PreprintArticleVersion 1This version is not peer-reviewed
Investigation of Mutations in the crt-o and mdr1 Genes of Plasmodium vivax for the Molecular Surveillance of Chloroquine Resistance in Parasites from Gold Mining Areas in Roraima, Brazil
Version 1
: Received: 31 July 2024 / Approved: 31 July 2024 / Online: 2 August 2024 (11:45:53 CEST)
How to cite:
Aguiar-Barros, J. D.; Granja, F.; Abreu-Fernandes, R. D.; de Queiroz, L. T.; e Silva, D. D. S.; Citó, A. C.; Mocelin, N. K. A.-D.-O.; Daniel-Ribeiro, C. T.; Ferreira-da-Cruz, M. D. F. Investigation of Mutations in the crt-o and mdr1 Genes of Plasmodium vivax for the Molecular Surveillance of Chloroquine Resistance in Parasites from Gold Mining Areas in Roraima, Brazil. Preprints2024, 2024072582. https://doi.org/10.20944/preprints202407.2582.v1
Aguiar-Barros, J. D.; Granja, F.; Abreu-Fernandes, R. D.; de Queiroz, L. T.; e Silva, D. D. S.; Citó, A. C.; Mocelin, N. K. A.-D.-O.; Daniel-Ribeiro, C. T.; Ferreira-da-Cruz, M. D. F. Investigation of Mutations in the crt-o and mdr1 Genes of Plasmodium vivax for the Molecular Surveillance of Chloroquine Resistance in Parasites from Gold Mining Areas in Roraima, Brazil. Preprints 2024, 2024072582. https://doi.org/10.20944/preprints202407.2582.v1
Aguiar-Barros, J. D.; Granja, F.; Abreu-Fernandes, R. D.; de Queiroz, L. T.; e Silva, D. D. S.; Citó, A. C.; Mocelin, N. K. A.-D.-O.; Daniel-Ribeiro, C. T.; Ferreira-da-Cruz, M. D. F. Investigation of Mutations in the crt-o and mdr1 Genes of Plasmodium vivax for the Molecular Surveillance of Chloroquine Resistance in Parasites from Gold Mining Areas in Roraima, Brazil. Preprints2024, 2024072582. https://doi.org/10.20944/preprints202407.2582.v1
APA Style
Aguiar-Barros, J. D., Granja, F., Abreu-Fernandes, R. D., de Queiroz, L. T., e Silva, D. D. S., Citó, A. C., Mocelin, N. K. A. D. O., Daniel-Ribeiro, C. T., & Ferreira-da-Cruz, M. D. F. (2024). Investigation of Mutations in the <em>crt-o</em> and <em>mdr1</em> Genes of <em>Plasmodium vivax</em> for the Molecular Surveillance of Chloroquine Resistance in Parasites from Gold Mining Areas in Roraima, Brazil. Preprints. https://doi.org/10.20944/preprints202407.2582.v1
Chicago/Turabian Style
Aguiar-Barros, J. D., Cláudio Tadeu Daniel-Ribeiro and Maria de Fátima Ferreira-da-Cruz. 2024 "Investigation of Mutations in the <em>crt-o</em> and <em>mdr1</em> Genes of <em>Plasmodium vivax</em> for the Molecular Surveillance of Chloroquine Resistance in Parasites from Gold Mining Areas in Roraima, Brazil" Preprints. https://doi.org/10.20944/preprints202407.2582.v1
Abstract
(1) Background: Plasmodium vivax causes the largest malaria burden in Brazil, and chloroquine resistance poses a challenge to eliminating malaria by 2035. Illegal mining in the Roraima Yanomami indigenous territory can lead to the introduction of resistant parasites. This study aimed to investigate mutations in the pvcrt-o and pvmdr-1 genes to determine their potential as predictors of P. vivax chloroquine-resistant phenotypes. (2) Methods: Samples were collected in two health centers of Boa Vista. A questionnaire was completed, and blood was drawn from each patient. Then, DNA extraction, PCR, amplicon purification, and DNA sequencing were performed. After alignment with the Sal-1, the amplified fragment was analyzed. Patients infected with the mutant parasites were queried in the Surveillance Information System. (3) Results: 98% (157/164) of participants were from illegal mining areas. The pvcrt-o was sequenced in 151 samples, and the K10 insertion was identified in 13% of them. The pvmdr1 was sequenced in 80 samples: the MYF haplotype (958M) was detected in 92% of them, the TYF in 8%, while MYL was absent. No cases of recrudescence, hospitalization, or death were found. (4) Conclusions: Mutations in the pvcrt-o and pvmdr-1 genes have no potential to predict chloroquine resistance in P. vivax.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
