Preprint Article Version 1 This version is not peer-reviewed

Dynamics of Mammarenavirus Wenzhouense Acute Infection in the Rodent Rattus norvegicus

ShanShan Du
,
XueFei Deng
,
Xiaoxia Huang
,
TieZhu Liu
,
Aqian Li
,
Qin Wang
,
TingTing Tian
,
Chuan Li
,
ZhangQi Zheng
,
QiHan Lin
,
ZhuoWei Li
,
Shiwen Wang
,
Jiandong Li
*
Version 1 : Received: 31 July 2024 / Approved: 31 July 2024 / Online: 31 July 2024 (15:21:13 CEST)

How to cite: Du, S.; Deng, X.; Huang, X.; Liu, T.; Li, A.; Wang, Q.; Tian, T.; Li, C.; Zheng, Z.; Lin, Q.; Li, Z.; Wang, S.; Li, J. Dynamics of Mammarenavirus Wenzhouense Acute Infection in the Rodent Rattus norvegicus. Preprints 2024, 2024072546. https://doi.org/10.20944/preprints202407.2546.v1 Du, S.; Deng, X.; Huang, X.; Liu, T.; Li, A.; Wang, Q.; Tian, T.; Li, C.; Zheng, Z.; Lin, Q.; Li, Z.; Wang, S.; Li, J. Dynamics of Mammarenavirus Wenzhouense Acute Infection in the Rodent Rattus norvegicus. Preprints 2024, 2024072546. https://doi.org/10.20944/preprints202407.2546.v1

Abstract

Mammarenavirus Wenzhouense (WENV) is broadly distributed in Asia, while the he dynamics process of WENV infection remains unclear. A field derived strain of WENV was used to inoculate Sprague-Dawley (SD) rats by intramuscular injection, the process of virus infection was de-tected by controlling time for 28 days. Viral RNA became detectable in the blood at 3 d pi, and remained detectable for about 12 d. In most organ tissues, viral RNA peaked at 7 d pi, then began to decline by 14 d, however remained detectable in intestine and brain tissues at 21 and 28 d pi. Virus shedding had been detected from fecal samples for 5 days from 6 to 11 d pi using qRT-PCR, and was recovered from feces collected at 8 d pi. Horizontal contact infection occurred among cage-mates at 14 and 21 d pi. Antibodies against the nucleocapsid were detected at 5 dpi, next increased and persisted until the end of experiment. The results enabled to discriminate the kinetic of viremic response, virus shedding in feces and horizontal transmission, as well as the potential sites for WENV replication or maintenance of the virus in nature.

Keywords

Wenzhou virus; acute infection; virus shedding, transmission; rodents; arenavirus.

Subject

Public Health and Healthcare, Other

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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