PreprintArticleVersion 1Preserved in Portico This version is not peer-reviewed
Insight into Romanian Wild-Grown Heracleum sphondylium: Development of a New Phytocarrier Based on Silver Nanoparticles with Antioxidant, Antimicrobial and Cytotoxicity Potential
Version 1
: Received: 22 August 2024 / Approved: 22 August 2024 / Online: 27 August 2024 (02:12:13 CEST)
How to cite:
Segneanu, A.-E.; Vlase, G.; Vlase, T.; Bejenaru, L. E.; Mogoşanu, G. D.; Buema, G.; Ciocîlteu, M. V.; Bejenaru, C. Insight into Romanian Wild-Grown Heracleum sphondylium: Development of a New Phytocarrier Based on Silver Nanoparticles with Antioxidant, Antimicrobial and Cytotoxicity Potential. Preprints2024, 2024081706. https://doi.org/10.20944/preprints202408.1706.v1
Segneanu, A.-E.; Vlase, G.; Vlase, T.; Bejenaru, L. E.; Mogoşanu, G. D.; Buema, G.; Ciocîlteu, M. V.; Bejenaru, C. Insight into Romanian Wild-Grown Heracleum sphondylium: Development of a New Phytocarrier Based on Silver Nanoparticles with Antioxidant, Antimicrobial and Cytotoxicity Potential. Preprints 2024, 2024081706. https://doi.org/10.20944/preprints202408.1706.v1
Segneanu, A.-E.; Vlase, G.; Vlase, T.; Bejenaru, L. E.; Mogoşanu, G. D.; Buema, G.; Ciocîlteu, M. V.; Bejenaru, C. Insight into Romanian Wild-Grown Heracleum sphondylium: Development of a New Phytocarrier Based on Silver Nanoparticles with Antioxidant, Antimicrobial and Cytotoxicity Potential. Preprints2024, 2024081706. https://doi.org/10.20944/preprints202408.1706.v1
APA Style
Segneanu, A. E., Vlase, G., Vlase, T., Bejenaru, L. E., Mogoşanu, G. D., Buema, G., Ciocîlteu, M. V., & Bejenaru, C. (2024). Insight into Romanian Wild-Grown <i>Heracleum sphondylium</i>: Development of a New Phytocarrier Based on Silver Nanoparticles with Antioxidant, Antimicrobial and Cytotoxicity Potential. Preprints. https://doi.org/10.20944/preprints202408.1706.v1
Chicago/Turabian Style
Segneanu, A., Maria Viorica Ciocîlteu and Cornelia Bejenaru. 2024 "Insight into Romanian Wild-Grown <i>Heracleum sphondylium</i>: Development of a New Phytocarrier Based on Silver Nanoparticles with Antioxidant, Antimicrobial and Cytotoxicity Potential" Preprints. https://doi.org/10.20944/preprints202408.1706.v1
Abstract
Background: Heracleum sphondylium, a medicinal plant used in Romanian ethnopharmacology, proved to have a remarkable biological activity. The escalating concerns surrounding antimicrobial resistance led to a special attention to new efficient antimicrobial agents based on medicinal plants and nanotechnology. We report the preparation of a novel, simple phytocarrier that harnesses the bioactive properties of H. sphondylium and silver nanoparticles (HS-Ag system). Methods: The H. sphondylium low-metabolic profile was determined through gas chromatography–mass spectrometry and electrospray ionization–quadrupole time-of-flight–mass spectrometry. The morphostructural properties of the innovative phytocarrier were analyzed by X-ray diffraction, Fourier-transform infrared spectroscopy, Raman spectroscopy, dynamic light scattering, scanning electron microscopy, and energy-dispersive X-ray spectroscopy. The antioxidant activity was evaluated using total phenolic content, ferric reducing antioxidant power, and 2,2-diphenyl-1-picrylhydrazyl (DPPH) in vitro assays. The antimicrobial activity screening against Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa, and Escherichia coli was conducted using the agar well diffusion method. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay estimated the in vitro potential cytotoxicity on normal human dermal fibroblasts (NHDF) and cervical cancer (HeLa) cells. Results: A total of 88 biomolecules were detected, such as terpenoids, flavonoids, phenolic acids, coumarins, phenylpropanoids, iridoids, amino acids, phytosterols, fatty acids. HS-Ag phytocarrier heightened efficacy in suppressing the growth of all tested bacterial strains compared to H. sphondylium and exhibited a significant inhibition of HeLa cell viability. Conclusions: The new HS-Ag phytocarrier system holds promise for a wide range of medical applications. The data confirms the capacity to augment pertinent theoretical understanding in the innovative field of antimicrobial agents.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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