Ding, Y.; Lu, G.; Zhang, Y.; Zhang, J.; Ma, J.; Zhao, Y.; Yuan, Y.; Liu, B.; Liu, W.; Shen, W. Effect of EDA1 Variants on Human Pulp Stem Cell Biology and Odontogenic Differentiation. Preprints2024, 2024080373. https://doi.org/10.20944/preprints202408.0373.v1
APA Style
Ding, Y., Lu, G., Zhang, Y., Zhang, J., Ma, J., Zhao, Y., Yuan, Y., Liu, B., Liu, W., & Shen, W. (2024). Effect of EDA1 Variants on Human Pulp Stem Cell Biology and Odontogenic Differentiation. Preprints. https://doi.org/10.20944/preprints202408.0373.v1
Chicago/Turabian Style
Ding, Y., Wei Liu and Wenjing Shen. 2024 "Effect of EDA1 Variants on Human Pulp Stem Cell Biology and Odontogenic Differentiation" Preprints. https://doi.org/10.20944/preprints202408.0373.v1
Abstract
Background: Two explored EDA1 variants regulate the biological behaviors of human dental pulp stem cells (hDPSCs), such as proliferation and migration, as well as their effects on odontogenic differentiation, providing a theoretical basis for further exploration of the mechanisms underlying EDA1-induced tooth development.
Methods: The experimental groups included a wild-type EDA1 group (Wt), non-syndromic tooth agenesis variants EDA1 group (NSTA-A259E, NSTA-S374R) , syndrome type variant EDA1 group (STA-H252L), and an empty vector control group (PCR3/NC). CCK-8 method was used to investigate the proliferative activity of hDPSCs. The cell cycle distribution of hDPSCs was detected by flow cytometry; The cell migration ability was assessed using the cell scratch and Transwell tests; Odontogenic differentiation medium was used to induce mineralization of hDPSCs, followed by statistical analysis. RNA-Seq revealed that FOS, JUN and other genes were found to be enriched in MAPK signaling pathway and osteoclast differentiation pathway. qPCR was used to detect mRNA expression of hub genes (FOS and JUN).
Results: Compared with the NSTA-EDA1 and STA-EDA1, Wt-EDA1 promotes the proliferation and transformation of hDPSCs from the G0/G1 phase to S phase (p0.05). Wt-EDA1 also upregulated hDPSCs cell migration compared to NSTA-EDA1 and STA-EDA variants, as demonstrated by migration assays (p
Keywords
Ectodysplasin-A1; proliferation; migration; odontogenic differentiation; human dulp stem cells; c-FOS
Subject
Medicine and Pharmacology, Dentistry and Oral Surgery
Copyright:
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