Version 1
: Received: 7 August 2024 / Approved: 8 August 2024 / Online: 9 August 2024 (10:08:27 CEST)
How to cite:
Eser, E.; Felton, V. A. A.; Drolia, R.; Bhunia, A. K. Salmonella Detection in Food Using a HEK-hTLR5 Reporter Cell-based Sensor. Preprints2024, 2024080647. https://doi.org/10.20944/preprints202408.0647.v1
Eser, E.; Felton, V. A. A.; Drolia, R.; Bhunia, A. K. Salmonella Detection in Food Using a HEK-hTLR5 Reporter Cell-based Sensor. Preprints 2024, 2024080647. https://doi.org/10.20944/preprints202408.0647.v1
Eser, E.; Felton, V. A. A.; Drolia, R.; Bhunia, A. K. Salmonella Detection in Food Using a HEK-hTLR5 Reporter Cell-based Sensor. Preprints2024, 2024080647. https://doi.org/10.20944/preprints202408.0647.v1
APA Style
Eser, E., Felton, V. A. A., Drolia, R., & Bhunia, A. K. (2024). Salmonella Detection in Food Using a HEK-hTLR5 Reporter Cell-based Sensor. Preprints. https://doi.org/10.20944/preprints202408.0647.v1
Chicago/Turabian Style
Eser, E., Rishi Drolia and Arun K Bhunia. 2024 "Salmonella Detection in Food Using a HEK-hTLR5 Reporter Cell-based Sensor" Preprints. https://doi.org/10.20944/preprints202408.0647.v1
Abstract
The development of a rapid, sensitive, specific method for detecting foodborne pathogens is paramount in supplying safe food to enhance public health safety. Despite the significant improvement in pathogen detection methods, key issues are still associated with rapid methods, such as distinguishing living cells from dead, pathogenic potential or health risk of the analyte at the time of consumption, detection limit, and sample-to-result. The mammalian cell-based assays analyze pathogens' interaction with the host cells and are responsive to only live pathogens or active toxins. In this study, a human embryonic kidney (HEK293) cell line expressing Toll-Like Receptor 5 (TLR-5) and chromogenic reporter system (HEK dual hTLR5) was used for the detection of viable Salmonella in a 96-well tissue culture plate. This cell line responds to low concentrations of TLR5 agonist flagellin. Stimulation of TLR5 ligand activates nuclear factor-kB (NF-κB) - linked alkaline phosphatase (AP-1) signaling cascade inducing the production of secreted embryonic alkaline phosphatase (SEAP). With the addition of a ρ-nitrophenyl phosphate as a substrate, a colored end product representing a positive signal is quantified. The assay's specificity was validated with the top 20 Salmonella enterica serovars and 19 non-Salmonella spp. The performance of the assay was also validated with spiked food samples. The total detection time (sample-to-result), including shortened pre-enrichment (4 h) and selective enrichment (4 h) steps with artificially inoculated outbreak-implicated food samples (chicken, peanut kernel, peanut butter, black pepper, mayonnaise, and peach) was 15 h when inoculated at 1–100 CFU/25 g sample. These results show the potential of HEK-DualTM hTLR5 cell-based functional biosensors for rapid screening of Salmonella.
Biology and Life Sciences, Food Science and Technology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
