Preprint Article Version 1 This version is not peer-reviewed

R2R3 MYB Transcription Factor GhMYB201 Promotes Cotton Fiber Elongation via Cell Wall Loosening and Very-Long-Chain Fatty Acid Synthesis

Qingwei Suo
,
Nianjuan Fang
,
Jianyan Zeng
,
Fulin Yan
,
Xi Zhu
,
Yi Wang
,
Wanting Yu
,
Junmin Chen
,
Aimin Liang
,
Yaohua Li
,
Jie Kong
,
Yuehua Xiao
*
Version 1 : Received: 18 August 2024 / Approved: 21 August 2024 / Online: 22 August 2024 (04:09:05 CEST)

How to cite: Suo, Q.; Fang, N.; Zeng, J.; Yan, F.; Zhu, X.; Wang, Y.; Yu, W.; Chen, J.; Liang, A.; Li, Y.; Kong, J.; Xiao, Y. R2R3 MYB Transcription Factor GhMYB201 Promotes Cotton Fiber Elongation via Cell Wall Loosening and Very-Long-Chain Fatty Acid Synthesis. Preprints 2024, 2024081531. https://doi.org/10.20944/preprints202408.1531.v1 Suo, Q.; Fang, N.; Zeng, J.; Yan, F.; Zhu, X.; Wang, Y.; Yu, W.; Chen, J.; Liang, A.; Li, Y.; Kong, J.; Xiao, Y. R2R3 MYB Transcription Factor GhMYB201 Promotes Cotton Fiber Elongation via Cell Wall Loosening and Very-Long-Chain Fatty Acid Synthesis. Preprints 2024, 2024081531. https://doi.org/10.20944/preprints202408.1531.v1

Abstract

Cotton fiber is the leading natural textile material, and fiber elongation plays an essential role in the formation of cotton yield and quality. Although a wealth of components in the molecular network controlling cotton fiber elongation have been reported, a lot of players still need to be functionally dissected to understand the regulatory mechanism of fiber elongation comprehensively. In the present study, an R2R3-MYB transcription factor gene, GhMYB201, was characterized and functionally verified via CRISPR/Cas9 mediated gene editing. GhMYB201 was homologous to Arabidopsis AtMYB60, and both coding genes (GhMYB201At and Dt) were preferentially expressed in elongating cotton fibers. Knocking-out of GhMYB201 significantly reduced the rate and duration of fiber elongation, resulting in shorter and coarser mature fibers. It was found that GhMYB201 could bind and activate the transcription of cell wall loosening genes (GhRDLs), and also β-ketoacyl-CoA synthase (GhKCSs) genes to enhance very-long-chain fatty acid (VLCFA) levels in elongating fibers. Taken together, our data demonstrated that the transcription factor GhMYB201s play essential roles in promoting fiber elongation via activating genes related to cell wall loosening and VLCFA biosynthesis.

Keywords

Gossypium hirsutum; R2R3 MYB transcription factor; fiber elongation; cell wall loosening; KCS

Subject

Biology and Life Sciences, Plant Sciences

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Download PDF Download Supplementary

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our Diversity statement.

Leave a public comment
Send a private comment to the author(s)
* All users must log in before leaving a comment
Views 0
Downloads 0
Comments 0
Get PDF Supplementary Files Cite Share 0
Bookmark BibSonomy Mendeley Reddit Delicious
×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.