Larson, J.; Sather, B.; Wang, L.; Westrum, J.; Tokmina-Lukaszewska, M.; Pauley, J.; Copié, V.; McDermott, T. R.; Bothner, B. Metalloproteomics Reveals Multi-Level Stress Response in Escherichia Coli When Exposed to Arsenite. Preprints2024, 2024080690. https://doi.org/10.20944/preprints202408.0690.v1
APA Style
Larson, J., Sather, B., Wang, L., Westrum, J., Tokmina-Lukaszewska, M., Pauley, J., Copié, V., McDermott, T. R., & Bothner, B. (2024). Metalloproteomics Reveals Multi-Level Stress Response in <em>Escherichia Coli </em>When Exposed to Arsenite. Preprints. https://doi.org/10.20944/preprints202408.0690.v1
Chicago/Turabian Style
Larson, J., Timothy R McDermott and Brian Bothner. 2024 "Metalloproteomics Reveals Multi-Level Stress Response in <em>Escherichia Coli </em>When Exposed to Arsenite" Preprints. https://doi.org/10.20944/preprints202408.0690.v1
Abstract
The arsRBC operon encodes a 3-protein arsenic resistance system. ArsR regulates the transcription of the operon, while ArsB and ArsC are involved in exporting trivalent arsenic and reducing pentavalent arsenic respectively. Previous research in Agrobacterium tumefaciens 5A demonstrated ArsR has regulatory control over a wide range of metal related proteins and metabolic pathways. We hypothesized that ArsR has broad regulatory control in other Gram-negative bacteria and set out to test this. Here, we use differential proteomics to investigate changes caused by the presence of the arsR gene in human microbiome-relevant Escherichia coli during arsenite (AsIII) exposure. We show that ArsR has broad-ranging impacts such as the expression of TCA cycle enzymes during AsIII stress. Additionally, we found that the Isc [Fe-S] cluster and molybdenum cofactor assembly proteins are upregulated regardless of the presence of ArsR under these same conditions. An important finding from this differential proteomics analysis was the identification of response mechanisms that were strain, ArsR, and arsenic specific, providing new clarity to this complex regulon. Given the widespread occurrence of the arsRBC operon, these findings should have broad applicability across microbial genera, including sensitive environments such as the human gastrointestinal tract.
Biology and Life Sciences, Biochemistry and Molecular Biology
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