alone. The cell viability of HC exposed simultaneously to rotenone and CPOR was higher than that exposed to rotenone. CMVs are more vulnerable to rotenone toxicity and more sensitive to CPOR. Considering simultaneous exposure, CPOR revealed a possible neuroprotective effect against rotenone damage only for VMC and HC.

Keywords

Central nervous system injuries; Amazon; Natural extracts; Copaifera reticulata; Neurotoxicity

Subject

Biology and Life Sciences, Neuroscience and Neurology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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