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Combining Ability of Capsicum annuum Hybrid for Antioxi-Dant Activities, Polyphenol Content, α-Glucosidase Inhibitory, Yield, and Yield Components

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29 August 2024

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30 August 2024

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Abstract
Type II diabetes is a major coronavirus disease comorbidity that can be prevented through a diet. Capsicum annuum (chili) consumption is often suggested, and using functional food cultivars is the most effective strategy. However, the general combining ability (GCA), specific combining ability (SCA), and heterotic effect of functional biochemicals (polyphenol content, antioxidant activities, and α-glucosidase inhibitory compounds) remain poorly known in C. annuum. This study aimed to estimate those parameters in C. annuum by using five different genotypes and their hybrid com-binations based on growth characteristics, yield, yield components, and fruit functional biochem-icals. The F1 and F1R progenies were obtained from crosses in a greenhouse and full diallel design. Each parent used in this study had a GCA advantage for each character. The hybrid combination of 074 x 005 and 435 x 367 displayed the best yield results. However, the results indicated the opposite regarding α-glucosidase inhibitory. The heterotic effect of functional biochemicals was observed for traits related to genotypes, polyphenol content, antioxidant activity, α-glucosidase inhibitory compounds, and similar properties related to yield and yield components, indicating their hybrid use in chili production.
Keywords: 
Subject: Biology and Life Sciences  -   Agricultural Science and Agronomy

1. Introduction

Chili is generally known as a food ingredient because of its spiciness, whose levels depend on the species [1]. Five common species are cultivated, including C. annuum (lowest spiciness) [2] and C. chinense (highest spiciness) [3]. Chili spiciness is caused by a secondary metabolite compound called capsaicin and can be quantified using Scoville heat units [4]. Capsaicin is an active component of chili that can irritate and cause a burning sensation in body tissues [5]. Generally, people use chili spiciness in various ways: flavor enhancer [6], self-protection spray [7], cosmetic raw materials [8,9], or for the pharmaceutical development of chili patches [10,11,12]. Recent studies reported that C. annuum functional secondary metabolites are more beneficial for human health than other species [13].
The chili plant’s most commonly used part is its fruit. The chili fruit has various secondary metabolites with high benefits: capsaicin, alkaloids [14], terpenoids [15], steroids [16], saponins [17], vitamin A [18], vitamin C [18], capsanthin [19], zeaxanthin [20], and cryptoxanthin (used as a dye) [20]. Additionally, chili has various macro minerals (iron, potassium, calcium, phosphorus, and niacin), which are absorbed by the body to maintain human health [21]. In medicine, chili capsaicin is used for its an-algesic properties, relieving asthma and skin itchiness. Additionally, recent studies have reported its anticancer, antidiabetic, and antiarthritis activities, and its role as a gastric acid secretion stimulant to prevent digestive system infections.
Generally, secondary metabolites are produced by plants under environmental stress conditions [22]. However, chili possesses basal antioxidant activity and poly-phenol content [22,23]. Antioxidants counteract free radicals in the body [24], and free radical scavenging helps maintain health and prevent degenerative diseases. One such degenerative disease is diabetes mellitus, which chili can potentially help overcome as a functional food.
The World Health Organization aims to decrease the number of patients with diabetes within the next ten years [25]. Chili consumption can help reach this target through its preventive properties. COVID-19 has changed people’s lifestyles world-wide, making them focus primarily on their diet. A healthy lifestyle includes a diet with foods rich in functional biochemical compounds. Consuming functional bio-chemical compounds by eating chili is a precautionary measure and helps reduce the side effects of chemical drugs impacting patients [26]. Our previous hybridization re-search produced a hybrid performance with functional biochemical compounds to help overcome this problem [27]. Additionally, chili has α-glucosidase inhibitory (AGI) compounds, which inhibit blood sugar absorption [27,28,29,30].
Chili’s functional compounds can be improved in various ways. Plant cultivation techniques can significantly affect the content of active compounds in chili due to en-vironmental influences [31]. One solution is to create new varieties through genetic improvement. New superior varieties can be developed conventionally or with bio-technology methods. Hybridization is a conventional method that produces hybrids with favorable combined properties (yield and biochemical content). The obtained hybrid performance information must be supported by combining ability information as the primary consideration in the selection process. Selecting varieties based on the estimation value of combined abilities for targeted traits will facilitate the development of new chili varieties with high yields and functional biochemical compounds. This study aims to determine the estimated value of combining power in polyphenols, an-tioxidant activity, AGI, yield, and yield components in C. annuum.

2. Materials and Methods

2.1. Plant Material

The parent plants used in this study were selected in previous studies [32,33]. Our previous study reported that the IPB breeding laboratory collection genotypes contained polyphenols, antioxidant activity, and α-glucosidase inhibitors. Five genotypes (IPB367, IPB435, IPB005, IPB374, and IPB074) with distinctive values for hybridization activities were selected as parents. IPB367 is an ornamental chili with AGI and high 2,2-diphenyl-1-picrylhydrazyl (DPPH) antioxidant activity. The IPB367 and IPB 435 genotypes have more fruits but with lower weights than other genotypes. Therefore, IPB005, IPB374, and IPB074, which produced a large quantity of chili fruits with high weights, were selected. The hybridization is expected to produce superior progeny regarding two different traits.
Hybrids and reciprocals were produced from hybridization in Bogor, Indonesia, at the IPB Alam Sinarsari greenhouse. The randomized complete block design was used for the full diallel analysis of five parents [27]. Chili was cultivated in a greenhouse under a controlled environment, following chili cultivation procedures.

2.2. Growth, Yield, and Yield Component Evaluation

F1’s and F1R’s fruits of ten sample plants were evaluated; 20 fruits were harvested from each experimental unit of 25 treatments. The following growth characteristics were measured: dichotomous height (DH) from the base of the soil to the first dichotomous branch (with a digital ruler); leaf length (LL) from base to tip (with a digital ruler); leaf width (LW) from the left end to the right end (with a digital ruler); and stem diameter (SD), at 5 cm high from the ground.
Yield components were also measured: fruit length (FL), using a digital ruler; fruit diameter (FD), calculating the average diameter of ten fruits in one plant in the third to fifth harvests (with a digital caliper); fruit thickness (FT), by calculating the average thickness of ten fruits in one plant in the third to fifth harvests (with a digital caliper); fruit weight (FW), by calculating the average weight of ten fruits in one plant in the third to fifth harvest and weighing it (with a digital analytical balance); the number of fruits per plant (NFP), by counting the total fruit number on one plant during the eight harvests; and yield, by calculating the total fruit weight of one plant during the eight harvests and weighing it (with a digital analytical balance).

2.3. Biochemical Evaluation

Perfectly ripe red chilies were used for biochemical measurements. The following biochemical compounds were sequentially measured: total phenolic content (TPC) with gallic acid standard, total flavonoid content (TFC) with quercetin standard, antioxidant activity of DPPH, ferric reduction antioxidant power method (FRAP) with trolox standard, and AGI with acarbose standard. All measurements were performed by ELISA Reader Spectrophotometry at the following wavelengths: 750, 415, 517, 595, and 410 (following the order cited above). The measured absorbance results were converted using standard charts and analyzed to estimate the combining ability and heterotic effect. The extraction was performed using 3 g of chili powder dissolved in 20 mL of 70% ethanol, and samples were left on an orbital shaker for 48 h. Samples were then filtered and deposited in a vial for 24 h in the refrigerator [34].

2.4. Data Statistical Analysis

General combining ability (GCA), specific combining ability (SCA), and heterotic effect of functional compounds were estimated using the full diallel method following Griffing’s methodology [1956; Method I, Model 1 (fixed effect)]. All performance data were analyzed using “Analysis of Genetics Designs” in R (AGD-R) v. 5.0. (Centro Internacional de Mejoramiento de Maíz y Trigo). The raw data were collected from field and laboratory analysis with three replications. Each replication used ten samples for analysis. The statistical model was defined as follows:
Yij = m + gi + gj + sij + rij + 1/bc ΣΣeijkl
where Yij is the mean value of hybrid and parent genotypes; m is the effect of general means; gi and gj are effects of the GCA related to the i-th and j-th parent, respectively; sij is the effect of SCA for the crossings between the parents of order i and j; rij is the reciprocal effect; and 1/bc ΣΣeijkl is the mean’s value of error.
The critical difference test (CD) was conducted to evaluate differences in GCA between parents. If GCA > CD, the GCA was considered significantly different. The heterotic and heterobeltiosis concerning the mean of the parents was calculated for each character, using the AGD-R program:
CD = √(MSe/p) x t (5% table)
where CD is the critical difference test; MSe is the mean square error, and p is the number of parents.

3. Results

3.1. Analysis of Variance

GCA and SCA estimates were essential components obtained from chili plant breeding activities. Variance analysis in this study significantly affects the GCA mean square for growth characters, yield, yield components, and biochemical content in all observed variables. The SCA mean square also significantly affected all observed variables (Table 1, Table 2 and Table 3).
Sources of reciprocal diversity in all variables observed for growth characters, yield, yield components, and biochemical content displayed a significant effect at the 1% level. The significant effect on reciprocity indicated that the F1 and F1 reciprocals tested in this study differed significantly. Significantly different information on the diversity sources in GCA and SCA is the basis for further estimating combining abilities. Thus, the coefficient of variance of diversity in growth characters, yield, and yield components were produced between 1.61%–5.03% and 2.64%–6.81% (Table 1 and Table 2). The coefficient of variance of the variable observed for biochemical content was produced between 1.60%–3.26% (Table 3).

3.2. GCA Analysis

IPB005 was the parent genotype with the highest GCA for several growth characters and yield components, including LW, FD, FT, FW, NFP, and yield. The best GCA for SD, LL, and FL characters was displayed by parent IPB374 (Table 4 and Table 5). IPB074 had the best GCA for DH and TPC. Meanwhile, IPB435 displayed the best GCA for TFC and FRAP and IPB367 for DPPH and AGI.
The GCA results for yield and yield components varied between parents (Table 5). Chili lines IPB005 and IPB374 had positive GCA for FL, FT, FW, and yield. Meanwhile, the chili lines IPB367 and IPB435 had negative GCA for the same characters, especially IPB367, which had the highest negative GCA for FL (GCA −2.86) and NFP (GCA −2.49). The IPB435 parent had the highest negative GCA for FD (−3.14), FW (−3.38), and yield (−238.53).
The highest GCA values for five parents were evenly distributed. Each parent displayed advantages in the desired character. The parents IPB005, IPB367, and IPB074 have positive GCA for DPPH antioxidant activities, while IPB374 and IPB435 exhibited the highest negative GCA for DPPH antioxidant activities. Only parents IPB005 and IPB435 had a positive GCA for FRAP antioxidant activities (0.56 and 4.62, respectively). IPB435 had positive GCA results for AGI (3.99) and TFC (0.35). Meanwhile, IPB435 had a negative GCA for TPC (−0.80). IPB367 displayed a positive GCA on all biochemical observations, including TPC, TFC, DPPH, and AGI, except FRAP antioxidant activity, with respective values of 2.18, 0.18, 0.08, 9.86, and −3.07 (Table 6).

3.3. SCA Analysis

Parents with positive and negative SCA in DH and SD (12 and 11 combinations of crosses) exhibited positive SCA in LL and LW. The highest SCA for DH was displayed by hybrid 374 x 435 (4.33), followed by hybrid 005 x 435 (3.67), 074 x 435 (3.64), and 074 x 374 (3.44). The combined 435 x 374 and 435 x 367 hybrids had a negative SCA for LL (−0.61 and −0.73) and leaf width (−0.47 and −0.37). Positive SCA values for all growth characters were displayed by hybrid 367 x 005, which also had the highest positive SCA values for LL (0.69) (Table 7).
Yield and yield components for SCA estimates included FL, FD, FT, FW, and NFP (Table 8). SCA for FL was between −1.12–+1.12. The lowest and highest SCA for FL were in hybrids 005 x 435 and 005 x 374. The three highest SCA values for FD were seen in hybrids 074 x 005, 367 x 005, and 435 x 367 (2.51, 2.49, and 1.74, respectively). Hybrid 074 x 005 had a negative SCA value (-0.43) for FT.
FW, NFP, and FW per plant were the main characteristics that determined potential chili productivity. The highest SCA values for FW were observed in hybrids 074 x 005, 374 x 005, and 005 x 367. The highest SCA for NFP was observed in hybrids 435 x 374, 074 x 367, and 435 x 005. Meanwhile, the highest SCA for FW per plant was for hybrid 074 x 005, which was recommended for high potential yield (Table 8).
Eight hybrids had positive SCA, and 12 had negative SCA for TPC and AGI characters. SCA for antioxidant activity displayed contradictory results depending on the method used. DPPH had 13 positive SCA in contrast to FRAP, which had 13 negative SCA. The hybrid 367 x 374 had the lowest negative SCA for the three biochemical constituents (TPC: −9.57; TFC: −0.89; FRAP: −12.36). The highest SCA for AGI and TPC were observed for hybrid 074 x 374 (7.83 and 4.06). Hybrid 435 x 367 exhibited the highest positive SCA for TFC (1.11) and FRAP (8.23). Finally, hybrid 367 x 374 had the highest positive SCA for DPPH (0.16) (Table 9).

3.4. Heterotic and Heterobeltiosis Effects in Chili

The results of heterotic and heterobeltiosis effect analysis on growth characters, yield components, and biochemical content are presented in Table 10, Table 11 and Table 12. The heterotic effect for DH was −20.75%–32.95%, whereas the effect of heterobeltiosis was −25.61%–24.24%. The highest heterotic and heterobeltiosis effects for this character were observed in hybrids 435 x 074 and 374 x 074. Hybrid 435 x 374 consistently displayed the second-highest heterotic and heterobeltiosis effects. The highest heterotic effect for leaves (LL and LW) was observed in hybrid 367 x 005; Heterotic and heterobeltiosis effects were 35.98% and 9.36% for LL and 15.46% and −8.94% for LW, respectively. Those effects were the highest for SD in hybrid 435 x 374. The hybrids 367 x 374 and 367 x 435 had the overall lowest heterobeltiosis effect (Table 10).
The highest effects for fruit length (HMP, 4.47%; HHP, −1.83%) were displayed by two different hybrids, 374 x 005 and 435 x 367. FD and FT resulted in heterotic effect values of −33.84%–22.69% and −41.06%–5.19%. Meanwhile, the heterobeltiosis effect for these two characters was −51.88%–−1.79% and −54.48%–-6.79%, with the highest value displayed by 435 x 374 and 074 x 374. The highest heterotic and heterobeltiosis effects for the three characters (FW, NFP, yield) were observed in 435 x 367, 435 x 374, and 005 x 374. The highest heterobeltiosis values differed in FW character for hybrid 005 x 074, which had the highest value. The heterotic effect for FW per plant was −75.44%–15.20% (Table 11).
The heterotic and heterobeltiosis effects for TPC were −56.09%–13.91% and −56.31%–4.21%, respectively. The highest and lowest effects for this character were displayed by hybrid 435 x 074 and 367 x 374, respectively. The best effects for TFC were observed in hybrid 005 x 074, followed by the reciprocal hybrid 074 x 005. Interestingly, the same highest value was observed with the FRAP method. The best effects for DPPH were seen in hybrid 074 x 435. The heterobeltiosis effect for FRAP was negative for all hybrids. The highest effects for AGI were seen in hybrid 005 x 074, followed by the reciprocal hybrid 074 x 374 (Table 12).

4. Discussion

Improving chili varieties through hybridization remains a common practice among chili breeders. Hybridization takes advantage of Mendel’s segregation laws, where genetic carriers of superior traits are exchanged from two parents. The chili breeding milestone begins with improving high productivity and continues with enhancing plant resistance toward pests and diseases. Developing global issues (COVID-19 pose a new challenge for chili breeders to obtain superior varieties as functional foods. This study evaluated five C. annuum parents (self-pollinated) and 20 hybrids and their reciprocals regarding the yield and functional biochemical contents, including polyphenols, antioxidants, and AGI compounds.
The performance of hybrids and parents indicates the success of upgrading a particular character. However, at the genetic level, such performance can be predicted through diallel analysis so that plant breeders can select the best parents for further crosses. The diallel analysis can provide information on the estimated effects of the GCA and SCA. This estimation can identify segregated populations with the potential for high genetic variability and superior hybrid combinations [34,35,36,37]. Information on GCA and SCA estimation is crucial and is obtained by diallel analysis using the Griffing method [38]. Genetic studies using this method reveal information on the magnitude of the GCA effect, which can indicate the superiority of the dominant parent in additive effects [39]. Additionally, the SCA effect can also be estimated, demonstrating the superiority of dominant hybrids in nonadditive results. This analysis can also indicate the expected behavior of the resulting hybrid population based on the parent GCA [40].
Parents with high GCA values can increase the expression of the resulting hybrids. Meanwhile, a low GCA value indicates that the parent does not differ from the average of the entire diallel. A higher GCA value contributes to increasing the expression of the intended trait. A positive or negative GCA value indicates that the parent is superior or inferior, respectively. However, a low GCA is better for some traits, like disease resistance [41], plant height, and canopy width in ornamental plants [42,43]. A less negative value for yield components refers to decreased potential yield [44]. Information on GCA and SCA is used to determine parents and appropriate breeding methods to improve the selected character in plants [45].
GCA estimates are used to determine parents for future research [46]. A parent with high GCA could also be used for synthetic varieties and hybrid development programs [47]. Variety development based on growth characters can use DH, LL, and short LW. In this study, the IPB367 genotype was the best for chili’s ornamental development. In contrast, increased yield and yield components require tall plants, sturdy stems, and broad leaves to maximize photosynthesis and produce large fruits [48].
Variety development programs aiming to increase productivity can rely on parent GCA values (Table 4–6). The highest GCA for yield were observed for IPB005, IPB074, and IPB374. These three genotypes are also recommended to develop new varieties for large fruits. IPB435 had a high GCA for NFP, besides IPB005 and IPB374.
IPB435 had a high combining ability, in contrast to IPB367, for FRAP antioxidants. However, IPB367 had a better GCA than other parents for DPPH and AGI. Therefore, this line is recommended for the pharmaceutical industry, which produces supplements or drugs for diabetes patients. In line with our previous study, these results suggest that parents with high GCA values for capsaicin could be used to develop spicy C. annuum varieties [49].
The SCA effect is a reflection produced by the hybrid based on the GCA value. The hybrid 074 x 005 had the highest SCA for yield resulting from the two parents with the highest positive GCA. Interestingly, for the same character in this study, a hybrid combination with the second highest positive value (hybrid 435 x 367) resulted from the two parents with negative GCA values. Additionally, the SCA value of positive yield characters could be produced from two parents with negative GCA in plant height characters [50]. The AGI displayed the same trend with the highest SCA value displayed by 074 x 374, which was produced from two parents with a negative GCA, including one with the highest negative GCA (IPB074). This is caused by positive genes spreading within the parents and covering the negative genes in the partner parents so they can combine well [51]. A high SCA value indicates better or worse performance than expected, depending on the evaluated characteristics. SCA also estimated the effect of nonadditive and epistatic genes [52].
Diallel analysis can also predict heterotic and heterobeltiosis effects. The heterotic effect indicates that the hybrid performance is better than the means of parents, while the heterobeltiosis effect suggests that the hybrid performance is better than the best parents [53,54,55]. Combinations with the best heterotic and heterobeltiosis effects which poor performance are not easy to use to become hybrid varieties. The highest heterotic effect in leaf (LL and LW) was seen in 367 x 005, which had the highest positive SCA for LL. This result indicates parents with positive and negative GCA produce hybrids with the highest SCA and heterotic effect regarding leaves. A high SCA also results in a high heterotic effect [56]. Meanwhile, heterotic and heterobeltiosis effects for yield were displayed by different combinations (005 x 374 and 005 x 074) with the same female parent (IPB005). This result aligns with our previous research [49] evaluating chili production as a spice with good heterotic effects in the hybrids evaluated.
The highest heterotic and heterobeltiosis effects for TFC, DPPH, and AGI were displayed by 005 x 074, 074 x 114, and 374 x 074. Interestingly, this study’s heterobeltiosis effect for antioxidant FRAP was negative for all hybrids. The heterotic effect is formed by crossing two parents with different gene frequencies [57], which explains that the self-pollination genotype has a zero heterotic effect. Several studies have demonstrated the benefits of using chili hybrids by exploiting the heterotic phenomenon [58].

5. Conclusions

IPB005, IPB074, IPB374, IPB367, and IPB435 had high-affinity potential and advantages as parents based on the observed characters. They can form a superior segregated population determined by the direction of the breeding program. The hybrid 374 x 074 is the best combination considering AGI production; it can be developed for the pharmaceutical industry. Additionally, the hybrid 005 x 074 displayed the best yield. All hybrids observed had a heterotic effect; they could be developed for further research. The GCA and SCA values produced in this study can help breeders develop hybrid chili plants. Moreover, the heterosis effect produced in this study confirmed that hybrid means are higher than that of parents.

Author Contributions

Conceptualization, M.S.Y., A.M.A., and W.N.C.; methodology, A.N.N.; software, A.W.R.; validation, M.S.Y., Z.D.S., and O.S.I.; formal analysis, S.M.A; investigation, M.R.A.; writing—original draft preparation, G.R.P., A.Y.P., and A.N.N.; writing—review and editing, A.H.A., and Z.D.S; visualization, A.W.R., A.M.A., and S.M.A.; supervision, W.N.C., M.S.Y., A.M.A.; project administration, Z.D.S. All authors have made equal contributions to this article. All authors have read and agreed to the published version of the manuscripts.

Funding

This research was funded by Indonesian Ministry of Education and Culture through the Applied Research of National Higher Education in 2024 with Muhamad Syukur as the principal investigator.

Acknowledgments

The authors would like to thank the Ministry of Research, Technology and Higher Education of the Republic of Indonesia, Centre of Tropical Horticulture Studies IPB, National Research and Innovation Agency Indonesia and Institut Pertanian Bogor (IPB University).

Conflicts of Interest

The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results.

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Table 1. ANOVA of chili growth characters.
Table 1. ANOVA of chili growth characters.
Sources df Mean Square
DH LL LW SD
GCA 4 219.98** 10.25** 1.71** 6.85**
SCA 10 15.91** 0.95** 0.25** 3.05**
Reciprocal 10 8.91** 1.01** 0.22** 8.35**
Error 48 0.68 0.03 0.01 0.01
Coefficient of Variance (%) 5.03 3.20 4.86 1.61
df, degree of freedom; GCA, general combining ability; SCA, specific combining ability; DH, dichotomous height; LL, leaf length; LW, leaf width; SD, stem diameter; ** significant at P ≤ 0.01.
Table 2. ANOVA of chili yield and yield components.
Table 2. ANOVA of chili yield and yield components.
Sources df Mean Square
FL FD FT FW NFP Yield
GCA 4 55.76** 85.93** 0.48** 88.41** 39.04** 445617.7**
SCA 10 0.94** 8.05** 0.14** 9.63** 796.63** 35735.5**
Reciprocal 10 0.67** 0.93** 0.06** 0.29** 3.79ns 1008.59**
Error 48 0.03 0.03 0.002 0.05 3.61 307.77
Coefficient of Variance (%) 4.03 2.64 5.03 6.17 4.71 6.81
df, degree of freedom; GCA, general combining ability; SCA, specific combining ability; FL, fruit length; FD, fruit diameter; FT, fruit thickness; FW, fruit weight; NFP, fruit number per plant; ** significant at P ≤ 0.01; ns, not significant.
Table 3. ANOVA of chili biochemical compounds.
Table 3. ANOVA of chili biochemical compounds.
Sources df Mean Square
TPC TFC DPPH FRAP AGI
GCA 4 100.18** 0.66** 0.07** 86.75** 500.27**
SCA 10 129.29** 1.31** 0.04** 150.56** 40.73**
Reciprocal 10 1.71** 0.01** 0.002** 3.39** 0.81**
Error 48 0.26 0.002 0.001 0.42 0.38
Coefficient of Variance (%) 3.16 3.20 1.19 3.26 1.60
df, degree of freedom; GCA, general combining ability; SCA, specific combining ability; TPC, total phenolic content; TFC, total flavonoid content; DPPH, antioxidant activities; FRAP, antioxidant activities; AGI, α-glucosidase inhibitor; ** significant at P ≤ 0.01.
Table 4. GCA for chili growth characters.
Table 4. GCA for chili growth characters.
Genotypes DH LL LW SD
IPB005 -1.27d 0.56b 0.47a 0.15b
IPB374 3.13b 0.77a 0.32b 1.25a
IPB367 -6.94e -1.75e -0.57e 0.06b
IPB435 -0.24c 0.37c -0.21d -0.51c
IPB074 5.33a 0.06d -0.01c -0.95d
Critical difference 0.79 0.16 0.10 0.10
DH, dichotomous height; LL, leaf length; LW, leaf width; SD, stem diameter; Numbers followed by the same letter in the same column are not significantly different according to the critical difference test at 5%.
Table 5. GCA for chili yield and yield components.
Table 5. GCA for chili yield and yield components.
Genotypes FL FD FT FW NFP Yield
IPB005 0.88c 4.47a 0.28a 3.17a 2.14a 239.24a
IPB374 2.57a -0.39c 0.16b 1.10c 0.87a 69.99c
IPB367 -2.86e -1.87d -0.09c -2.93d -2.49b -203.75d
IPB435 -2.11d -3.14e -0.27d -3.38e 1.14a -238.53e
IPB074 1.53b 0.93b -0.08c 2.04b -1.66b 133.05b
Critical difference 0.16 0.16 0.04 0.21 1.81 16.71
FL, fruit length; FD, fruit diameter; FT, fruit thickness; FW, fruit weight; NFP, number of fruits per plant. Numbers followed by the same letter in the same column are not significantly different according to the critical difference test at 5%.
Table 6. GCA for chili biochemical contents.
Table 6. GCA for chili biochemical contents.
Genotype TPC TFC DPPH FRAP AGI
IPB005 -3.08d -0.14c 0.04b 0.56b -3.88d
IPB374 -2.59d -0.26d -0.09c -0.22c -1.65c
IPB367 2.18b 0.18b 0.08a -3.07e 9.86a
IPB435 -0.80c 0.35a -0.09c 4.62a 3.99b
IPB074 4.30a -0.13c 0.05ab -1.89d -8.32e
Critical difference 0.49 0.04 0.03 0.62 0.59
TPC, total phenolic content; TFC, total flavonoid content; DPPH, antioxidant activities; FRAP antioxidant activities; AGI, α-glucosidase inhibitor. Numbers followed by the same letter in the same column are not significantly different according to the critical difference test at 5%.
Table 7. SCA for chili growth characters.
Table 7. SCA for chili growth characters.
Chili Hybrid DH LL LW SD
005 x 374 -0.33 0.33 0.68 -1.24
005 x 367 1.5 0.4 0.001 -3.62
005 x 435 3.67 0.67 -0.12 0.47
005 x 074 -1.00 0.37 0.4 -1.41
374 x 005 1.37 0.68 0.43 -0.78
374 x 367 -1.83 -0.08 0.001 -3.89
374 x 435 4.33 -1.42 -0.42 2.51
374 x 074 -0.67 -1.18 -0.48 1.64
367 x 005 1.61 0.69 0.13 1.67
367 x 374 -0.46 0.004 0.28 -0.34
367 x 435 -0.17 0.55 0.2 0.62
367 x 074 -1.33 0.13 -0.08 0.59
435 x 005 -2.26 0.61 0.181 -0.69
435 x 374 0.67 -0.61 -0.47 1.37
435 x 367 0.24 -0.73 -0.37 -2.03
435 x 074 -1.83 0.68 0.02 -0.25
074 x 005 -2.16 -0.05 -0.202 0.62
074 x 374 3.44 -0.24 -0.27 2.66
074 x 367 1.17 0.42 0.15 -0.06
074 x 435 3.64 -0.04 -0.02 2.46
DH, dichotomous height; LL, leaf length; LW, leaf width; SD, stem diameter.
Table 8. SCA for chili yield and yield components.
Table 8. SCA for chili yield and yield components.
Chili Hybrids FL FD FT FW NFP Yield
005 x 374 1.12 0.61 -0.26 0.75 0.5 -22.82
005 x 367 0.65 0.15 0.05 0.81 0.83 16.51
005 x 435 -1.12 -0.72 -0.34 -0.04 -0.17 -7.38
005 x 074 -0.28 -0.38 -0.01 -0.19 -3.00 -54.32
374 x 005 0.4 0.66 -0.08 1.78 -5.27 100.69
374 x 367 0.23 -0.67 0.32 -0.37 0.001 -0.31
374 x 435 0.12 0.12 0.003 -0.11 0.5 -1.67
374 x 074 0.03 -1.18 0.11 -0.19 -1.5 -34.32
367 x 005 -0.81 2.49 -0.04 -2.34 8.76 -115.88
367 x 374 -0.38 -0.24 -0.19 -0.76 -18.47 -61.09
367 x 435 0.1 0.28 0.013 0.05 -1.33 2.06
367 x 074 0.001 0.09 -0.04 0.1 -0.17 7.695
435 x 005 -0.56 -2.81 -0.03 -3.07 11.46 -102.67
435 x 374 0.02 1.39 -0.27 -0.79 27.39 17.53
435 x 367 0.46 1.74 0.08 2.77 -28.07 104.03
435 x 074 -0.52 1.28 0.09 -0.09 2.17 -0.61
074 x 005 0.01 2.51 -0.43 2.09 0.76 135.85
074 x 374 -0.99 -0.75 0.26 -1.84 5.19 -90.17
074 x 367 -0.31 -2.13 -0.19 -1.53 16.56 -71.09
074 x 435 0.19 -0.82 0.12 -1.16 -30.74 -206.33
FL, fruit length; FD, fruit diameter; FT, fruit thickness; FW, fruit weight; NFP, number of fruits per plant.
Table 9. SCA for chili biochemical compounds.
Table 9. SCA for chili biochemical compounds.
Chili Hybrids TPC TFC DPPH FRAP AGI
005 x 374 0.17 -0.01 0.001 -0.61 -0.22
005 x 367 -0.28 0.05 -0.03 -0.67 -0.5
005 x 435 -0.42 0.06 0.04 -0.46 0.24
005 x 074 -0.69 -0.03 0.04 0.49 -0.88
374 x 005 -3.26 0.08 -0.1 -3.53 -3.09
374 x 367 -1.01 -0.01 -0.01 0.52 -0.15
374 x 435 0.15 0.14 0.04 3.24 -1.03
374 x 074 -1.12 0.07 0.04 -1.12 -0.2
367 x 005 -6.06 -0.73 0.11 -0.92 0.68
367 x 374 -9.57 -0.89 0.16 -12.36 4.4
367 x 435 -0.35 0.02 0.03 0.88 -0.61
367 x 074 0.52 0.02 0.02 1.39 0.76
435 x 005 -7.39 -0.48 0.07 -6.58 2.99
435 x 374 -7.44 -0.47 -0.014 -1.93 -2.8
435 x 367 7.72 1.11 -0.28 8.23 -0.52
435 x 074 -2.26 -0.03 0.03 -0.97 0.91
074 x 005 2.99 0.76 -0.02 5.96 -6.68
074 x 374 4.06 0.26 0.01 -0.27 7.83
074 x 367 1.65 -0.85 -0.03 -10.17 0.23
074 x 435 0.58 -0.64 0.2 -2.89 -0.004
TPC, total phenolic content; TFC, total flavonoid content; DPPH, antioxidant activities DPPH method; FRAP, antioxidant activities FRAP method; AGI, α-glucosidase inhibitory.
Table 10. Heterotic and heterobeltiosis effects for chili growth characters.
Table 10. Heterotic and heterobeltiosis effects for chili growth characters.
Chili Hybrids TD PD LD DBT
HMP HHP HMP HHP HMP HHP HMP HHP
..……….………………………… (%) ..……….…………………………
005 x 374 12.28 7.87 13.55 -0.32 0 -3.05 32.79 31.77
005 x 367 3.39 -25.61 23.28 -0.85 15.46 -8.94 68.68 54.86
005 x 435 -20.75 -23.17 5.93 -6.23 5.79 4.07 -3.2 -14.22
005 x 074 3.87 -5.05 7.26 1.92 -12.3 -13.01 30.44 24.99
374 x 005 9.94 5.62 20.88 6.11 32.28 28.24 -2.13 -2.88
374 x 367 24.8 -12.36 2.64 -25.08 10.89 -14.5 59.42 47.39
374 x 435 0.00 -6.74 3.25 2.25 -9.6 -13.74 13.71 0.09
374 x 074 30.85 24.24 9.44 0.64 0.79 -3.05 30.66 24.28
367 x 005 18.64 -14.63 35.98 9.36 15.46 -8.94 -24.63 -30.81
367 x 374 7.2 -24.72 0.44 -26.69 10.89 -14.5 -40.37 -44.87
367 x 435 15.04 -15.58 -19.64 -40.98 -25.26 -40.34 -35.54 -46.96
367 x 074 30.37 -11.11 7.92 -16.48 5.21 -16.53 -18.87 -28.36
435 x 005 6.92 3.66 20.74 6.89 0 -1.63 11.67 -1.04
435 x 374 31.33 22.47 -24.35 -25.08 -29.6 -32.82 93.29 70.14
435 x 367 13.27 -16.88 -4.91 -30.16 -12.63 -30.25 -17.69 -32.27
435 x 074 32.95 18.18 -11.31 -17.7 -13.33 -14.05 48.25 36.6
074 x 005 -2.76 -11.11 16.13 10.34 7.38 6.5 -11.36 -15.07
074 x 374 26.6 20.2 -15.38 -22.19 -22.22 -25.19 78.63 69.91
074 x 367 18.52 -19.19 11.88 -13.41 0 -20.66 -3.18 -14.49
074 x 435 20.45 7.07 3.18 -4.26 -12.5 -13.22 39.97 28.97
DH, dichotomous height; LL, leaf length; LW, leaf width; SD, stem diameter; HMP, Heterotic mid parent (heterotic effect); HHP, Heterotic high parent (heterobeltiosis effect).
Table 11. Heterotic and heterobeltiosis effects for chili yield and yield components.
Table 11. Heterotic and heterobeltiosis effects for chili yield and yield components.
Chili Hybrids FL FD FT FW NFP Yield
HMP HHP HMP HHP HMP HHP HMP HHP HMP HHP HMP HHP
…………………………………………… (%) ……………………………………………
005 x 374 -13.59 -23.93 -2.94 -30.42 -10.14 -19.01 -4.54 -18.4 10.74 6.91 15.2 -3.06
005 x 367 -35.24 -57.63 -31.14 -48.79 -23.78 -37.53 -60 -76.95 7.16 -10.08 -35.86 -61.46
005 x 435 1.86 -31.78 -23.67 -51.88 -1.53 -29.78 -63.11 -79.4 12.64 -7.97 -33.85 -61.21
005 x 074 -6.57 -12.09 6.46 -12.29 -37.37 -49.27 2.27 -2.71 11.28 -0.89 9 6.85
374 x 005 4.47 -8.03 4.48 -25.1 -31.09 -37.89 8.02 -7.67 12.4 8.51 9.22 -8.09
374 x 367 -18.62 -49.76 -5.74 -10.94 -41.06 -47.06 -35.25 -60.67 -33.18 -42.25 -37.22 -59.34
374 x 435 -5.84 -40.76 19.78 -4.11 -27.49 -44.49 -45.34 -68.19 27.59 7.25 -23.46 -52.04
374 x 074 -15.78 -21.56 2.93 -14.08 -6.04 -16.74 -32.51 -39.73 10.19 1.34 -24.08 -37.13
367 x 005 -16.67 -45.48 -29.39 -47.49 -19.65 -34.14 -39.96 -65.4 9.47 -8.14 -29.8 -57.82
367 x 374 -13.24 -46.45 -18.5 -22.99 -8.48 -17.8 -47.4 -68.05 -33.18 -42.25 -37.37 -59.44
367 x 435 -2.88 -7.34 -4.18 -26.47 -10.23 -25.19 35.16 19.22 -53.18 -54.71 -37.55 -42.01
367 x 074 -17.93 -47.8 -33.84 -42.03 -25.38 -26.52 -51.21 -71.49 2.49 -4.26 -47.32 -68.56
435 x 005 -29.3 -52.65 -33.73 -58.23 -36.16 -54.48 -64.04 -79.93 12.2 -8.33 -36.62 -62.83
435 x 374 -3.2 -39.1 22.69 -1.79 -27.09 -44.19 -49.03 -70.34 28.88 8.33 -24.32 -52.58
435 x 367 2.88 -1.83 2.3 -21.49 -8.41 -23.67 40.48 23.91 -56.18 -57.61 -35.12 -39.76
435 x 074 2.75 -33.24 -28.45 -49.83 -10.42 -24.41 -47.85 -70.56 -56.4 -60.51 -75.44 -85.68
074 x 005 -11.53 -16.76 2.47 -15.58 -37.97 -49.76 -0.61 -5.45 2.26 -8.93 -2.74 -4.66
074 x 374 -15.27 -21.09 -16.15 -30 5.19 -6.79 -35.96 -42.81 5.83 -2.68 -32.86 -44.4
074 x 367 -17.93 -47.8 -32.48 -40.83 -30.38 -31.44 -48.41 -69.85 2.07 -4.65 -44.59 -66.93
074 x 435 -10.36 -41.76 -3.72 -32.48 2.55 -13.48 -50.47 -72.04 -51.2 -55.8 -75.67 -85.81
FL, fruit length; FD, fruit diameter; FT, fruit thickness; FW, fruit weight; NFP, number of fruits per plant; HMP, Heterotic mid parent (heterotic effect); HHP, Heterotic high parent (heterobeltiosis effect).
Table 12. Heterotic and heterobeltiosis effects for chili biochemical compounds.
Table 12. Heterotic and heterobeltiosis effects for chili biochemical compounds.
Chili Hybrids TPC TFC DPPH FRAP AGI
HMP HHP HMP HHP HMP HHP HMP HHP HMP HHP
.………………………………………… (%) .…………………………………………
005 x 374 -49.24 -51.48 -22.9 -28.41 -2.04 -9.11 -31.27 -38.81 -4.52 -10.41
005 x 367 -42.47 -44.75 -50.94 -60.81 8.38 2.98 -24.68 -27.14 0.71 -9.57
005 x 435 -49.85 -51.7 -31.8 -42.38 3.32 -1.36 -23.39 -28.44 -0.64 -7.29
005 x 074 4.63 -7.49 14.91 12.03 3.1 1.36 -1.92 -5.06 -14.45 -25.27
374 x 005 -48.32 -50.6 -23.68 -29.13 -2.1 -9.17 -33.88 -41.14 -5.24 -11.09
374 x 367 -50.95 -51.19 -60.21 -66.24 10.02 -2.61 -61.68 -64.85 14.69 -2.65
374 x 435 -52.62 -56.31 -42.45 -48.06 -2.25 -5.14 -31.88 -35.33 1.91 -10.35
374 x 074 5.16 -10.58 -20.55 -24.43 4.71 -1.29 -26.33 -36.26 22.68 13.62
367 x 005 -43.96 -46.18 -47.62 -58.16 4.85 -0.37 -27.84 -30.2 -0.64 -10.79
367 x 374 -56.09 -56.31 -60.51 -66.49 8.94 -3.57 -59.53 -62.87 14.24 -3.04
367 x 435 4.65 -3.05 4.73 -2.27 -18.86 -26.21 -3.95 -7.37 3 -1.19
367 x 074 7.96 -7.83 -54.06 -62.58 0.34 -6.18 -55.98 -58.73 3.96 -17.12
435 x 005 -52.3 -54.06 -28.18 -39.32 7.84 2.96 -25.47 -30.38 0.04 -6.65
435 x 374 -51.78 -55.53 -33.41 -39.91 2.11 -0.91 -18.79 -22.89 -1.23 -13.12
435 x 367 2.68 -4.87 5.41 -1.64 -15.93 -23.54 -0.07 -3.62 1.42 -2.7
435 x 074 13.91 4.21 -35.44 -44.28 13.94 10.59 -16.9 -24.69 -0.62 -18.16
074 x 005 0.27 -11.34 12.86 10.03 7.97 6.15 0.54 -2.68 -17.57 -27.99
074 x 374 -1.61 -16.33 -15.03 -19.18 8.99 2.76 -31.3 -40.56 21.92 12.91
074 x 367 11.13 -5.12 -52.66 -61.44 2.34 -4.31 -49.15 -52.33 6.29 -15.26
074 x 435 -1.04 -9.47 -37.46 -46.02 17.73 14.27 -21.47 -28.83 2.34 -15.72
TPC, total phenolic content; TFC, total flavonoid content; DPPH, antioxidant activities DPPH method; FRAP, antioxidant activities FRAP method; AGI, α-glucosidase inhibitor; HMP, Heterotic mid parent (heterotic effect); HHP, Heterotic high parent (heterobeltiosis effect).
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