High-Performance Liquid Chromatography (HPLC): Principles, Applications, Versatality, Efficiency, Innovation and Comparative Analysis in Modern Analytical Chemistry and In Pharmaceutical Sciences

Rashed Ahmed

doi:10.20944/preprints202409.0057.v1

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preprints.org > public health and healthcare > public health and health services > doi: 10.20944/preprints202409.0057.v1 (registering DOI)

Preprint Article Version 1 This version is not peer-reviewed

High-Performance Liquid Chromatography (HPLC): Principles, Applications, Versatality, Efficiency, Innovation and Comparative Analysis in Modern Analytical Chemistry and In Pharmaceutical Sciences

Rashed Ahmed

Version 1 : Received: 1 September 2024 / Approved: 2 September 2024 / Online: 2 September 2024 (08:54:14 CEST)

How to cite: Ahmed, R. High-Performance Liquid Chromatography (HPLC): Principles, Applications, Versatality, Efficiency, Innovation and Comparative Analysis in Modern Analytical Chemistry and In Pharmaceutical Sciences. Preprints 2024, 2024090057. https://doi.org/10.20944/preprints202409.0057.v1 Ahmed, R. High-Performance Liquid Chromatography (HPLC): Principles, Applications, Versatality, Efficiency, Innovation and Comparative Analysis in Modern Analytical Chemistry and In Pharmaceutical Sciences. Preprints 2024, 2024090057. https://doi.org/10.20944/preprints202409.0057.v1

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MDPI and ACS Style

Ahmed, R. High-Performance Liquid Chromatography (HPLC): Principles, Applications, Versatality, Efficiency, Innovation and Comparative Analysis in Modern Analytical Chemistry and In Pharmaceutical Sciences. Preprints 2024, 2024090057. https://doi.org/10.20944/preprints202409.0057.v1

APA Style

Ahmed, R. (2024). High-Performance Liquid Chromatography (HPLC): Principles, Applications, Versatality, Efficiency, Innovation and Comparative Analysis in Modern Analytical Chemistry and In Pharmaceutical Sciences. Preprints. https://doi.org/10.20944/preprints202409.0057.v1

Chicago/Turabian Style

Ahmed, R. 2024 "High-Performance Liquid Chromatography (HPLC): Principles, Applications, Versatality, Efficiency, Innovation and Comparative Analysis in Modern Analytical Chemistry and In Pharmaceutical Sciences" Preprints. https://doi.org/10.20944/preprints202409.0057.v1

Abstract

High-Performance Liquid Chromatography (HPLC) is an advanced analytical technique employed to separate, identify, and quantify components in complex mixtures. The method operates based on the differential affinities of sample molecules for the mobile and stationary phases within a chromatographic column. HPLC is recognized for its high resolution, accuracy, and efficiency, making it indispensable across various fields including pharmaceuticals, biotechnology, environmental analysis, and forensic science. The technique's adaptability to different detectors such as UV-visible, fluorescence, and mass spectrometry enhances its versatility and precision in analyzing a wide array of substances. Despite its advantages, HPLC presents challenges such as high operational costs, the need for specialized expertise, and susceptibility to contamination.Nonetheless, its role in modern analytical chemistry is pivotal, providing crucial insights and supporting developments in diverse scientific and industrial domains. This abstract encapsulates the core aspects of the article, highlighting the significance and applications of HPLC while also acknowledging its limitations.

Keywords

Analytical technique; Chromatography; Liquid chromatography; Separation; Identification; Quantification; Pharmaceuticals; Biotechnology; Environmental analysis; Forensic science; Agrochemicals; Instrumentation; Methodology; Research; Literature review; Case studies; Pharmaceutical Analysis; Pharmaceutical Technology

Subject

Public Health and Healthcare, Public Health and Health Services

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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