Version 1
: Received: 28 August 2024 / Approved: 28 August 2024 / Online: 28 August 2024 (10:03:12 CEST)
How to cite:
Farinati, S.; Devillars, A.; Vannozzi, A.; Scariolo, F.; Palumbo, F.; Barcaccia, G. How Helpful May Be a CRISPR/Cas-Based System for Food Traceability?. Preprints2024, 2024082037. https://doi.org/10.20944/preprints202408.2037.v1
Farinati, S.; Devillars, A.; Vannozzi, A.; Scariolo, F.; Palumbo, F.; Barcaccia, G. How Helpful May Be a CRISPR/Cas-Based System for Food Traceability?. Preprints 2024, 2024082037. https://doi.org/10.20944/preprints202408.2037.v1
Farinati, S.; Devillars, A.; Vannozzi, A.; Scariolo, F.; Palumbo, F.; Barcaccia, G. How Helpful May Be a CRISPR/Cas-Based System for Food Traceability?. Preprints2024, 2024082037. https://doi.org/10.20944/preprints202408.2037.v1
APA Style
Farinati, S., Devillars, A., Vannozzi, A., Scariolo, F., Palumbo, F., & Barcaccia, G. (2024). How Helpful May Be a CRISPR/Cas-Based System for Food Traceability?. Preprints. https://doi.org/10.20944/preprints202408.2037.v1
Chicago/Turabian Style
Farinati, S., Fabio Palumbo and Gianni Barcaccia. 2024 "How Helpful May Be a CRISPR/Cas-Based System for Food Traceability?" Preprints. https://doi.org/10.20944/preprints202408.2037.v1
Abstract
Crops obtained by genome editing (GE) technologies represent a notable and disruptive shift from genetically modified or transgenic plants, outpacing public perception and regulatory frame-works. Multiple aspects of GE are contributing to the advancement of modern agriculture, in-fluencing the market structure by introducing specific changes within the existing genetic blue-print of an organism. Generally, the GE toolbox includes distinct technologies able to incorporate site-specific and pre-determined genomic modifications, exploiting well-established genetic modification strategies. For these reasons, GE-derived approaches are considered new plant breeding methods, known as new breeding techniques (NBTs). Particularly, CRISPR/Cas-based technologies represent a considerable improvement over prior techniques, being more precise, accurate and straightforward for targeted gene editing, in a reliable and reproducible way, with numerous applications. CRISPR/Cas system exploitation for non-editing purposes has also been widely documented, ranging from cell imaging to expression regulation and DNA assembly. Recently, CRISPR/Cas systems have been employed for target detection and could be used to ef-ficiently identify multiple organic chemicals. High-sensitivity and high-specificity detection are two main points of the CRISPR/Cas technology, without needing complex expertise or technical pipelines. This manuscript aims to provide an overview of the main advances of CRISPR/Cas-based systems into new frontiers of non-editing. The associated implications (e.g., molecular traceability) and their relative impacts are also presented and discussed.
Keywords
Genome editing; New breeding techniques; Molecular traceability; CRISPR/Cas system
Subject
Biology and Life Sciences, Food Science and Technology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
