Version 1
: Received: 22 August 2024 / Approved: 23 August 2024 / Online: 23 August 2024 (15:56:30 CEST)
How to cite:
Caixeta, F.; Martins, V. D.; Figueiredo, A. B.; Afonso, L. C. C.; Tieri, P.; Castiglione, F.; Freitas, L. M.; Maioli, T. DIAMOnD Genes Expression in Human Macrophages Infected with Leishmania major. Preprints2024, 2024081761. https://doi.org/10.20944/preprints202408.1761.v1
Caixeta, F.; Martins, V. D.; Figueiredo, A. B.; Afonso, L. C. C.; Tieri, P.; Castiglione, F.; Freitas, L. M.; Maioli, T. DIAMOnD Genes Expression in Human Macrophages Infected with Leishmania major. Preprints 2024, 2024081761. https://doi.org/10.20944/preprints202408.1761.v1
Caixeta, F.; Martins, V. D.; Figueiredo, A. B.; Afonso, L. C. C.; Tieri, P.; Castiglione, F.; Freitas, L. M.; Maioli, T. DIAMOnD Genes Expression in Human Macrophages Infected with Leishmania major. Preprints2024, 2024081761. https://doi.org/10.20944/preprints202408.1761.v1
APA Style
Caixeta, F., Martins, V. D., Figueiredo, A. B., Afonso, L. C. C., Tieri, P., Castiglione, F., Freitas, L. M., & Maioli, T. (2024). DIAMOnD Genes Expression in Human Macrophages Infected with Leishmania major. Preprints. https://doi.org/10.20944/preprints202408.1761.v1
Chicago/Turabian Style
Caixeta, F., Leandro Martins Freitas and Tatiani Maioli. 2024 "DIAMOnD Genes Expression in Human Macrophages Infected with Leishmania major" Preprints. https://doi.org/10.20944/preprints202408.1761.v1
Abstract
Leishmania spp. commonly infects phagocytic cells of the immune system, particularly macrophages, employing various immune evasion strategies that enable their survival by altering the intracellular environment. In mammals, these parasites establish persistent infections by modulating gene expression in macrophages thus interfering with immune signaling and response pathways ultimately creating a favorable environment for the parasite survival and reproduction. In this study, our objective was to use data mining and subsequent filtering techniques to identify the genes that play a crucial role in the infection process of Leishmania spp. We aimed to pinpoint genes that have the potential to influence the progression of Leishmania infection. To achieve this, we exploited prior, curated knowledge from major databases and constructed 16 datasets of human molecular information consisting of coding genes and corresponding proteins. We obtained over 400 proteins, identifying approximately 200 genes. The proteins coded by these genes were subsequently used to build a network of protein-protein interactions, which enabled the identification of key players. Approximately 10% of these genes were then selected for biological validation. THP-1 cells, a line of human macrophages, were infected with L. major in vitro for the validation process. We observed that L. major has the capacity to impact crucial genes involved in the immune response, resulting in macrophage inactivation and creating a conducive environment for the survival of Leishmania parasites
Keywords
Macrophages; Leishmania; Gene Expression
Subject
Biology and Life Sciences, Immunology and Microbiology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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