PreprintArticleVersion 1This version is not peer-reviewed
GW0742 Attenuates Behavioral Abnormalities and Gene Expression Changes of Microglial and Astroglial Proteins in Rat Brain in a Lithium-Pilocarpine Model of Temporal Lobe Epilepsy
Version 1
: Received: 15 August 2024 / Approved: 15 August 2024 / Online: 16 August 2024 (09:53:41 CEST)
How to cite:
Zubareva, O. E.; Kharisova, A. R.; Roginskaya, A. I.; Kovalenko, A. A.; Zakharova, M. V.; Schwarz, A. P.; Sinyak, D. S.; Zaitsev, A. V. GW0742 Attenuates Behavioral Abnormalities and Gene Expression Changes of Microglial and Astroglial Proteins in Rat Brain in a Lithium-Pilocarpine Model of Temporal Lobe Epilepsy. Preprints2024, 2024081221. https://doi.org/10.20944/preprints202408.1221.v1
Zubareva, O. E.; Kharisova, A. R.; Roginskaya, A. I.; Kovalenko, A. A.; Zakharova, M. V.; Schwarz, A. P.; Sinyak, D. S.; Zaitsev, A. V. GW0742 Attenuates Behavioral Abnormalities and Gene Expression Changes of Microglial and Astroglial Proteins in Rat Brain in a Lithium-Pilocarpine Model of Temporal Lobe Epilepsy. Preprints 2024, 2024081221. https://doi.org/10.20944/preprints202408.1221.v1
Zubareva, O. E.; Kharisova, A. R.; Roginskaya, A. I.; Kovalenko, A. A.; Zakharova, M. V.; Schwarz, A. P.; Sinyak, D. S.; Zaitsev, A. V. GW0742 Attenuates Behavioral Abnormalities and Gene Expression Changes of Microglial and Astroglial Proteins in Rat Brain in a Lithium-Pilocarpine Model of Temporal Lobe Epilepsy. Preprints2024, 2024081221. https://doi.org/10.20944/preprints202408.1221.v1
APA Style
Zubareva, O. E., Kharisova, A. R., Roginskaya, A. I., Kovalenko, A. A., Zakharova, M. V., Schwarz, A. P., Sinyak, D. S., & Zaitsev, A. V. (2024). GW0742 Attenuates Behavioral Abnormalities and Gene Expression Changes of Microglial and Astroglial Proteins in Rat Brain in a Lithium-Pilocarpine Model of Temporal Lobe Epilepsy. Preprints. https://doi.org/10.20944/preprints202408.1221.v1
Chicago/Turabian Style
Zubareva, O. E., Denis S. Sinyak and Aleksey V. Zaitsev. 2024 "GW0742 Attenuates Behavioral Abnormalities and Gene Expression Changes of Microglial and Astroglial Proteins in Rat Brain in a Lithium-Pilocarpine Model of Temporal Lobe Epilepsy" Preprints. https://doi.org/10.20944/preprints202408.1221.v1
Abstract
The role of astro- and microglial cells in the pathogenesis of epilepsy is currently under active in-vestigation. It is proposed that the activity of these cells may be regulated by agonists of peroxi-some proliferator-activated nuclear receptors (PPARs). The objective of this study was to investi-gate the effects of the PPAR β/δ agonist GW0742 (Fitorine) on the behavior and gene expression of astro- and microglial proteins involved in the regulation of epileptogenesis in rat brain in a lithi-um-pilocarpine model of temporal lobe epilepsy (TLE). A seven-day administration of GW0742 at a dose of 5 mg/kg/day has been shown to suppress TLE-related behavioral hyperactivity in the Open Field test and to attenuate the TLE-induced increase in gene expression and protein levels of astro- and microglial activation markers Gfap and Aif1 in the dorsal hippocampus and temporal cortex. Furthermore, GW0742 reversed the TLE-related increase in Lcn2 gene expression (a marker of the A1 phenotype of astrocytes) in the temporal cortex, the increase in the ratio of Nos2/Arg1 gene expression (markers of the M1/M2 phenotypes of microglia), and the decrease in Arg1 gene ex-pression in the dorsal hippocampus. The results suggest that GW0742 may mitigate behavioral and biochemical abnormalities in a lithium-pilocarpine model of TLE.
Biology and Life Sciences, Neuroscience and Neurology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
