PreprintArticleVersion 1This version is not peer-reviewed
Unlocking the Puzzle: Investigating the Role of Interleukin 17 Genetic Polymorphisms, Circulating Lymphocytes, and Serum Levels in Venezuelan Women with Recurrent Pregnancy Loss
Version 1
: Received: 5 August 2024 / Approved: 5 August 2024 / Online: 6 August 2024 (02:58:13 CEST)
How to cite:
Garmendia, J. V.; Blanca, I.; Peña, M. J.; De Sanctis, C. V.; De Sanctis, J. B. Unlocking the Puzzle: Investigating the Role of Interleukin 17 Genetic Polymorphisms, Circulating Lymphocytes, and Serum Levels in Venezuelan Women with Recurrent Pregnancy Loss. Preprints2024, 2024080299. https://doi.org/10.20944/preprints202408.0299.v1
Garmendia, J. V.; Blanca, I.; Peña, M. J.; De Sanctis, C. V.; De Sanctis, J. B. Unlocking the Puzzle: Investigating the Role of Interleukin 17 Genetic Polymorphisms, Circulating Lymphocytes, and Serum Levels in Venezuelan Women with Recurrent Pregnancy Loss. Preprints 2024, 2024080299. https://doi.org/10.20944/preprints202408.0299.v1
Garmendia, J. V.; Blanca, I.; Peña, M. J.; De Sanctis, C. V.; De Sanctis, J. B. Unlocking the Puzzle: Investigating the Role of Interleukin 17 Genetic Polymorphisms, Circulating Lymphocytes, and Serum Levels in Venezuelan Women with Recurrent Pregnancy Loss. Preprints2024, 2024080299. https://doi.org/10.20944/preprints202408.0299.v1
APA Style
Garmendia, J. V., Blanca, I., Peña, M. J., De Sanctis, C. V., & De Sanctis, J. B. (2024). Unlocking the Puzzle: Investigating the Role of Interleukin 17 Genetic Polymorphisms, Circulating Lymphocytes, and Serum Levels in Venezuelan Women with Recurrent Pregnancy Loss. Preprints. https://doi.org/10.20944/preprints202408.0299.v1
Chicago/Turabian Style
Garmendia, J. V., Claudia Valentina De Sanctis and Juan Bautista De Sanctis. 2024 "Unlocking the Puzzle: Investigating the Role of Interleukin 17 Genetic Polymorphisms, Circulating Lymphocytes, and Serum Levels in Venezuelan Women with Recurrent Pregnancy Loss" Preprints. https://doi.org/10.20944/preprints202408.0299.v1
Abstract
The immune response is crucial in Pregnancy Loss (RPL), which affects 1-2% of women. In RPL, peripheral blood lymphocytes and local immune cells are activated with a concomitant decrease in the leukocyte tolerogenic response in the uterus and decidua. IL-17 plays an essential role in T cell activation, weakening tolerogenic responses of the T cells. The report aims to analyze the IL-17 genetic polymorphism, circulating IL-17 lymphocyte populations, the reaction upon lymphocyte priming, the amount of cytokine in serum, and the number of T-reg cells in a group of 50 RPL and 50 normal women from the admixed Venezuelan population. No differences were observed in the genetic polymorphisms rs2275913 and rs763780. A significant decrease (P<0.001) was observed in RPL patients compared to controls in the IL-17+ cell populations CD3+, CD8+, CD56+, and CD4CD25 FoxP3, but not in the CD4IL17+ cells. The response upon cell priming was significantly decreased (P<0.001) in all subpopulations of RPL as compared to controls. It is concluded that despite the increase in IL-17A in the serum of patients, the number of circulating IL-17 cells and the lymphocyte response to stimulation was significantly decreased (p<0.001). No differences in the genetic polymorphism compared to controls suggest that biological factors influence IL-17 levels in RPL patients, which must be carefully studied.
Keywords
Recurrent pregnancy loss, IL-17; Th17; genetic polymorphism; T cell activation; T regulatory cells
Subject
Biology and Life Sciences, Immunology and Microbiology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
