Version 1
: Received: 28 August 2024 / Approved: 29 August 2024 / Online: 29 August 2024 (04:55:37 CEST)
How to cite:
Kovalenko, A. A.; Zakharova, M. V.; Schwarz, A. P.; Zubareva, O. E.; Zaitsev, A. V. Identification of Reliable Reference Genes for Use in Gene Expression Studies in Rat Febrile Seizure Model. Preprints2024, 2024082112. https://doi.org/10.20944/preprints202408.2112.v1
Kovalenko, A. A.; Zakharova, M. V.; Schwarz, A. P.; Zubareva, O. E.; Zaitsev, A. V. Identification of Reliable Reference Genes for Use in Gene Expression Studies in Rat Febrile Seizure Model. Preprints 2024, 2024082112. https://doi.org/10.20944/preprints202408.2112.v1
Kovalenko, A. A.; Zakharova, M. V.; Schwarz, A. P.; Zubareva, O. E.; Zaitsev, A. V. Identification of Reliable Reference Genes for Use in Gene Expression Studies in Rat Febrile Seizure Model. Preprints2024, 2024082112. https://doi.org/10.20944/preprints202408.2112.v1
APA Style
Kovalenko, A. A., Zakharova, M. V., Schwarz, A. P., Zubareva, O. E., & Zaitsev, A. V. (2024). Identification of Reliable Reference Genes for Use in Gene Expression Studies in Rat Febrile Seizure Model. Preprints. https://doi.org/10.20944/preprints202408.2112.v1
Chicago/Turabian Style
Kovalenko, A. A., Olga E. Zubareva and Aleksey V. Zaitsev. 2024 "Identification of Reliable Reference Genes for Use in Gene Expression Studies in Rat Febrile Seizure Model" Preprints. https://doi.org/10.20944/preprints202408.2112.v1
Abstract
The study of the pathogenesis of febrile seizures and their consequences frequently necessitates gene expression analysis. The primary methodology employed for such analysis is reverse transcription with quantitative polymerase chain reaction (RT-qPCR). To guarantee the accuracy of data obtained by RT-qPCR, it is crucial to utilize stably expressed reference genes. The objective of this study was to identify the most suitable reference genes for use in the analysis of mRNA production in various brain regions of rats following prolonged neonatal febrile seizures. The expression stability of eight housekeeping genes was evaluated using the online tool RefFinder in the dorsal and ventral hippocampal regions and in the temporal and medial prefrontal cortex of the brain. The Ppia gene exhibited the greatest stability of expression. Conversely, the genes with the least stable expression level are Actb and Ywhaz; thus, it is not recommended to use them for normalization in a febrile seizure model. Additionally, the majority of housekeeping genes demonstrate age-related, region-specific fluctuations. Therefore, it is crucial to employ the appropriate housekeeping genes for each brain structure under investigation when examining the expression dynamics of genes of interest in a febrile seizure model.
Biology and Life Sciences, Neuroscience and Neurology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
,
†
,
