Version 1
: Received: 7 August 2024 / Approved: 7 August 2024 / Online: 8 August 2024 (12:09:22 CEST)
How to cite:
Adefiranye, A. O.; Ogunkanmi, L. A.; Adeyemo, O. A.; Shonde Olatunde, T. E.; Paliwal, R.; Abberton, M. T.; Oyatomi, O. A. Analysis of Parents and F1 Progenies Verification in African Yam Bean (Sphenostylis stenocarpa Hochst. Ex. A. Rich.) Harms Using Cowpea SSR Markers.. Preprints2024, 2024080571. https://doi.org/10.20944/preprints202408.0571.v1
Adefiranye, A. O.; Ogunkanmi, L. A.; Adeyemo, O. A.; Shonde Olatunde, T. E.; Paliwal, R.; Abberton, M. T.; Oyatomi, O. A. Analysis of Parents and F1 Progenies Verification in African Yam Bean (Sphenostylis stenocarpa Hochst. Ex. A. Rich.) Harms Using Cowpea SSR Markers.. Preprints 2024, 2024080571. https://doi.org/10.20944/preprints202408.0571.v1
Adefiranye, A. O.; Ogunkanmi, L. A.; Adeyemo, O. A.; Shonde Olatunde, T. E.; Paliwal, R.; Abberton, M. T.; Oyatomi, O. A. Analysis of Parents and F1 Progenies Verification in African Yam Bean (Sphenostylis stenocarpa Hochst. Ex. A. Rich.) Harms Using Cowpea SSR Markers.. Preprints2024, 2024080571. https://doi.org/10.20944/preprints202408.0571.v1
APA Style
Adefiranye, A. O., Ogunkanmi, L. A., Adeyemo, O. A., Shonde Olatunde, T. E., Paliwal, R., Abberton, M. T., & Oyatomi, O. A. (2024). Analysis of Parents and F1 Progenies Verification in African Yam Bean (Sphenostylis stenocarpa Hochst. Ex. A. Rich.) Harms Using Cowpea SSR Markers.. Preprints. https://doi.org/10.20944/preprints202408.0571.v1
Chicago/Turabian Style
Adefiranye, A. O., Michael T. Abberton and Olaniyi A. Oyatomi. 2024 "Analysis of Parents and F1 Progenies Verification in African Yam Bean (Sphenostylis stenocarpa Hochst. Ex. A. Rich.) Harms Using Cowpea SSR Markers." Preprints. https://doi.org/10.20944/preprints202408.0571.v1
Abstract
African yam bean (Sphenostylis stenocarpa Hochst, Ex. A. Rich. Harms) is an important grain legume in sub-Saharan Africa because of its nutritional value and adaptability to various agroecological zones. To foster the varietal development of improved African yam bean (AYB) genotypes with economic traits, it is necessary to validate parental polymorphism for key markers in selecting progenies from crosses between desired parents. The study aims to analyze the genetic fidelity between parents and F1 progenies in African yam bean through putative cowpea Simple sequence repeat (SSR) markers. Hence, a total of seventy-seven progenies were derived from four sets of biparental crossings using drought-susceptible (TSs-96, TSs-363, and TSs-274) and drought-tolerant (TSs-417, TSs-111, and TSs-78) AYB accessions. These were validated using 120 cowpea primers targeting SSR under optimal PCR conditions, and the size of the PCR-amplified DNA fragments was checked using gel electrophoresis. Twenty primers exhibited polymorphism in the parental lines, while ten displayed higher levels of the same polymorphism. The average polymorphism level for the surveyed SSR markers was 0.36. Given these findings, our study demonstrates that cowpea SSR markers are a reliable method for regular testing and clear identification of AYB crosses, indicating potential AYB improvements.
Biology and Life Sciences, Agricultural Science and Agronomy
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
