PreprintArticleVersion 1Preserved in Portico This version is not peer-reviewed
Persistent Levels of Anti-SARS-CoV-2 IgM Antibody Measured by an In-house ELISA in a Convalescent Latin Population Positively Correlate with the Neutralizing Percentages to Several Variants of Concern
Version 1
: Received: 15 August 2024 / Approved: 16 August 2024 / Online: 19 August 2024 (04:31:47 CEST)
How to cite:
Espino, A. M.; Armina-Rodriguez, A.; Cardona, P.; Ocasio-Malavé, C.; Alvarez, L.; Sariol, C. A. Persistent Levels of Anti-SARS-CoV-2 IgM Antibody Measured by an In-house ELISA in a Convalescent Latin Population Positively Correlate with the Neutralizing Percentages to Several Variants of Concern. Preprints2024, 2024081226. https://doi.org/10.20944/preprints202408.1226.v1
Espino, A. M.; Armina-Rodriguez, A.; Cardona, P.; Ocasio-Malavé, C.; Alvarez, L.; Sariol, C. A. Persistent Levels of Anti-SARS-CoV-2 IgM Antibody Measured by an In-house ELISA in a Convalescent Latin Population Positively Correlate with the Neutralizing Percentages to Several Variants of Concern. Preprints 2024, 2024081226. https://doi.org/10.20944/preprints202408.1226.v1
Espino, A. M.; Armina-Rodriguez, A.; Cardona, P.; Ocasio-Malavé, C.; Alvarez, L.; Sariol, C. A. Persistent Levels of Anti-SARS-CoV-2 IgM Antibody Measured by an In-house ELISA in a Convalescent Latin Population Positively Correlate with the Neutralizing Percentages to Several Variants of Concern. Preprints2024, 2024081226. https://doi.org/10.20944/preprints202408.1226.v1
APA Style
Espino, A. M., Armina-Rodriguez, A., Cardona, P., Ocasio-Malavé, C., Alvarez, L., & Sariol, C. A. (2024). Persistent Levels of Anti-SARS-CoV-2 IgM Antibody Measured by an In-house ELISA in a Convalescent Latin Population Positively Correlate with the Neutralizing Percentages to Several Variants of Concern. Preprints. https://doi.org/10.20944/preprints202408.1226.v1
Chicago/Turabian Style
Espino, A. M., Laura Alvarez and Carlos A Sariol. 2024 "Persistent Levels of Anti-SARS-CoV-2 IgM Antibody Measured by an In-house ELISA in a Convalescent Latin Population Positively Correlate with the Neutralizing Percentages to Several Variants of Concern" Preprints. https://doi.org/10.20944/preprints202408.1226.v1
Abstract
The coronavirus SARS-CoV-2 is the causative agent for the COVID-19 first registered in Wuhan, China and responsible for more than 6 million deaths worldwide. Currently, RT-PCR is the gold standard method for diagnosing COVID-19. However, serological tests are needed for screening acute disease diagnosis and screening large populations during the COVID-19 outbreak. Herein we described the development of an in-house enzyme-linked immunosorbent assay (ELISA) for detecting levels of anti-Spike-1-RBD IgM antibody in well-defined serum/plasma panel for screening and identifying subjects infected with SARS-CoV-2 in a Latin population. We found that, compared to the RT-PCR as standard method, the in-house CovIgM-ELISA displayed sensitivities of 96.15% and 93.22% for samples collected up to 30 or 60 days after infection, respectively, as well as 95.59% specificity with 97.3% accuracy. The agreement kappa value (κ) of our CovIgM-ELISA was substantial when compared to RT-PCR (κ =0.873) and to SCoV-2 DetectTM IgM ELISA (κ = 0.684). The IgM levels detected in the population positively correlate with the neutralizing activity against the Wild-type, Alpha and Delta variants but failed to neutralize Omicron. These data indicate that our in-house CovIgM-ELISA is a compatible performing assay for the detection of SARS-CoV-2 infection.
Keywords
COVID-19; IgM; indirect-ELISA; neutralizing antibody; Latin; Puerto Rico
Subject
Biology and Life Sciences, Immunology and Microbiology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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