Version 1
: Received: 21 August 2024 / Approved: 21 August 2024 / Online: 21 August 2024 (11:40:38 CEST)
How to cite:
Markab, O.; Lafi, Z.; Shalan, N.; Al Daghistani, H.; Fahadawi, A.; Madi, R. Advanced Delivery of Curcumin and Nicotinamide: Promising Solutions for Skin Infections and Melanoma. Preprints2024, 2024081541. https://doi.org/10.20944/preprints202408.1541.v1
Markab, O.; Lafi, Z.; Shalan, N.; Al Daghistani, H.; Fahadawi, A.; Madi, R. Advanced Delivery of Curcumin and Nicotinamide: Promising Solutions for Skin Infections and Melanoma. Preprints 2024, 2024081541. https://doi.org/10.20944/preprints202408.1541.v1
Markab, O.; Lafi, Z.; Shalan, N.; Al Daghistani, H.; Fahadawi, A.; Madi, R. Advanced Delivery of Curcumin and Nicotinamide: Promising Solutions for Skin Infections and Melanoma. Preprints2024, 2024081541. https://doi.org/10.20944/preprints202408.1541.v1
APA Style
Markab, O., Lafi, Z., Shalan, N., Al Daghistani, H., Fahadawi, A., & Madi, R. (2024). Advanced Delivery of Curcumin and Nicotinamide: Promising Solutions for Skin Infections and Melanoma. Preprints. https://doi.org/10.20944/preprints202408.1541.v1
Chicago/Turabian Style
Markab, O., Ali Fahadawi and Razan Madi. 2024 "Advanced Delivery of Curcumin and Nicotinamide: Promising Solutions for Skin Infections and Melanoma" Preprints. https://doi.org/10.20944/preprints202408.1541.v1
Abstract
Abstract
Recently many medications are derived from natural substances that have a potential to fight diseases. These bioactive compounds such as curcumin (CUR) and nicotinamide (NIC) have an antioxidant, anti-inflammatory activity and boosting the immune system.
Aim: This study aims to evaluate antibacterial and antimelanoma activity of CUR-NIC combination and their liposomal preparation.
Method: Activity of CUR-NIC was investigated against skin infection bacteria, Staphylococcus aureus and Pseudomonas aeruginosa. The cytotoxicity assay for melanoma B16 cancer cell line using MTT and migration assays.
Results: The CUR and CUR-NIC (1:1) combination and showed an inhibition zone against S. aureus of 18±0.8 mm and MIC 31.25 µg/ml compared to 16 ±0.85 and 62.5 µg/ml, respectively. The cytotoxicity assay for melanoma B16 cancer cell line revealed that the of CUR and NIC combination synergistically reduced cell proliferation with (CI< 1). Indeed, liposomal preparation showed cytotoxic effect of Lip-CUR (IC50= 31.4 ± 3.2), Lip-NIC (IC50=190 ± 7.3) and Lip-CUR-NIC (IC50 8.5 ± 0.3) while CUR showed IC50 of 9.8 ± 2.2µM and NIC (IC50 135.95 ±10.2 µM).
Conclusion: synergistic effect when curcumin and nicotinamide are combined, potentially offering a more effective cancer treatment strategy.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
