Version 1
: Received: 28 August 2024 / Approved: 29 August 2024 / Online: 29 August 2024 (08:30:20 CEST)
How to cite:
Ávila-Hernández, J. G.; Coreño-Alonso, A.; Pantoja-Alonso, M. A.; Córdoba-Andrade, F. J.; Camas-Reyes, A.; Martínez-Antonio, A. Step-by-Step Development of a Recombinase Polymerase Amplification (RPA) Assay for Sex Identification in Papaya. Preprints2024, 2024082108. https://doi.org/10.20944/preprints202408.2108.v1
Ávila-Hernández, J. G.; Coreño-Alonso, A.; Pantoja-Alonso, M. A.; Córdoba-Andrade, F. J.; Camas-Reyes, A.; Martínez-Antonio, A. Step-by-Step Development of a Recombinase Polymerase Amplification (RPA) Assay for Sex Identification in Papaya. Preprints 2024, 2024082108. https://doi.org/10.20944/preprints202408.2108.v1
Ávila-Hernández, J. G.; Coreño-Alonso, A.; Pantoja-Alonso, M. A.; Córdoba-Andrade, F. J.; Camas-Reyes, A.; Martínez-Antonio, A. Step-by-Step Development of a Recombinase Polymerase Amplification (RPA) Assay for Sex Identification in Papaya. Preprints2024, 2024082108. https://doi.org/10.20944/preprints202408.2108.v1
APA Style
Ávila-Hernández, J. G., Coreño-Alonso, A., Pantoja-Alonso, M. A., Córdoba-Andrade, F. J., Camas-Reyes, A., & Martínez-Antonio, A. (2024). Step-by-Step Development of a Recombinase Polymerase Amplification (RPA) Assay for Sex Identification in Papaya. Preprints. https://doi.org/10.20944/preprints202408.2108.v1
Chicago/Turabian Style
Ávila-Hernández, J. G., Alberto Camas-Reyes and Agustino Martínez-Antonio. 2024 "Step-by-Step Development of a Recombinase Polymerase Amplification (RPA) Assay for Sex Identification in Papaya" Preprints. https://doi.org/10.20944/preprints202408.2108.v1
Abstract
Papaya is a globally important crop, with production primarily based on hermaphrodite plants. Papaya has three sex types: male, female, and hermaphrodite, determined by flower morphology until the flowering stage. In this study, a recombinase polymerase amplification (RPA) assay was developed and optimized to identify the three sexes of papaya. Recombinant uvsX, uvsY, gp32, and Bsu DNA polymerase were used to study the effects of temperature, reaction time, and sensitivity conditions for RPA reaction efficiency. The optimal conditions were found to be 41°C and a 30-minute reaction time, allowing the detection of the target sex from specific DNA markers, even when using crude extract. The study shows that RPA could be used for sex determination in papaya, and the findings could contribute to developing a point-of-need strategy due to their sensitivity and specificity.
Biology and Life Sciences, Agricultural Science and Agronomy
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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