Version 1
: Received: 3 September 2024 / Approved: 3 September 2024 / Online: 3 September 2024 (18:05:27 CEST)
How to cite:
Yoshida, Y.; Aoki, M.; Nagase, K.; Marubashi, K.; Kojima, H.; Itakura, S.; Komatsu, S.; Sugibayashi, K.; Todo, H. Plasmid DNA Delivery into the Skin via Electroporation with a Depot-Type Electrode. Preprints2024, 2024090245. https://doi.org/10.20944/preprints202409.0245.v1
Yoshida, Y.; Aoki, M.; Nagase, K.; Marubashi, K.; Kojima, H.; Itakura, S.; Komatsu, S.; Sugibayashi, K.; Todo, H. Plasmid DNA Delivery into the Skin via Electroporation with a Depot-Type Electrode. Preprints 2024, 2024090245. https://doi.org/10.20944/preprints202409.0245.v1
Yoshida, Y.; Aoki, M.; Nagase, K.; Marubashi, K.; Kojima, H.; Itakura, S.; Komatsu, S.; Sugibayashi, K.; Todo, H. Plasmid DNA Delivery into the Skin via Electroporation with a Depot-Type Electrode. Preprints2024, 2024090245. https://doi.org/10.20944/preprints202409.0245.v1
APA Style
Yoshida, Y., Aoki, M., Nagase, K., Marubashi, K., Kojima, H., Itakura, S., Komatsu, S., Sugibayashi, K., & Todo, H. (2024). Plasmid DNA Delivery into the Skin via Electroporation with a Depot-Type Electrode. Preprints. https://doi.org/10.20944/preprints202409.0245.v1
Chicago/Turabian Style
Yoshida, Y., Kenji Sugibayashi and Hiroaki Todo. 2024 "Plasmid DNA Delivery into the Skin via Electroporation with a Depot-Type Electrode" Preprints. https://doi.org/10.20944/preprints202409.0245.v1
Abstract
Non-viral mediated plasmid DNA transfection by electroporation (EP) is an established method for gene transfection. In this study, the usefulness of direct EP at an intradermal (i.d.) site (DEP) with implanted electrodes to achieve a high protein expression level was investigated. In addition, DEP application with various intervals with a low application voltage was also evaluated to confirm its effect on protein expression. A higher protein expression level was observed after green fluorescent protein (GFP)- and luciferase-encoding DNA were delivered by i.d. injection followed by DEP application. When luciferase expression was observed with an in vivo imaging system, continuous expression was confirmed over 21 days after i.d. injection followed by DEP at 100 V. This approach provided increased gene expression levels compared with conventional EP methods via the stratum corneum layer. In addition, the effect of application voltage on luciferase expression was investigated; two-time applications (repeated DEP) at 20 V with 5 min intervals showed similar luciferase expression level to single DEP application with 100 V. Histological observations showed the skin became thicker after a single DEP at 100 V, whereas no apparent thickness changes was confirmed after repeated DEP at 20 V with 5 min intervals. This study revealed that direct i.d. voltage application achieved high protein expression levels even at low voltages. Skin is a promising administration site for DNA vaccines, so this approach may be effective in DNA vaccine delivery into skin tissue.
Keywords
DNA vaccine; DNA delivery; electroporation; skin; dermal
Subject
Medicine and Pharmacology, Pharmacy
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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