Cubillejo, I.; Theis, K. R.; Panzer, J.; Luo, X.; Banerjee, S.; Thummel, R.; Withey, J. H. Vibrio cholerae Gut Colonization of Zebrafish Larvae Induces Differential Behavior Responses. Preprints2024, 2024081167. https://doi.org/10.20944/preprints202408.1167.v1
APA Style
Cubillejo, I., Theis, K. R., Panzer, J., Luo, X., Banerjee, S., Thummel, R., & Withey, J. H. (2024). Vibrio cholerae Gut Colonization of Zebrafish Larvae Induces Differential Behavior Responses. Preprints. https://doi.org/10.20944/preprints202408.1167.v1
Chicago/Turabian Style
Cubillejo, I., Ryan Thummel and Jeffrey H Withey. 2024 "Vibrio cholerae Gut Colonization of Zebrafish Larvae Induces Differential Behavior Responses" Preprints. https://doi.org/10.20944/preprints202408.1167.v1
Abstract
Cholera is a diarrheal disease prevalent in populations without access to clean water. Cholera is caused by Vibrio cholerae, which colonizes the upper small intestine in humans once ingested. A growing number of studies suggests the gut microbiome composition modulates animal behavior. Zebrafish are an established cholera model that can maintain a complex, mature gut microbiome during infection. Larval zebrafish, which have immature gut microbiomes, provide the advantage of high-throughput analyses for established behavioral models. We identified the effects of V. cholerae O1 El Tor C6706 colonization at 5 days post-fertilization (dpf) on larval zebrafish behavior by tracking startle responses at 10 dpf. We also characterized the larval gut microbiome using 16S rRNA sequencing. V. cholerae-infected or uninfected control groups were exposed to either an alternating light/dark stimuli or a single-tap stimulus, and average distance and velocity were tracked. While there was no significant difference in the light/dark trial, we report a significant decrease in distance moved for C6706-colonized larvae during the single-tap trial. This suggests that early V. cholerae colonization of the larval gut microbiome has a dampening effect on sensorimotor function, supporting the idea of a link between the gut microbiome and behavior.
Biology and Life Sciences, Immunology and Microbiology
Copyright:
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