Version 1
: Received: 22 August 2024 / Approved: 22 August 2024 / Online: 23 August 2024 (08:36:00 CEST)
How to cite:
Nackenhorst, M. C.; Hummel, A.; Koeller, M. C.; Gollackner, B.; Regele, H. Fast Green FCF Improves Depiction of Extracellular Matrix in Ex Vivo Fluorescence Confocal Microscopy. Preprints2024, 2024081670. https://doi.org/10.20944/preprints202408.1670.v1
Nackenhorst, M. C.; Hummel, A.; Koeller, M. C.; Gollackner, B.; Regele, H. Fast Green FCF Improves Depiction of Extracellular Matrix in Ex Vivo Fluorescence Confocal Microscopy. Preprints 2024, 2024081670. https://doi.org/10.20944/preprints202408.1670.v1
Nackenhorst, M. C.; Hummel, A.; Koeller, M. C.; Gollackner, B.; Regele, H. Fast Green FCF Improves Depiction of Extracellular Matrix in Ex Vivo Fluorescence Confocal Microscopy. Preprints2024, 2024081670. https://doi.org/10.20944/preprints202408.1670.v1
APA Style
Nackenhorst, M. C., Hummel, A., Koeller, M. C., Gollackner, B., & Regele, H. (2024). Fast Green FCF Improves Depiction of Extracellular Matrix in Ex Vivo Fluorescence Confocal Microscopy. Preprints. https://doi.org/10.20944/preprints202408.1670.v1
Chicago/Turabian Style
Nackenhorst, M. C., Bernd Gollackner and Heinz Regele. 2024 "Fast Green FCF Improves Depiction of Extracellular Matrix in Ex Vivo Fluorescence Confocal Microscopy" Preprints. https://doi.org/10.20944/preprints202408.1670.v1
Abstract
Rapid microscopic analysis of tissue is an essential diagnostic tool in oncological surgery. The gold standard for intraoperative histological tissue evaluation are frozen sections. However, frozen sections are prone to a variety of artefacts and require skilled staff and specialized lab equipment. A potential method for rapid intraoperative tissue evaluation that does not require fixation, freezing nor sectioning of the tissue, is ex vivo fluorescence confocal microscopy (FCM). The visualization of the structurally important extracellular matrix (ECM) in conventional ex vivo FCM lags behind the standards of conventional histology. The objective of this study was to find a stain that will improve the depiction of the ECM to resemble FFPE H&E sections as closely as possible.
Keywords
Digital Pathology; Ex vivo fluorescence confocal microscopy; Extracellular matrix; Frozen sections
Subject
Medicine and Pharmacology, Pathology and Pathobiology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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