DNA Imprinting and Differentially Expressed Genes in Longissimus Thoracis Muscle of Bos indicus Submitted to Early Weaning Management

Gustavo Lucas Bezerra Tinoco; Rogério Abdallah Curi; Marcelo Ricardo Vicari; Gustavo Henrique Russo; Isabella Lissa Iwashita Souza; Juliana Akamine Torrecilhas; Philipe Moriel; Welder Angelo Baldassini; Luis Arthur Loyola Chardulo; Otávio Rodrigues Machado Neto; Guilherme Luis Pereira

doi:10.20944/preprints202409.0207.v1

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preprints.org > biology and life sciences > animal science, veterinary science and zoology > doi: 10.20944/preprints202409.0207.v1

Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

DNA Imprinting and Differentially Expressed Genes in Longissimus Thoracis Muscle of Bos indicus Submitted to Early Weaning Management

Gustavo Lucas Bezerra Tinoco

Rogério Abdallah Curi

Marcelo Ricardo Vicari

Gustavo Henrique Russo

Isabella Lissa Iwashita Souza

Juliana Akamine Torrecilhas

Philipe Moriel

Welder Angelo Baldassini

Luis Arthur Loyola Chardulo

Otávio Rodrigues Machado Neto

Guilherme Luis Pereira

Version 1 : Received: 2 September 2024 / Approved: 3 September 2024 / Online: 4 September 2024 (04:56:42 CEST)

How to cite: Tinoco, G. L. B.; Curi, R. A.; Vicari, M. R.; Russo, G. H.; Souza, I. L. I.; Torrecilhas, J. A.; Moriel, P.; Baldassini, W. A.; Chardulo, L. A. L.; Neto, O. R. M.; Pereira, G. L. DNA Imprinting and Differentially Expressed Genes in Longissimus Thoracis Muscle of Bos indicus Submitted to Early Weaning Management. Preprints 2024, 2024090207. https://doi.org/10.20944/preprints202409.0207.v1 Tinoco, G. L. B.; Curi, R. A.; Vicari, M. R.; Russo, G. H.; Souza, I. L. I.; Torrecilhas, J. A.; Moriel, P.; Baldassini, W. A.; Chardulo, L. A. L.; Neto, O. R. M.; Pereira, G. L. DNA Imprinting and Differentially Expressed Genes in Longissimus Thoracis Muscle of Bos indicus Submitted to Early Weaning Management. Preprints 2024, 2024090207. https://doi.org/10.20944/preprints202409.0207.v1

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MDPI and ACS Style

Tinoco, G. L. B.; Curi, R. A.; Vicari, M. R.; Russo, G. H.; Souza, I. L. I.; Torrecilhas, J. A.; Moriel, P.; Baldassini, W. A.; Chardulo, L. A. L.; Neto, O. R. M.; Pereira, G. L. DNA Imprinting and Differentially Expressed Genes in Longissimus Thoracis Muscle of Bos indicus Submitted to Early Weaning Management. Preprints 2024, 2024090207. https://doi.org/10.20944/preprints202409.0207.v1

APA Style

Tinoco, G. L. B., Curi, R. A., Vicari, M. R., Russo, G. H., Souza, I. L. I., Torrecilhas, J. A., Moriel, P., Baldassini, W. A., Chardulo, L. A. L., Neto, O. R. M., & Pereira, G. L. (2024). DNA Imprinting and Differentially Expressed Genes in Longissimus Thoracis Muscle of<em> Bos indicus</em> Submitted to Early Weaning Management. Preprints. https://doi.org/10.20944/preprints202409.0207.v1

Chicago/Turabian Style

Tinoco, G. L. B., Otávio Rodrigues Machado Neto and Guilherme Luis Pereira. 2024 "DNA Imprinting and Differentially Expressed Genes in Longissimus Thoracis Muscle of<em> Bos indicus</em> Submitted to Early Weaning Management" Preprints. https://doi.org/10.20944/preprints202409.0207.v1

Abstract

Early weaning management followed by energy supplementation can lead to metabolic alterations in the calf that exert long-term effects on the animal’s health and performance. It is believed that the main molecular basis underlying these metabolic adaptations are epigenetic mechanisms that regulate, activate, or silence genes at different stages of development and/or in response to different environmental stimuli. However, little is known about postnatal metabolic programming in Bos indicus. Therefore, this study aimed to compare the DNA methylation profile of Nellore animals submitted to conventional and early weaning and to correlate the findings with genes differentially expressed in Longissimus thoracis skeletal muscle of Bos indicus cattle. A total of 481 differentially methylated regions were identified, with 52% (250) being hypermethylated and 48% (231) hypomethylated. Functional enrichment analysis of 53 differentially methylated and differentially expressed genes was performed. The main enriched terms and pathways were associated with 3’-5’-cyclic adenosine monophosphate (cAMP) signaling which present the upregulated adenylate cyclase 3 (ADCY3) gene and significatively hypomethylated in promoter region. Alterations in cAMP signaling are involved in numerous processes, many of them related to lipid metabolism. The relative differential expression of key genes of this pathway demonstrates the relationship between cAMP signaling and de novo lipogenesis. These findings suggest an important role of postnatal metabolic programming through DNA methylation mechanisms in determining fat deposition in beef.

Keywords

epigenetics; early weaning; adipogenesis; meat

Subject

Biology and Life Sciences, Animal Science, Veterinary Science and Zoology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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